Abstract:
:We present a rapid and efficient method for the isolation of minisatellite loci from human DNA. The method combines cloning a size-selected fraction of human MboI DNA fragments in a charomid vector with hybridization screening of the library in ordered array. Size-selection of large MboI fragments enriches for the longer, more variable minisatellites and reduces the size of the library required. The library was screened with a series of multi-locus probes known to detect a large number of hypervariable loci in human DNA. The gridded library allowed both the rapid processing of positive clones and the comparative evaluation of the different multi-locus probes used, in terms of both the relative success in detecting hypervariable loci and the degree of overlap between the sets of loci detected. We report 23 new human minisatellite loci isolated by this method, which map to 14 autosomes and the sex chromosomes.
journal_name
Genomicsjournal_title
Genomicsauthors
Armour JA,Povey S,Jeremiah S,Jeffreys AJdoi
10.1016/0888-7543(90)90037-usubject
Has Abstractpub_date
1990-11-01 00:00:00pages
501-12issue
3eissn
0888-7543issn
1089-8646pii
0888-7543(90)90037-Ujournal_volume
8pub_type
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