Abstract:
:Thebaine 6-O-demethylase (T6ODM) from Papaver somniferum (opium poppy), which belongs to the non-heme 2-oxoglutarate/Fe(II)-dependent dioxygenases (ODD) family, is a key enzyme in the morphine biosynthesis pathway. Initially, T6ODM was characterized as an enzyme catalyzing O-demethylation of thebaine to neopinone and oripavine to morphinone. However, the substrate range of T6ODM was recently expanded to a number of various benzylisoquinoline alkaloids. Here, we present crystal structures of T6ODM in complexes with 2-oxoglutarate (T6ODM:2OG, PDB: 5O9W) and succinate (T6ODM:SIN, PDB: 5O7Y). Both metal and 2OG binding sites display similarity to other proteins from the ODD family, but T6ODM is characterized by an exceptionally large substrate binding cavity, whose volume can partially explain the promiscuity of this enzyme. Moreover, the size of the cavity allows for binding of multiple molecules at once, posing a question about the substrate-driven specificity of the enzyme.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Kluza A,Niedzialkowska E,Kurpiewska K,Wojdyla Z,Quesne M,Kot E,Porebski PJ,Borowski Tdoi
10.1016/j.jsb.2018.01.007subject
Has Abstractpub_date
2018-06-01 00:00:00pages
229-235issue
3eissn
1047-8477issn
1095-8657pii
S1047-8477(18)30022-4journal_volume
202pub_type
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