Abstract：
:Hedgehog signaling plays a crucial role in embryogenesis and adult tissue homeostasis, and mutations of its key components such as Suppressor of fused (Sufu) are closely associated with human diseases. The Ser/Thr kinase Fused (Fu) promotes Hedgehog signaling by phosphorylating the Cubitus interruptus (Ci)/Glioma-associated oncogene homologue (Gli) family of transcription factors. Sufu associates with both Fu and Ci/Gli, but the recognition mechanism between Sufu and Fu remains obscure. Here, our structure of the N-terminal domain (NTD) of Drosophila Sufu (dSufu) in complex with the Sufu-binding site (SBS) of Fu reveals that both main-chain β sheet formation and side-chain hydrophobic interactions contribute to the recognition between Sufu and Fu, and point mutations of highly conserved interface residues eliminated their association. Structural comparison suggests that Fu and Ci/Gli bind on opposite sides of dSufu-NTD, allowing the formation of a Fu-dSufu-Ci ternary complex which facilitates the phosphorylation of Ci/Gli by Fu. Hence, our results provide insights into the Sufu-Fu recognition mechanism.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Li H,Wang W,Zhang W,Wu Gdoi
10.1016/j.jsb.2020.107614subject
Has Abstractpub_date
2020-11-01 00:00:00pages
107614issue
2eissn
1047-8477issn
1095-8657pii
S1047-8477(20)30187-8journal_volume
212pub_type
杂志文章abstract：:Studying nanostructured hierarchical materials such as the biomineralized bone is challenging due to their complex 3D structures that call for high spatial resolution. One route to study such materials is X-ray powder diffraction computed tomography (XRD-CT) that reveals the 3D distribution of crystalline phases and X...
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journal_title：Journal of structural biology
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2009.02.007
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.1999.4106
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journal_title：Journal of structural biology
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doi：10.1016/j.jsb.2016.03.002
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journal_title：Journal of structural biology
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journal_title：Journal of structural biology
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abstract：:The Dugesia japonica vasa-like gene B (DjVLGB) protein is a DEAD-box RNA helicase of a planarian, which is well known for its strong regenerative capacity. DjVLGB shares sequence similarity to the Drosophila germ-line-specific DEAD-box RNA helicase Vasa, and even higher similarity to its paralogue, mouse PL10. In this...
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journal_title：Journal of structural biology
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journal_title：Journal of structural biology
pub_type： 杂志文章,评审
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journal_title：Journal of structural biology
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.1996.0071
更新日期：1996-07-01 00:00:00
abstract：:HIV-1 Rev mediates the nuclear export of unspliced and partially-spliced viral transcripts for the production of progeny genomes and structural proteins. In this process, four (or more) copies of Rev assemble onto a highly-structured 351-nt region in such viral transcripts, the Rev response element (RRE). How this occ...
journal_title：Journal of structural biology
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doi：10.1016/j.jsb.2018.03.011
更新日期：2018-08-01 00:00:00
