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Pores of the toxin FraC assemble into 2D hexagonal clusters in both crystal structures and model membranes.

Abstract：

:The recent high-resolution structure of the toxin FraC derived from the sea anemone Actinia fragacea has provided new insight into the mechanism of pore formation by actinoporins. In this work, we report two new crystal forms of FraC in its oligomeric prepore conformation. Together with the previously reported structure, these two new structures reveal that ring-like nonamers of the toxin assemble into compact two-dimensional hexagonal arrays. This supramolecular organization is maintained in different relative orientations adopted by the oligomers within the crystal layers. Analyses of the aggregation of FraC pores in both planar and curved (vesicles) model membranes show similar 2D hexagonal arrangements. Our observations support a model in which hexagonal pore-packing is a clustering mechanism that maximizes toxin-driven membrane damage in the target cell.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Mechaly AE,Bellomio A,Morante K,Agirre J,Gil-Cartón D,Valle M,González-Mañas JM,Guérin DM

doi

10.1016/j.jsb.2012.06.003

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

312-7

issue

2

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(12)00183-9

journal_volume

180

pub_type

杂志文章

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