当前位置： SCI文献检索 > JOURNAL OF STRUCTURAL BIOLOGY期刊下所有文献 > Cryo-electron tomography of nanoparticle transmigration into liposome.

Cryo-electron tomography of nanoparticle transmigration into liposome.

Abstract：

:Nanoparticle transport across cell membrane plays a crucial role in the development of drug delivery systems as well as in the toxicity response induced by nanoparticles. As hydrophilic nanoparticles interact with lipid membranes and are able to induce membrane perturbations, hypothetic mechanisms based on membrane curvature or hole formation have been proposed for activating their transmigration. We report on the transport of hydrophilic silica nanoparticles into large unilamellar neutral DOPC liposomes via an internalization process. The strong adhesive interactions of lipid membrane onto the silica nanoparticle triggered liposome deformation until the formation of a curved neck. Then the rupture of this membrane neck led to the complete engulfment of the nanoparticle. Using cryo-electron tomography we determined 3D architectures of intermediate steps of this process unveiling internalized silica nanoparticles surrounded by a supported lipid bilayer. This engulfing process was achieved for a large range of particle size (from 30 to 200 nm in diameter). These original data provide interesting highlights for nanoparticle transmigration and could be applied to biotechnology development.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Le Bihan O,Bonnafous P,Marak L,Bickel T,Trépout S,Mornet S,De Haas F,Talbot H,Taveau JC,Lambert O

doi

10.1016/j.jsb.2009.07.006

subject

Has Abstract

pub_date

2009-12-01 00:00:00

pages

419-25

issue

3

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(09)00176-2

journal_volume

168

pub_type

杂志文章

相关文献

JOURNAL OF STRUCTURAL BIOLOGY文献大全
  • Functionalization of biomineral reinforcement in crustacean cuticle: Calcite orientation in the partes incisivae of the mandibles of Porcellio scaber and the supralittoral species Tylos europaeus (Oniscidea, Isopoda).

    abstract：:In arthropods the cuticle forms an exoskeleton with its physical and chemical properties adapted to functions of distinct skeletal elements. The cuticle of the partes incisivae (PI) in mandibles of terrestrial isopods is a composite of chitin-protein fibrils/fibres and minerals. It consists of an unmineralized tip, a ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2015.03.007

    authors： Huber J,Griesshaber E,Nindiyasari F,Schmahl WW,Ziegler A

    更新日期：2015-05-01 00:00:00

  • Structural and functional characterization of two unusual endonuclease III enzymes from Deinococcus radiodurans.

    abstract：:While most bacteria possess a single gene encoding the bifunctional DNA glycosylase Endonuclease III (EndoIII) in their genomes, Deinococcus radiodurans possesses three: DR2438 (DrEndoIII1), DR0289 (DrEndoIII2) and DR0982 (DrEndoIII3). Here we have determined the crystal structures of DrEndoIII1 and an N-terminally tr...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2015.05.009

    authors： Sarre A,Ökvist M,Klar T,Hall DR,Smalås AO,McSweeney S,Timmins J,Moe E

    更新日期：2015-08-01 00:00:00

  • Crystal structure of the periplasmic sensor domain of histidine kinase VbrK suggests indirect sensing of β-lactam antibiotics.

    abstract：:Bacterial two-component regulatory systems (TCS) play important roles in sensing environmental stimuli and responding to them by regulating gene expression. VbrK/VbrR, a TCS in Vibrio parahaemolyticus, confers resistance to β-lactam antibiotics through activating a β-lactamase gene. Its periplasmic sensor domain was p...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2020.107610

    authors： Goh BC,Chua YK,Qian X,Lin J,Savko M,Dedon PC,Lescar J

    更新日期：2020-11-01 00:00:00

  • The micrograph data processing program.

    abstract：:MDPP is a fully featured general-purpose image processing package primarily written to support research in structural biology using data gathered by electron microscopy. It has focused on the analysis of images using Fourier techniques, particularly periodic plane-layer and helical structures, but has extensive tools ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章,评审

    doi：10.1006/jsbi.1996.0007

    authors： Smith PR,Gottesman SM

    更新日期：1996-01-01 00:00:00

  • Architecture of native human alpha 2-macroglobulin studied by cryoelectron microscopy and three-dimensional reconstruction.

    abstract：:The architecture of the native human alpha 2-macroglobulin was studied by cryoelectron microscopy and image processing techniques. The lip, padlock, doughnut, and four-petaled flower views of this homotetrameric proteinase inhibitor were observed in the frozen-hydrated specimen, and a new view, termed eye view, was al...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1994.1034

    authors： Larquet E,Boisset N,Pochon F,Lamy J

    更新日期：1994-07-01 00:00:00

  • Biochemical characterization of the skeletal matrix of the massive coral, Porites australiensis - The saccharide moieties and their localization.

