Abstract：
:A topological localization of epitopes on the surface of the Aa6 subunit of Androctonus australis hemocyanin has been carried out. First, immunocomplex strings composed of native hemocyanin and monoclonal antibodies were examined in the electron microscope and submitted to an image processing by correspondence analysis. The average images were then compared to a three-dimensional model of the 24-mer suggesting that 11 of the 13 epitopes are located in three zones of the subunit surface. Second, the overlaps between the epitopes were then studied by polyacrylamide gel electrophoresis, competitive binding inhibition, and immunoelectron microscopy. Four groups of epitopes were identified. One group was capable of binding exclusively to the free subunit. The other three groups were identical to those found in immunoelectron microscopy. The data are consistent with the existence of a small number of immunodominant regions on the surface of the Aa6 subunit.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Lamy J,Billiald P,Taveau JC,Boisset N,Motta G,Lamy Jdoi
10.1016/1047-8477(90)90087-ssubject
Has Abstractpub_date
1990-03-01 00:00:00pages
64-74issue
1eissn
1047-8477issn
1095-8657journal_volume
103pub_type
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