Abstract:
:The emergence of electron tomography as a tool for three dimensional structure determination of cells and tissues has brought its own challenges for the preparation of thick sections. High pressure freezing in combination with freeze substitution provides the best method for obtaining the largest volume of well-preserved tissue. However, for deeply embedded, heterogeneous, labile tissues needing careful dissection, such as brain, the damage due to anoxia and excision before cryofixation is significant. We previously demonstrated that chemical fixation prior to high pressure freezing preserves fragile tissues and produces superior tomographic reconstructions compared to equivalent tissue preserved by chemical fixation alone. Here, we provide further characterization of the technique, comparing the ultrastructure of Flock House Virus infected DL1 insect cells that were (1) high pressure frozen without fixation, (2) high pressure frozen following fixation, and (3) conventionally prepared with aldehyde fixatives. Aldehyde fixation prior to freezing produces ultrastructural preservation superior to that obtained through chemical fixation alone that is close to that obtained when cells are fast frozen without fixation. We demonstrate using a variety of nervous system tissues, including neurons that were injected with a fluorescent dye and then photooxidized, that this technique provides excellent preservation compared to chemical fixation alone and can be extended to selectively stained material where cryofixation is impractical.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Sosinsky GE,Crum J,Jones YZ,Lanman J,Smarr B,Terada M,Martone ME,Deerinck TJ,Johnson JE,Ellisman MHdoi
10.1016/j.jsb.2007.09.002subject
Has Abstractpub_date
2008-03-01 00:00:00pages
359-71issue
3eissn
1047-8477issn
1095-8657pii
S1047-8477(07)00220-1journal_volume
161pub_type
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