    abstract：:To construct calcium carbonate skeletons of sophisticated architecture, scleractinian corals secrete an extracellular skeletal organic matrix (SOM) from aboral ectodermal cells. The SOM, which is composed of proteins, saccharides, and lipids, performs functions critical for skeleton formation. Even though polysacchari...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.05.011

    authors： Takeuchi T,Plasseraud L,Ziegler-Devin I,Brosse N,Shinzato C,Satoh N,Marin F

    更新日期：2018-09-01 00:00:00

  • The 17A structure of the 420 kDa lobster clottable protein by single particle reconstruction from cryoelectron micrographs.

    abstract：:Crustaceans form clots by the rapid crosslinking of a hemolymph clottable protein (CP) to form long, branched polymers. Clotting limits hemolymph loss from wounds as well as playing a part in the innate immune response. CP is a 420 kDa homodimer with a large quantity of associated lipid, primarily the carotenoid pigme...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2005.06.008

    authors： Kollman JM,Quispe J

    更新日期：2005-09-01 00:00:00

  • Application of isotope exchange based mass spectrometry to understand the mechanism of inhibition of sickle hemoglobin polymerization upon oxygenation.

    abstract：:Sickle hemoglobin (HbS) polymerization initiates in the deoxy state with the binding of hydrophobic patch formed by the isopropyl group of βVal6 residue of a hemoglobin tetramer with the hydrophobic pocket of another tetramer, whose hydrophobic patch binds to the hydrophobic groove of a third molecule. Subsequent elon...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2017.04.009

    authors： Das R,Mitra A,Bhat V,Mandal AK

    更新日期：2017-07-01 00:00:00

  • Crystal structure of pentapeptide-independent chemotaxis receptor methyltransferase (CheR) reveals idiosyncratic structural determinants for receptor recognition.

    abstract：:Chemotactic methyltransferase, CheR catalyse methylation of specific glutamate residues in the cytoplasmic domain of methyl-accepting chemotactic protein receptors (MCPRs). The methylation of MCPRs is essential for the chemical sensing and chemotactic bacterial mobility towards favorable chemicals or away from unfavor...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2016.08.005

    authors： Batra M,Sharma R,Malik A,Dhindwal S,Kumar P,Tomar S

    更新日期：2016-12-01 00:00:00

  • In situ detection of specific gene expression during and immediately after transcription at electron microscopic level.

    abstract：:In situ hybridization (ISH) is a widely applied technique used for visualizing specific nucleic acid sequences at chromosomal, cytologic, and histologic levels. It sometimes fails, however, to demonstrate precise cell identity, early stages of gene expression and variants of alternative splicing because of its limited...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2005.09.010

    authors： Kitazawa S,Kitazawa R

    更新日期：2006-01-01 00:00:00

  • Heterogeneous nanostructural and nanoelastic properties of pericellular and interterritorial matrices of chondrocytes by atomic force microscopy.

    abstract：:Hyaline cartilage consists of sparse chondrocytes and abundant extracellular matrix. There is a paucity of experimental data in support of the notion of conceivable regional differences in the mechanical properties of chondral matrices. Upon visual differentiation of the pericellular and interterritorial matrices in e...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2003.10.003

    authors： Allen DM,Mao JJ

    更新日期：2004-03-01 00:00:00

  • Single particle reconstruction and validation using Bsoft for the map challenge.

    abstract：:The Bsoft package is aimed at processing electron micrographs for the determination of the three-dimensional structures of biological specimens. Recent advances in hardware allow us to solve structures to near atomic resolution using single particle analysis (SPA). The Map Challenge offered me an opportunity to test t...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.07.003

    authors： Heymann JB

    更新日期：2018-10-01 00:00:00

  • The combination of chemical fixation procedures with high pressure freezing and freeze substitution preserves highly labile tissue ultrastructure for electron tomography applications.

    abstract：:The emergence of electron tomography as a tool for three dimensional structure determination of cells and tissues has brought its own challenges for the preparation of thick sections. High pressure freezing in combination with freeze substitution provides the best method for obtaining the largest volume of well-preser...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2007.09.002

    authors： Sosinsky GE,Crum J,Jones YZ,Lanman J,Smarr B,Terada M,Martone ME,Deerinck TJ,Johnson JE,Ellisman MH

    更新日期：2008-03-01 00:00:00

  • Discontinuous membrane helices in transport proteins and their correlation with function.

