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The structural characteristics of mononuclear-macrophage membrane observed by atomic force microscopy.

Abstract：

:Mononuclear macrophages are important immune cells in the organisms. The complicated membrane structure underlying the diverse functions of mononuclear-macrophage has been largely unresolved. As a representative of monocyte-derived macrophages, the membrane structure of PMA differentiated THP-1 cells was comprehensively investigated by AFM-based single molecule approaches. The rugged ectoplasmic side of mononuclear-macrophage membrane are significantly different from erythrocytes and mammalian somatic cell membranes. But the smooth lipid bilayer and the branched lipid raft domains obtained by proteinase K and MβCD treatment of the protein-covered cytoplasmic side, are common characteristics among all the studied cell membranes. This discovery of distinct organization of membrane proteins on both sides of mononuclear-macrophage membranes provides additional evidence for the asymmetry of membrane structure. The podosome-associated structures of mononuclear-macrophage were directly examined, and the independent localization of podosome domains and the lipid rafts was verified by in situ AFM, giving new insight into this multifunctional organelle.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Tian Y,Wu Y,Liu L,He L,Gao J,Zhou L,Yu F,Yu S,Wang H

doi

10.1016/j.jsb.2019.04.002

subject

Has Abstract

pub_date

2019-06-01 00:00:00

pages

314-321

issue

3

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(19)30068-1

journal_volume

206

pub_type

杂志文章

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