Abstract:
:Methyl-CpG binding domain proteins (MBD) can specifically bind to methylated CpG sites and play important roles in epigenetic gene regulation. Here, we identified and functionally characterized the MBD protein in Tribolium castaneum. T. castaneum genome encodes only one MBD protein: TcMBD2/3. RNA interference targeting this gene at different developmental stages caused lethal phenotypes including metamorphosis deficiency in larvae and pupae, gastrointestinal system problems and fecundity deficiency in adult. Moreover, Tcmbd2/3 knockdown adult showed progressive reduced locomoter activity, a typical neurodegeneration phenotype. This is a common feature of DNA methylation in mammals and has not been found in other insects. However, band shift assays demonstrated that TcMBD2/3 could not bind to methylated DNA, indicating the essential roles of TcMBD2/3 is independent of DNA methylation. Our study provides Tcmbd2/3 plays important roles in T. castaneum and gives new insights into the potential mechanism of action of MBD proteins in insect.
journal_name
Genomicsjournal_title
Genomicsauthors
Song X,Zhang Y,Zhong Q,Zhan K,Bi J,Tang J,Xie J,Li Bdoi
10.1016/j.ygeno.2019.12.018subject
Has Abstractpub_date
2020-05-01 00:00:00pages
2223-2232issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(19)30353-2journal_volume
112pub_type
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