Abstract:
:We have characterized 117 cDNAs isolated by direct cDNA selection using pools of human chromosome 12p cosmids. Sequencing revealed that 41 clones did overlap with other cDNAs. Of the remaining 76 cDNA sequences, 11 matched previously identified human chromosome 12p genes and 3 matched previously determined cDNA sequences, including the retinoblastoma binding protein 2 (RBBP2), the cyclin-dependent kinase inhibitor KIP1, and an expressed sequence tag. For each of the 76 cDNAs specific selection by a genomic cosmid clone was confirmed. STSs were developed for all cosmids, among them 3 polymorphic simple sequence repeats associated with, respectively, the TNFR related protein, CD27, and SCNN1. Regional assignment of the STSs by PCR analysis with somatic cell hybrids and fluorescence in situ hybridization showed that the majority of the loci map to chromosome 12p13, similar to the distribution of the known 12p genes. Evidence was found for the duplication on 12p of a region containing a polymorphic simple sequence repeat and sequences of two different cDNAs.
journal_name
Genomicsjournal_title
Genomicsauthors
Baens M,Aerssens J,van Zand K,Van den Berghe H,Marynen Pdoi
10.1006/geno.1995.1213subject
Has Abstractpub_date
1995-09-01 00:00:00pages
44-52issue
1eissn
0888-7543issn
1089-8646pii
S0888-7543(85)71213-Xjournal_volume
29pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Here, we report the complete genomic sequence and the characterization of the 311-kb region of 18q21, a candidate tumor suppressor locus containing a region of homozygous deletion in a lung cancer cell line, Ma29. This region contained two known genes, SMAD4 and ME2 (mitochondrial malate oxydoreductase), and two novel...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6454
更新日期:2001-03-01 00:00:00
abstract::We have analyzed a dense set of single-nucleotide polymorphisms (SNPs) and microsatellites spanning the T-helper cytokine gene cluster (interleukins 3, 4, 5, 9, and 13, interferon regulatory factor-1, colony-stimulating factor-2, and T-cell transcription factor-7) on 5q31 and the gene encoding the interleukin-4 recept...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6613
更新日期:2001-09-01 00:00:00
abstract::Inborn errors of mitochondrial beta-oxidation cause ectopic fat accumulation, particularly in the liver. Fatty liver is associated with insulin resistance and predisposes to hepatic fibrosis. The factors underlying the pathophysiological consequences of hepatic fat accumulation have remained poorly defined. Gene expre...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2007.08.004
更新日期:2007-12-01 00:00:00
abstract::The genes encoding receptors for the chemotactic ligands C5a (C5AR) and FMLP (FPR) were mapped using a panel of somatic cell hybrids to chromosome 19. Because the hybridization pattern on Southern analysis suggested an intron structure or related genes in the case of FPR, genomic clones were characterized. Two structu...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90265-t
更新日期:1992-06-01 00:00:00
abstract::The recently described murine homeobox genes, Six1 and Six2, which are expressed during development in limb tendons, have also been shown to be expressed in skeletal and smooth muscle, respectively. We have cloned and sequenced a human SIX1 cDNA and shown by Northern blotting that it is expressed in adult skeletal mus...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0172
更新日期:1996-04-01 00:00:00
abstract::Most parasitic flatworms go through different life stages with important physiological and morphological changes. In this work, we used a transcriptomic approach to analyze the main life-stages of the model tapeworm Hymenolepis microstoma (eggs, cysticercoids, and adults). Our results showed massive transcriptomic cha...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2021.01.005
更新日期:2021-01-21 00:00:00
abstract::Unbalanced chromosome translocations with breakpoints around 8p12, resulting in loss of distal 8p, are common in carcinomas. We have mapped the 8p12 breakpoints in three breast cancer cell lines, T-47D, MDA-MB-361, and ZR-75-1, using YACs and PACs between D8S540 and D8S255 by fluorescence in situ hybridization. All th...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6178
更新日期:2000-05-15 00:00:00
abstract::Pax-1, a member of a murine multigene family, belongs to the paired box-containing class of developmental control genes first identified in Drosophila. The Pax-1 gene encodes a sequence-specific DNA-binding protein with transcriptional activating properties and has been found to be mutated in the autosomal recessive m...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80177-6
