abstract：:COVID-19 pandemic, caused by SARS-CoV-2, has drastically affected human health all over the world. After the emergence of the pandemic the major focus of efforts to attenuate the infection has been on repurposing the already approved drugs to treat COVID-19 adopting a fast-track strategy. However, to date a specific r...

journal_title：Journal of structural biology

authors： Shahid M,Shahzad-Ul-Hussan S

更新日期：2020-12-29 00:00:00

abstract：:Understanding skeletal aging and predicting fracture risk is increasingly important with a growing elderly population. We hypothesized that when categorized by external bone size, the male femoral diaphysis would show different strength-age trajectories which can be explained by changes in morphology, composition and ...

journal_title：Journal of structural biology

authors： Bolger MW,Romanowicz GE,Bigelow EMR,Ward FS,Ciarelli A,Jepsen KJ,Kohn DH

更新日期：2020-12-01 00:00:00

abstract：:The bacterial injectisome and flagella both rely on type III secretion systems for their assembly. The syringe-like injectisome creates a continuous channel between the bacterium and the host cell, through which signal-modulating effector proteins are secreted. The inner membrane pore protein SctV controls the hierarc...

journal_title：Journal of structural biology

authors： Majewski DD,Lyons BJE,Atkinson CE,Strynadka NCJ

更新日期：2020-12-01 00:00:00

abstract：:Studying nanostructured hierarchical materials such as the biomineralized bone is challenging due to their complex 3D structures that call for high spatial resolution. One route to study such materials is X-ray powder diffraction computed tomography (XRD-CT) that reveals the 3D distribution of crystalline phases and X...

journal_title：Journal of structural biology

authors： Palle J,Wittig NK,Kubec A,Niese S,Rosenthal M,Burghammer M,Grünewald TA,Birkedal H

更新日期：2020-12-01 00:00:00

abstract：:Pineal gland (PG) is a part of the human brain epithalamus that plays an important role in sleep, circadian rhythm, immunity, and reproduction. The calcium deposits and lesions in PG interfere with normal function of the organ and can be associated with different health disorders including serious neurological disease...

journal_title：Journal of structural biology

authors： Bukreeva I,Junemann O,Cedola A,Palermo F,Maugeri L,Begani Provinciali G,Pieroni N,Sanna A,Otlyga DA,Buzmakov A,Krivonosov Y,Zolotov D,Chukalina M,Ivanova A,Saveliev S,Asadchikov V,Fratini M

更新日期：2020-12-01 00:00:00

abstract：:Enameloid, the hyper-mineralized tissue covering shark teeth is a complex structure resulting from both ameloblast and odontoblast activity. The way these two types of cells interact to set up this tissue is not fully understood and results in the formation of subunits in the enameloid: the Single Crystallite Enameloi...

journal_title：Journal of structural biology

authors： Fellah C,Douillard T,Maire E,Meille S,Reynard B,Cuny G

更新日期：2020-11-19 00:00:00

abstract：:Sialic acid residues found as terminal monosaccharides in various types of glycan chains in cell surface glycoproteins and glycolipids have been identified as important contributors of cell-cell interactions in normal vs. abnormal cellular behavior and are pivotal in diseases such as cancers. In vertebrates, sialic ac...

journal_title：Journal of structural biology

authors： Harrus D,Harduin-Lepers A,Glumoff T

更新日期：2020-11-01 00:00:00

abstract：:Bacterial two-component regulatory systems (TCS) play important roles in sensing environmental stimuli and responding to them by regulating gene expression. VbrK/VbrR, a TCS in Vibrio parahaemolyticus, confers resistance to β-lactam antibiotics through activating a β-lactamase gene. Its periplasmic sensor domain was p...

journal_title：Journal of structural biology

authors： Goh BC,Chua YK,Qian X,Lin J,Savko M,Dedon PC,Lescar J

更新日期：2020-11-01 00:00:00

abstract：:Like in most Crustacea, the cuticle of terrestrial isopods is hardened by a calcareous mineral phase. This rigid cuticle is frequently shed during a process called moulting. To reduce calcium loss, Porcellio scaber eats the shed cuticle, the exuviae, and absorb the calcium from it through large tubular diverticula of ...

journal_title：Journal of structural biology

authors： Rupp U,Walther P,Ziegler A

更新日期：2020-11-01 00:00:00

abstract：:Hedgehog signaling plays a crucial role in embryogenesis and adult tissue homeostasis, and mutations of its key components such as Suppressor of fused (Sufu) are closely associated with human diseases. The Ser/Thr kinase Fused (Fu) promotes Hedgehog signaling by phosphorylating the Cubitus interruptus (Ci)/Glioma-asso...

