Abstract:
:We used fluorescence in situ hybridization and digital image analysis to localize cosmids along human chromosome 17. Seventy-one cosmids were selected at random from a chromosome 17 library constructed from a partial Sau3AI digest of flow-sorted chromosomes from a mouse-human hybrid cell line. Sixty-three of these (89%) gave a signal only on chromosome 17. The 40 cosmids producing the most distinct hybridization signals in metaphase and interphase cells were precisely mapped using digital image analysis. An additional 20 cosmids, previously mapped by linkage analysis, were also mapped. The order of these probes determined by metaphase mapping was consistent with the order determined by linkage analysis.
journal_name
Genomicsjournal_title
Genomicsauthors
Kallioniemi OP,Kallioniemi A,Mascio L,Sudar D,Pinkel D,Deaven L,Gray Jdoi
10.1006/geno.1994.1138subject
Has Abstractpub_date
1994-03-01 00:00:00pages
125-8issue
1eissn
0888-7543issn
1089-8646pii
S0888-7543(84)71138-4journal_volume
20pub_type
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