更新日期:1992-11-01 00:00:00
abstract::DNA primase is an essential replication protein that catalyzes the synthesis of oligoribonucleotide primers. DNA primase, consisting of two subunits (p49 and p58), plays a key role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. We mapped the locations of ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1155
更新日期:1995-07-20 00:00:00
abstract::An area of 500 kb at the proximal end of the polycystic kidney disease 1 (PKD1) region has been mapped in detail, with 260 kb cloned in cosmids. The area cloned from normal individuals contains two homologous but divergent regions each of 75 kb, including the previously described marker 26-6. Pulsed-field gel electrop...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1507
更新日期:1994-09-15 00:00:00
abstract::Neogenin was first identified in the chick embryo, and like a number of cell surface proteins of the immunoglobulin (Ig) superfamily, including N-CAM and L1 (generally called cell adhesion molecules or CAMs), it is expressed on growing nerve cells in the developing nervous system of vertebrate embryos. Neogenin is als...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4688
更新日期:1997-05-01 00:00:00
abstract::Development of statistical methods has become very necessary for large-scale correlation analysis in the current "omic" data. We propose ranking analysis of correlation coefficients (RAC) based on transforming correlation matrix into correlation vector and conducting a "locally ranking" strategy that significantly red...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2010.09.002
更新日期:2011-01-01 00:00:00
abstract::The allele-specific polymerase chain reaction (ASPCR) procedure has proven a powerful tool for the detection and analysis of known genetic polymorphisms. Here, we present a novel application of the ASPCR technique to determine the ABO genotypes of individuals without the need of family analysis. The method introduces ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90292-z
更新日期:1992-04-01 00:00:00
abstract::A genetic map of nine loci defined by polymorphic DNA markers was created using a single cross of F344/N and LEW/N rats. The markers contained polymorphic simple sequence repeats identified in five genes, renin (Ren), cardiac troponin T (Tnnt3), synaptotagmin (Syt2), Na+,K(+)-ATPase catalytic subunit (Atp1a2), and the...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1466
更新日期:1993-11-01 00:00:00
abstract::Dynamins, microtubule-binding GTPases, are encoded by at least three genes in mammals. Two distinct gene-specific cDNAs were used to analyze the segregation of dynamin genes Dnm1 and Dnm2 in a mouse interspecies backcross. The nervous system-expressed gene Dnm1 was localized to Chr 2 between the genes for vimentin and...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4634
更新日期:1997-04-15 00:00:00
abstract::The inheritance of obesity has been analyzed in an intercross between the lean 129/Sv mouse strain and the obesity-prone EL/Suz mouse strain. The weights of three major fat pads were determined on 4-month-old mice, and the sum of these weights, divided by body weight, was used as an adiposity index. The strategy of se...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0302
更新日期:1996-06-15 00:00:00
abstract::To refine the linkage map of mouse Chromosome (Chr) 12 and to define better the homology relationships between it and human chrs 2p and 14q, nine new anonymous DNA markers of Chr 12 were identified, and mouse loci homologous to the human D14S17, CHGA, HSPA2, RRM2, TPO, and ZFP50 ("KUP") genes were defined. The inherit...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1234
更新日期:1994-05-01 00:00:00
abstract::The proximal end of mouse chromosome (Chr) 13 contains regions conserved on human chromosomes 1q42-q44, 6p23-p21, and 7p22-p13. This region also contains mutations that may be models for human disease, including beige (human Chediak-Higashi syndrome). An interspecific backcross of SB/Le and Mus spretus mice was used t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.4478
更新日期:1997-01-15 00:00:00
abstract:UNLABELLED:Next-generation sequencing data can be mapped to a reference genome to identify single-nucleotide polymorphisms/variations (SNPs/SNVs; called SNPs hereafter). In theory, SNPs can be compared across several samples and the differences can be used to create phylogenetic trees depicting relatedness among the sa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2014.06.001
更新日期:2014-07-01 00:00:00