journal_title：Journal of structural biology

authors： Li H,Wang W,Zhang W,Wu G

更新日期：2020-11-01 00:00:00

abstract：:DciA is a newly discovered bacterial protein involved in loading the replicative helicase DnaB onto DNA at the initiation step of chromosome replication. Its three-dimensional structure is composed of a folded N-terminal domain (residues 1-111) resembling K Homology domains and a long disordered C-terminal tail (resid...

journal_title：Journal of structural biology

authors： Chan-Yao-Chong M,Marsin S,Quevillon-Cheruel S,Durand D,Ha-Duong T

更新日期：2020-10-01 00:00:00

abstract：:BCP1 is a protein enriched in the nucleus that is required for Mss4 nuclear export and identified as the chaperone of ribosomal protein Rpl23 in Saccharomyces cerevisiae. According to sequence homology, BCP1 is related to the mammalian BRCA2-interacting protein BCCIP and belongs to the BCIP protein family (PF13862) in...

journal_title：Journal of structural biology

authors： Lin MH,Kuo PC,Chiu YC,Chang YY,Chen SC,Hsu CH

更新日期：2020-10-01 00:00:00

abstract：:We examine the structure of the bone of the pharyngeal jaws of a large fish, the black drum (Pogonias cromis), that uses its tooth-jaw complex to crush hard-shelled bivalve mollusks. During mastication huge compressive forces are concentrated in a tiny zone at the tooth-bone interface. We report on the structure of th...

journal_title：Journal of structural biology

authors： Raguin E,Rechav K,Brumfeld V,Shahar R,Weiner S

更新日期：2020-08-01 00:00:00

abstract：:The Membrane Attack Complex-Perforin (MACPF) family is ubiquitously found in all kingdoms. They have diverse cellular roles, however MACPFs with pore-forming toxic function in venoms and poisons are very rare in animals. Here we present the structure of PmPV2, a MACPF toxin from the poisonous apple snail eggs, that ca...

journal_title：Journal of structural biology

authors： Giglio ML,Ituarte S,Milesi V,Dreon MS,Brola TR,Caramelo J,Ip JCH,Maté S,Qiu JW,Otero LH,Heras H

更新日期：2020-08-01 00:00:00

abstract：:In many organisms, the ubiquitous second messenger cAMP is formed by at least one member of the adenylyl cyclase (AC) Class III. These ACs feature a conserved dimeric catalytic core architecture, either through homodimerization or through pseudo-heterodimerization of a tandem of two homologous catalytic domains, C1 an...

journal_title：Journal of structural biology

authors： Linder J,Hupfeld E,Weyand M,Steegborn C,Moniot S

更新日期：2020-08-01 00:00:00

abstract：:Dipeptidase 3 (DPEP3) is one of three glycosylphosphatidylinositol-anchored metallopeptidases potentially involved in the hydrolytic metabolism of dipeptides. While its exact biological function is not clear, DPEP3 expression is normally limited to testis, but can be elevated in ovarian cancer. Antibody drug conjugate...

journal_title：Journal of structural biology

authors： Hayashi K,Longenecker KL,Koenig P,Prashar A,Hampl J,Stoll V,Vivona S

更新日期：2020-07-01 00:00:00

abstract：:The central shaft of a bird's flight feather bears most of the aerodynamic load during flight and exhibits some remarkable mechanical properties. The shaft comprises two parts, the calamus and the rachis. The calamus is at the base of the shaft, while the rachis is the longer upper part which supports the vanes. The s...

journal_title：Journal of structural biology

authors： Laurent CM,Dyke JM,Cook RB,Dyke G,de Kat R

更新日期：2020-07-01 00:00:00

abstract：:The analysis of structure factors in 3D cryo-EM Coulomb potential maps and their "enhancement" at the end of the reconstruction process is a well-established practice, normally referred to as sharpening. The aim is to increase contrast and, in this way, to help tracing the atomic model. The most common way to accompli...

journal_title：Journal of structural biology

authors： Vilas JL,Vargas J,Martinez M,Ramirez-Aportela E,Melero R,Jimenez-Moreno A,Garduño E,Conesa P,Marabini R,Maluenda D,Carazo JM,Sorzano COS

更新日期：2020-03-01 00:00:00

abstract：:DivIVA or Wag31, which is an essential pole organizing protein in mycobacteria, can self-assemble at the negatively curved side of the membrane at the growing pole to form a higher order structural scaffold for maintaining cellular morphology and localizing various target proteins for cell-wall biogenesis. The structu...

journal_title：Journal of structural biology

authors： Choukate K,Gupta A,Basu B,Virk K,Ganguli M,Chaudhuri B

更新日期：2020-02-01 00:00:00

abstract：:This study demonstrates the effects of progesterone on eggshell quality and ultrastructure by injecting progesterone into laying hens 2 and 5 h post-oviposition, respectively. Progesterone injected 2 h post-oviposition (P4-2 h) improved eggshell quality with a significant decrease (P < 0.01) in the thickness of the ma...

