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Denoising of high-resolution single-particle electron-microscopy density maps by their approximation using three-dimensional Gaussian functions.

Abstract：

:Cryo-electron microscopy (cryo-EM) of frozen-hydrated preparations of isolated macromolecular complexes is the method of choice to obtain the structure of complexes that cannot be easily studied by other experimental methods due to their flexibility or large size. An increasing number of macromolecular structures are currently being obtained at subnanometer resolution but the interpretation of structural details in such EM-derived maps is often difficult because of noise at these high-frequency signal components that reduces their contrast. In this paper, we show that the method for EM density-map approximation using Gaussian functions can be used for denoising of single-particle EM maps of high (typically subnanometer) resolution. We show its denoising performance using simulated and experimental EM density maps of several complexes.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Jonić S,Vargas J,Melero R,Gómez-Blanco J,Carazo JM,Sorzano CO

doi

10.1016/j.jsb.2016.04.007

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

423-33

issue

3

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(16)30066-1

journal_volume

194

pub_type

杂志文章

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