    abstract：:Alpha-helical bundles and beta-barrel proteins represent the two basic types of architecture known for integral membrane proteins. Irregular structural motifs have been revealed with the growing number of structures determined. "Discontinuous" helices are present in membrane proteins that actively transport ions. In t...

    journal_title：Journal of structural biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jsb.2007.01.011

    authors： Screpanti E,Hunte C

    更新日期：2007-08-01 00:00:00

  • Spectroscopy on the wing: Investigating possible differences in protein secondary structures in feather shafts of birds using Raman spectroscopy.

    abstract：:The central shaft of a bird's flight feather bears most of the aerodynamic load during flight and exhibits some remarkable mechanical properties. The shaft comprises two parts, the calamus and the rachis. The calamus is at the base of the shaft, while the rachis is the longer upper part which supports the vanes. The s...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2020.107529

    authors： Laurent CM,Dyke JM,Cook RB,Dyke G,de Kat R

    更新日期：2020-07-01 00:00:00

  • Conical Fourier shell correlation applied to electron tomograms.

    abstract：:The resolution of electron tomograms is anisotropic due to geometrical constraints during data collection, such as the limited tilt range and single axis tilt series acquisition. Acquisition of dual axis tilt series can decrease these effects. However, in cryo-electron tomography, to limit the electron radiation damag...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2015.03.010

    authors： Diebolder CA,Faas FG,Koster AJ,Koning RI

    更新日期：2015-05-01 00:00:00

  • Intracellular calcium phosphate deposits contribute to transcellular calcium transport within the hepatopancreas of Porcellio scaber.

    abstract：:Like in most Crustacea, the cuticle of terrestrial isopods is hardened by a calcareous mineral phase. This rigid cuticle is frequently shed during a process called moulting. To reduce calcium loss, Porcellio scaber eats the shed cuticle, the exuviae, and absorb the calcium from it through large tubular diverticula of ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2020.107613

    authors： Rupp U,Walther P,Ziegler A

    更新日期：2020-11-01 00:00:00

  • Three-dimensional reconstruction with contrast transfer function correction from energy-filtered cryoelectron micrographs: procedure and application to the 70S Escherichia coli ribosome.

    abstract：:Cryoelectron microscopy provides the means of studying macromolecules in their native state. However, the contrast transfer function (CTF) makes the images and the three-dimensional (3D) maps derived from them difficult to interpret. We developed methods to determine the CTF from experimental data and to obtain a CTF-...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1997.3845

    authors： Zhu J,Penczek PA,Schröder R,Frank J

    更新日期：1997-04-01 00:00:00

  • Adaptations of a tropical swamp worm, alma emini, for subsistence in a H2S-rich habitat: evolution of endosymbiotic bacteria, sulfide metabolizing bodies, and novel processes of elimination of neutralized sulfide complexes

    abstract：:The epithelial cell lining of the respiratory groove of Alma emini, an oligochaete glossoscolecid worm that lives in a hydrogen sulfide (H2S)-rich tropical swamp, was investigated by transmission electron microscopy to determine the underlying structural adaptations which enable the worm to subsist in a highly inimica...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1998.4000

    authors： Maina JN,Maloiy GMO

    更新日期：1998-01-01 00:00:00

  • Morphological changes of the nucleolus during oogenesis in oviparous teleost fish, Barbus barbus (L.).

    abstract：:In fishes, like in amphibians, it is well established that variations in rRNA activity occur during oogenesis. Contrary to amphibians, however, little is known about the ultrastructural changes of the nucleolus during fish oogenesis. Evolution of the nucleolus has been followed during oogenesis in the teleost fish Bar...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2005.07.006

    authors： Thiry M,Poncin P

    更新日期：2005-10-01 00:00:00

  • Ultrastructural analysis of bacteriophage Φ29 during infection of Bacillus subtilis.

    abstract：:Recent advances in cryo-electron tomography (cryo-ET) have allowed direct visualization of the initial interactions between bacteriophages and their hosts. Previous studies focused on phage infection in Gram-negative bacteria but it is of particular interest how phages penetrate the thick, highly cross-linked Gram-pos...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2016.07.019

    authors： Farley MM,Tu J,Kearns DB,Molineux IJ,Liu J

    更新日期：2017-02-01 00:00:00

  • Crystal structure of thebaine 6-O-demethylase from the morphine biosynthesis pathway.

    abstract：:Thebaine 6-O-demethylase (T6ODM) from Papaver somniferum (opium poppy), which belongs to the non-heme 2-oxoglutarate/Fe(II)-dependent dioxygenases (ODD) family, is a key enzyme in the morphine biosynthesis pathway. Initially, T6ODM was characterized as an enzyme catalyzing O-demethylation of thebaine to neopinone and ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.01.007

    authors： Kluza A,Niedzialkowska E,Kurpiewska K,Wojdyla Z,Quesne M,Kot E,Porebski PJ,Borowski T