journal_title：Journal of structural biology

authors： Zhang J,Wang Z,Wang X,Sun L,Rajput SA,Qi D

更新日期：2020-02-01 00:00:00

abstract：:Annexins are soluble cytosolic proteins that bind to cell membranes. Annexin A5 self-assembles into a two-dimensional (2D) array and prevents cell rupture by attaching to damaged membranes. However, this process is not fully understood at the molecular level. In this study, we determined the crystal structures of anne...

journal_title：Journal of structural biology

authors： Hong S,Na S,Kim OH,Jeong S,Oh BC,Ha NC

更新日期：2020-01-01 00:00:00

abstract：:Computational modeling of membrane proteins is critical to understand biochemical systems and to support chemical biology. In this work, we use a dataset of 448 non-redundant membrane protein chains to expose a "rule" that governs membrane protein structure: free cysteine thiols are not found accessible to oxidative c...

journal_title：Journal of structural biology

authors： Grazhdankin E,Stepniewski M,Xhaard H

更新日期：2020-01-01 00:00:00

abstract：:Staphylococcus aureus hibernation promoting factor (SaHPF) is responsible for the formation of 100S ribosome dimers, which in turn help this pathogen to reduce energy spent under unfavorable conditions. Ribosome dimer formation strongly depends on the dimerization of the C-terminal domain of SaHPF (CTDSaHPF). In this ...

journal_title：Journal of structural biology

authors： Usachev KS,Fatkhullin BF,Klochkova EA,Miftakhov AK,Golubev AA,Bikmullin AG,Nurullina LI,Garaeva NS,Islamov DR,Gabdulkhakov AG,Lekontseva NV,Tishchenko SV,Balobanov VA,Khusainov IS,Yusupov MM,Validov SZ

更新日期：2020-01-01 00:00:00

abstract：:The formation of amyloid is considered an intrinsic ability of most polypeptides. It is a structure adopted by many neuropeptides and neurohormones during the formation of dense core vesicles in secretory cells, yet the mechanisms mediating assembly and disassembly of these amyloids remain unclear. Neurokinin B is a n...

journal_title：Journal of structural biology

authors： Jayawardena BM,Jones MR,Hong Y,Jones CE

更新日期：2019-12-01 00:00:00

abstract：:The power of cryo-electron tomography (cryoET) lies in its capability to characterize macromolecules in their cellular context. Structure determination by cryoET, however, is time-consuming compared to single particle approaches. A recent study reported significant acceleration of data acquisition by a fast-incrementa...

journal_title：Journal of structural biology

authors： Eisenstein F,Danev R,Pilhofer M

更新日期：2019-11-01 00:00:00

abstract：:Low complexity regions (LCRs) in protein sequences have special properties that are very different from those of globular proteins. The rules that define secondary structure elements do not apply when the distribution of amino acids becomes biased. While there is a tendency towards structural disorder in LCRs, various...

journal_title：Journal of structural biology

authors： Kamel M,Mier P,Tari A,Andrade-Navarro MA

更新日期：2019-11-01 00:00:00

abstract：:The β-carbonic anhydrases (β-CAs) are widely distributed zinc-metalloenzymes that play essential roles in growth, survival, development and virulence in fungi. The majority of filamentous ascomycetes possess multiple β-CA isoforms among which major and minor forms have been characterized. We examined the catalytic beh...

journal_title：Journal of structural biology

authors： Kim S,Kim NJ,Hong S,Kim S,Sung J,Jin MS

更新日期：2019-10-01 00:00:00

abstract：:Yersinia enterocolitica is a pectinolytic zoonotic foodborne pathogen, the genome of which contains pectin-binding proteins and several different classes of pectinases, including polysaccharide lyases (PLs) and an exopolygalacturonase. These proteins operate within a coordinated pathway to completely saccharify homoga...

journal_title：Journal of structural biology

authors： Jones DR,McLean R,Hobbs JK,Abbott DW

更新日期：2019-09-01 00:00:00

abstract：:Non-collagenous proteins such as osteocalcin function as regulators of the mineralization process in bone. Osteocalcin undergoes post-translational modification adding an extra carboxylate group on three of its glutamate residues to enhance interaction with bone mineral. In this work, we examine regulation of biomimet...

journal_title：Journal of structural biology

authors： Iline-Vul T,Kulpanovich A,Nadav-Tsubery M,Semionov A,Keinan-Adamsky K,Goobes G

更新日期：2019-08-01 00:00:00

abstract：:Mononuclear macrophages are important immune cells in the organisms. The complicated membrane structure underlying the diverse functions of mononuclear-macrophage has been largely unresolved. As a representative of monocyte-derived macrophages, the membrane structure of PMA differentiated THP-1 cells was comprehensive...

