Abstract:
:We consider the problem of estimating an unbiased and reference-free ab initio model for non-symmetric molecules from images generated by single-particle cryo-electron microscopy. The proposed algorithm finds the globally optimal assignment of orientations that simultaneously respects all common lines between all images. The contribution of each common line to the estimated orientations is weighted according to a statistical model for common lines' detection errors. The key property of the proposed algorithm is that it finds the global optimum for the orientations given the common lines. In particular, any local optima in the common lines energy landscape do not affect the proposed algorithm. As a result, it is applicable to thousands of images at once, very robust to noise, completely reference free, and not biased towards any initial model. A byproduct of the algorithm is a set of measures that allow to asses the reliability of the obtained ab initio model. We demonstrate the algorithm using class averages from two experimental data sets, resulting in ab initio models with resolutions of 20Å or better, even from class averages consisting of as few as three raw images per class.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Greenberg I,Shkolnisky Ydoi
10.1016/j.jsb.2017.09.007subject
Has Abstractpub_date
2017-11-01 00:00:00pages
106-117issue
2eissn
1047-8477issn
1095-8657pii
S1047-8477(17)30158-2journal_volume
200pub_type
杂志文章abstract::Tricorn protease from the archaeon Thermoplasma acidophilum acts "downstream" of the proteasome; in conjunction with its aminopeptidase cofactors it converts peptides generated by the proteasome into free amino acids. The basic functional unit of Tricorn is a homohexamer of the 121-kDa subunit, 20 of which can assembl...
journal_title:Journal of structural biology
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journal_title:Journal of structural biology
pub_type: 杂志文章
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journal_title:Journal of structural biology
pub_type: 杂志文章
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journal_title:Journal of structural biology
pub_type: 杂志文章
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journal_title:Journal of structural biology
pub_type: 杂志文章
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2011.11.019
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journal_title:Journal of structural biology
pub_type: 杂志文章
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/s1047-8477(02)00020-5
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pub_type: 杂志文章
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journal_title:Journal of structural biology
pub_type: 杂志文章
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1006/jsbi.1995.1044
更新日期:1995-09-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2014.03.022
更新日期:2014-06-01 00:00:00
abstract::Studying nanostructured hierarchical materials such as the biomineralized bone is challenging due to their complex 3D structures that call for high spatial resolution. One route to study such materials is X-ray powder diffraction computed tomography (XRD-CT) that reveals the 3D distribution of crystalline phases and X...
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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abstract::HIV-1 Rev mediates the nuclear export of unspliced and partially-spliced viral transcripts for the production of progeny genomes and structural proteins. In this process, four (or more) copies of Rev assemble onto a highly-structured 351-nt region in such viral transcripts, the Rev response element (RRE). How this occ...
journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2018.03.011
更新日期:2018-08-01 00:00:00