abstract::The human chromosomal growth hormone locus contained on cloned DNA and spanning approximately 66,500 bp was sequenced in its entirety to provide a framework for the analysis of its biology and evolution. This locus evolved by a series of duplications and contains in its present form five genes which display a remarkab...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90271-1
更新日期:1989-05-01 00:00:00
abstract::Large deletions in Xq21 often are associated with contiguous gene syndromes consisting of X-linked deafness type 3 (DFN3), mental retardation (MRX), and choroideremia (CHM). The identification of deletions associated with classic CHM or DFN3 facilitated the positional cloning of the underlying genes, REP-1 and POU3F4,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.6004
更新日期:1999-12-15 00:00:00
abstract::alpha-Satellite subfamilies from chromosomes 21 and 13 are almost identical in sequence and cannot be distinguished from each other by hybridization techniques. A general method based on membrane-bound PCR is described here, allowing the discrimination of alpha-satellite DNA sequences from each of these two chromosome...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80256-3
更新日期:1992-10-01 00:00:00
abstract::Deletions giving rise to Duchenne muscular dystrophy (DMD) and the less severe Becker muscular dystrophy (BMD) occur in the same large gene on the short arm of the human X chromosome. We present a molecular mechanism to explain the clinical difference in severity between DMD and BMD patients who bear partial deletions...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(88)90113-9
更新日期:1988-01-01 00:00:00
abstract::Understanding ribosomal protein gene regulation provides a good avenue for understanding gene regulatory networks. Even after 5 decades of research on ribosomal protein gene regulation, little is known about how higher eukaryotic ribosomal protein genes are coordinately regulated at the transcriptional level. However,...
journal_title:Genomics
pub_type: 杂志文章,评审
doi:10.1016/j.ygeno.2007.07.003
更新日期:2007-10-01 00:00:00
abstract::Runs of homozygosity are common in European populations and are indicative of consanguinity, restricted population size and recessively inherited traits. Here, we map runs of homozygosity (ROHs) in an Irish case-control cohort for amyotrophic lateral sclerosis (ALS), a devastating neurological condition with high heri...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2015.01.002
更新日期:2015-04-01 00:00:00
abstract::We completed a systematic survey of the human genome, conducted at an average resolution of 10 cM, for the identification of simple sequence tandem repeat polymorphisms (SSTRPs) that target new risk genes for Alzheimer disease (AD) by virtue of linkage disequilibrium. The efficiency of our association study was enhanc...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5306
更新日期:1998-06-01 00:00:00
abstract::We report the results of a chemogenomic profiling aimed to explore the mode of action of a quinolic analogue of the p300 histone acetyltransferase (HAT) inhibitor anacardic acid, named MC1626. This compound reduced histone H3 acetylation in a dose-dependent manner and the HATs Gcn5 and Rtt109, which specifically targe...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2010.08.005
更新日期:2010-11-01 00:00:00
abstract::The canine species, including wolf and jackal, have four digits on the hind limb. It was thought that an extra first digit on the hind limb, named dewclaw, was a hereditary defect. For genetically related canine pedigrees with 73 members with dewclaws, we carried out a genome-wide scan for linkage with microsatellites...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00234-9
更新日期:2004-02-01 00:00:00
abstract::Calcyclin is a member of the S100 family of proteins, many of which are encoded by genes that have been localized to the proximal long arm of human chromosome 1 (bands q21-q22). A 450-kb yeast artificial chromosome clone containing the human calcyclin gene was identified by PCR screening and used as a probe for fluore...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1277
更新日期:1994-05-15 00:00:00
abstract::Using antibodies directed against the amino-terminus of dystrophin, we identified a truncated protein in a Duchenne muscular dystrophy patient. Antibodies directed against the carboxy-terminus failed to identify any cross-reactive material, a result consistent with premature termination of dystrophin translation. The ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90332-9
更新日期:1991-06-01 00:00:00