journal_title：Journal of structural biology

authors： Tian Y,Wu Y,Liu L,He L,Gao J,Zhou L,Yu F,Yu S,Wang H

更新日期：2019-06-01 00:00:00

abstract：:REMORINs are nanodomain-organized proteins located in the plasma membrane and involved in cellular responses in plants. The dynamic assembly of the membrane nanodomains represents an essential tool of the versatile membrane barriers to control and modulate cellular functions. Nevertheless, the assembly mechanisms and ...

journal_title：Journal of structural biology

authors： Martinez D,Legrand A,Gronnier J,Decossas M,Gouguet P,Lambert O,Berbon M,Verron L,Grélard A,Germain V,Loquet A,Mongrand S,Habenstein B

更新日期：2019-04-01 00:00:00

abstract：:Human scalp hairs are often examined microscopically to study the variation and diversity among a range of visible morphological traits. In this study, we focused on the ultrastructure of human scalp hair within its keratinized matrix, emphasizing, the density and distribution of melanosomes, variation in cuticle thic...

journal_title：Journal of structural biology

authors： Koch SL,Shriver MD,Jablonski NG

更新日期：2019-01-01 00:00:00

abstract：:Cryogenic electron microscopy (cryo-EM) and single-particle analysis enables determination of near-atomic resolution structures of biological molecules. However, large computational requirements limit throughput and rapid testing of new image processing tools. We developed PRIME, an algorithm part of the SIMPLE softwa...

journal_title：Journal of structural biology

authors： Reboul CF,Kiesewetter S,Eager M,Belousoff M,Cui T,De Sterck H,Elmlund D,Elmlund H

更新日期：2018-11-01 00:00:00

abstract：:The transcriptional regulator CpxR mediates an adaptive response to envelope stress, tightly linked to virulence and antibiotics resistance in several Gammaproteobacteria pathogens. In this work, we integrated crystallographic and small-angle X-ray scattering data to gain insights into the structure and conformational...

journal_title：Journal of structural biology

authors： Mechaly AE,Haouz A,Sassoon N,Buschiazzo A,Betton JM,Alzari PM

更新日期：2018-11-01 00:00:00

abstract：:In COPII mediated vesicle formation, Sec13/Sec31 heterotetramers play a role in organizing the membranes into a spherical vesicle. There they oligomerize into a cage that interacts with the other COPII proteins to direct vesicle formation and concentrate cargo into a bud. In this role they must be flexible to accommod...

journal_title：Journal of structural biology

authors： Paraan M,Bhattacharya N,Uversky VN,Stagg SM

更新日期：2018-11-01 00:00:00

abstract：:Protein tertiary structure modeling is a critical step for the interpretation of three dimensional (3D) election microscopy density. Our group participated the 2015/2016 EM Model Challenge using the MAINMAST software for a de novo main chain modeling. The software generates local dense points using the mean shifting a...

journal_title：Journal of structural biology

authors： Terashi G,Kihara D

更新日期：2018-11-01 00:00:00

abstract：:The Bsoft package is aimed at processing electron micrographs for the determination of the three-dimensional structures of biological specimens. Recent advances in hardware allow us to solve structures to near atomic resolution using single particle analysis (SPA). The Map Challenge offered me an opportunity to test t...

journal_title：Journal of structural biology

authors： Heymann JB

更新日期：2018-10-01 00:00:00

abstract：:An evaluation system and a web infrastructure were developed for the second cryo-EM model challenge. The evaluation system includes tools to validate stereo-chemical plausibility of submitted models, check their fit to the corresponding density maps, estimate their overall and per-residue accuracy, and assess their si...

journal_title：Journal of structural biology

authors： Kryshtafovych A,Adams PD,Lawson CL,Chiu W

更新日期：2018-10-01 00:00:00

abstract：:To construct calcium carbonate skeletons of sophisticated architecture, scleractinian corals secrete an extracellular skeletal organic matrix (SOM) from aboral ectodermal cells. The SOM, which is composed of proteins, saccharides, and lipids, performs functions critical for skeleton formation. Even though polysacchari...

journal_title：Journal of structural biology

authors： Takeuchi T,Plasseraud L,Ziegler-Devin I,Brosse N,Shinzato C,Satoh N,Marin F

更新日期：2018-09-01 00:00:00

abstract：:Gly missense mutations in type I collagen, which replace a conserved Gly in the repeating (Gly-Xaa-Yaa)n sequence with a larger residue, are known to cause Osteogenesis Imperfecta (OI). The clinical consequences of such mutations range from mild to lethal, with more serious clinical severity associated with larger Gly...

journal_title：Journal of structural biology

authors： Qiu Y,Mekkat A,Yu H,Yigit S,Hamaia S,Farndale RW,Kaplan DL,Lin YS,Brodsky B

更新日期：2018-09-01 00:00:00