    更新日期：2018-06-01 00:00:00

  • Structure of a hexameric RNA packaging motor in a viral polymerase complex.

    abstract：:Packaging of the Cystovirus varphi8 genome into the polymerase complex is catalysed by the hexameric P4 packaging motor. The motor is located at the fivefold vertices of the icosahedrally symmetric polymerase complex, and the symmetry mismatch between them may be critical for function. We have developed a novel image-...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2006.08.021

    authors： Huiskonen JT,Jäälinoja HT,Briggs JA,Fuller SD,Butcher SJ

    更新日期：2007-05-01 00:00:00

  • Zernike phase contrast cryo-electron tomography.

    abstract：:Cryo-tomography in the electron microscope is unique in its ability to provide high-resolution, three-dimensional structural information about cells, organelles and macromolecules in a nearly native, frozen-hydrated state. However, the phase-contrast imaging method used in conventional cryo-electron tomography fails t...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2010.03.013

    authors： Danev R,Kanamaru S,Marko M,Nagayama K

    更新日期：2010-08-01 00:00:00

  • Identification and crystallization of a protease-resistant core of calnexin that retains biological activity.

    abstract：:Calnexin is a molecular chaperone that facilitates folding of glycoproteins in the endoplasmic reticulum (ER). The cloned lumenal domain of canine calnexin, cnxDeltaTMC, retains its biological activity without the transmembrane and cytosolic region. For the purpose of structure determination we generated a crystalliza...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1998.4032

    authors： Hahn M,Borisova S,Schrag JD,Tessier DC,Zapun A,Tom R,Kamen AA,Bergeron JJ,Thomas DY,Cygler M

    更新日期：1998-11-01 00:00:00

  • Crystallization and preliminary X-ray diffraction analysis of nuclear transport factor 2.

    abstract：:We have cloned and expressed in Escherichia coli cDNA for rat nuclear transport factor 2 (NTF2), a key cytoplasmic factor that facilitates the import of proteins into the nucleus through nuclear pores. We have used this bacterially expressed material to produce orthorhombic crystals suitable for high-resolution X-ray ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1996.0049

    authors： Kent HM,Clarkson WD,Bullock TL,Stewart M

    更新日期：1996-03-01 00:00:00

  • The structure of the contractile apparatus in ultrarapidly frozen smooth muscle: freeze-fracture, deep-etch, and freeze-substitution studies.

    abstract：:The structure of the smooth muscle contractile apparatus was studied using ultrarapid freezing followed by freeze substitution or by longitudinal freeze-fracture, deep-etch, and platinum-carbon replication. Freeze substitution minimises the detrimental effects of chemical fixation and freeze fracture eliminates them e...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1995.1009

    authors： Hodgkinson JL,Newman TM,Marston SB,Severs NJ

    更新日期：1995-03-01 00:00:00

  • Topological mapping of 13 epitopes on a subunit of Androctonus australis hemocyanin.

    abstract：:A topological localization of epitopes on the surface of the Aa6 subunit of Androctonus australis hemocyanin has been carried out. First, immunocomplex strings composed of native hemocyanin and monoclonal antibodies were examined in the electron microscope and submitted to an image processing by correspondence analysi...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/1047-8477(90)90087-s

    authors： Lamy J,Billiald P,Taveau JC,Boisset N,Motta G,Lamy J

    更新日期：1990-03-01 00:00:00

  • Purification, crystallization, and preliminary X-ray analysis of L-A: a dsRNA yeast virus.

    abstract：:TheL-A virus (LAV) particle is a specialized compartment for the transcription and replication of double-stranded RNA. It is 390 A in diameter and infects yeast. The particle is formed by a capsid containing 120 copies of a 680-residue gene product arranged with T = 1 icosahedral symmetry, approximately two copies of ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.2001.4371

    authors： Naitow H,Canady MA,Lin T,Wickner RB,Johnson JE

    更新日期：2001-07-01 00:00:00

  • Optimized cryo-focused ion beam sample preparation aimed at in situ structural studies of membrane proteins.

    abstract：:While cryo-electron tomography (cryo-ET) can reveal biological structures in their native state within the cellular environment, it requires the production of high-quality frozen-hydrated sections that are thinner than 300nm. Sample requirements are even more stringent for the visualization of membrane-bound protein c...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2016.07.010

    authors： Schaffer M,Mahamid J,Engel BD,Laugks T,Baumeister W,Plitzko JM

    更新日期：2017-02-01 00:00:00