Abstract:
:It is hypothesized that autosomal retroposons compensate for the loss of their inactivated essential X-chromosome progenitors during spermatogenesis. Here we test this Retroposon Compensatory Mechanism (RCM) hypothesis using the Zfy gene family. The mouse autosomal retroposon Zfa is expressed in testes at the same developmental time points at which Zfx levels decline, which correspond to the time of male sex chromosome inactivation, suggesting that Zfa may compensate for the loss of Zfx during spermatogenesis. We examined the effect of Zfa-targeted mutagenesis on spermatogenesis in three genetically distinct mouse strains. Surprisingly, Zfa knockout mice showed no detectable fertility, sperm count, or testes morphology defects. We therefore conclude that Zfa is not an essential gene for spermatogenesis and fertility. This surprising finding now challenges the RCM hypothesis at least for the Zfy gene family. It also forces us to reevaluate the original data underpinning the RCM hypothesis for this family and to propose alternative hypotheses.
journal_name
Genomicsjournal_title
Genomicsauthors
Banks KG,Johnson KA,Lerner CP,Mahaffey CL,Bronson RT,Simpson EMdoi
10.1016/s0888-7543(03)00155-1subject
Has Abstractpub_date
2003-09-01 00:00:00pages
254-60issue
3eissn
0888-7543issn
1089-8646pii
S0888754303001551journal_volume
82pub_type
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