当前位置： SCI文献检索 > JOURNAL OF STRUCTURAL BIOLOGY期刊下所有文献 > Unliganded and CMP-Neu5Ac bound structures of human α-2,6-sialyltransferase ST6Gal I at high resolution.

Unliganded and CMP-Neu5Ac bound structures of human α-2,6-sialyltransferase ST6Gal I at high resolution.

Abstract：

:Sialic acid residues found as terminal monosaccharides in various types of glycan chains in cell surface glycoproteins and glycolipids have been identified as important contributors of cell-cell interactions in normal vs. abnormal cellular behavior and are pivotal in diseases such as cancers. In vertebrates, sialic acids are attached to glycan chains by a conserved subset of sialyltransferases with different enzymatic and substrate specificities. ST6Gal I is a sialyltransferase using activated CMP-sialic acids as donor substrates to catalyze the formation of a α2,6-glycosidic bond between the sialic acid residue and the acceptor disaccharide LacNAc. Understanding sialyltransferases at the molecular and structural level shed light into their function. We present here two human ST6Gal I structures, which show for the first time the enzyme in the unliganded state and with the full donor substrate CMP-Neu5Ac bound. Comparison of these structures reveal flexibility of the catalytic loop, since in the unliganded structure Tyr354 adopts a conformation seen also as an alternate conformation in the substrate bound structure. CMP-Neu5Ac is bound with the side chain at C5 of the sugar residue directed outwards at the surface of the protein. Furthermore, the exact binding mode of the sialic acid moiety of the substrate directly involves sialylmotifs L, S and III and positions the sialylmotif VS in the immediate vicinity. We also present a model for the ternary complex of ST6Gal I with both the donor and the acceptor substrates.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Harrus D,Harduin-Lepers A,Glumoff T

doi

10.1016/j.jsb.2020.107628

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

107628

issue

2

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(20)30201-X

journal_volume

212

pub_type

杂志文章

相关文献

JOURNAL OF STRUCTURAL BIOLOGY文献大全
  • Crystal structures and putative interface of Saccharomyces cerevisiae mitochondrial matrix proteins Mmf1 and Mam33.

    abstract：:The yeast Saccharomyces cerevisiae mitochondrial matrix factor Mmf1, a member in the YER057c/Yigf/Uk114 family, participates in isoleucine biosynthesis and mitochondria maintenance. Mmf1 physically interacts with another mitochondrial matrix protein Mam33, which is involved in the sorting of cytochrome b₂ to the inter...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2011.05.005

    authors： Pu YG,Jiang YL,Ye XD,Ma XX,Guo PC,Lian FM,Teng YB,Chen Y,Zhou CZ

    更新日期：2011-09-01 00:00:00

  • A strategy for electron tomographic data collection and crystallographic reconstruction of biological bundles.

    abstract：:Structures of highly ordered biological bundles have unique features which call for special experimental and computational methods in electron cryomicroscopy. They can be considered as three-dimensional quasi-crystals and reconstructed using a crystallographic approach. However, they are neither "infinitely" large wit...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1997.3916

    authors： Sherman MB,Jakana J,Sun S,Matsudaira P,Chiu W,Schmid MF

    更新日期：1997-12-01 00:00:00

  • Identification of the tailspike protein from the Salmonella newington phage epsilon 34 and partial characterization of its phage-associated properties.

    abstract：:The lipopolysaccharide (LPS) of the Salmonella cell surface serves as the receptor for a very large number of bacterial viruses. The tailspike protein from these viruses recognizes the LPS as its initial receptor. It is proposed that the study of the P22 and epsilon 34 tailspike proteins could serve as a model for the...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1995.1053

    authors： Greenberg M,Dunlap J,Villafane R

    更新日期：1995-11-01 00:00:00

  • Copper ions trigger disassembly of neurokinin B functional amyloid and inhibit de novo assembly.

    abstract：:The formation of amyloid is considered an intrinsic ability of most polypeptides. It is a structure adopted by many neuropeptides and neurohormones during the formation of dense core vesicles in secretory cells, yet the mechanisms mediating assembly and disassembly of these amyloids remain unclear. Neurokinin B is a n...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2019.09.011

    authors： Jayawardena BM,Jones MR,Hong Y,Jones CE

    更新日期：2019-12-01 00:00:00

  • Vectorization with SIMD extensions speeds up reconstruction in electron tomography.

    abstract：:Electron tomography allows structural studies of cellular structures at molecular detail. Large 3D reconstructions are needed to meet the resolution requirements. The processing time to compute these large volumes may be considerable and so, high performance computing techniques have been used traditionally. This work...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2010.01.008

    authors： Agulleiro JI,Garzón EM,García I,Fernández JJ

    更新日期：2010-06-01 00:00:00

  • Visualization of poly(A)-binding protein complex formation with poly(A) RNA using atomic force microscopy.

    abstract：:Poly(A)-binding protein (PABP) is an RNA-binding protein that binds specifically to the poly(A) tail of messenger RNAs in eukaryotes. The PABP/poly(A) tail complex has been implicated as being important in promoting the efficient initiation of translation as well as in maintaining the integrity of the mRNA. PABP binds...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1997.3864

    authors： Smith BL,Gallie DR,Le H,Hansma PK

    更新日期：1997-07-01 00:00:00

  • Axially aligned organic fibers and amorphous calcium phosphate form the claws of a terrestrial isopod (Crustacea).

    abstract：:Skeletal elements that are exposed to heavy mechanical loads may provide important insights into the evolutionary solutions to mechanical challenges. We analyzed the microscopic architecture of dactylus claws in the woodlice Porcellio scaber and correlated these observations with analyses of the claws' mineral composi...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2016.06.008

    authors： Vittori M,Srot V,Žagar K,Bussmann B,van Aken PA,Čeh M,Štrus J

    更新日期：2016-08-01 00:00:00

  • Focal pair merging for contrast enhancement of single particles.

    abstract：:A common technique in transmission electron microscopy is the collection of a focal pair, in which the first, close to focus image contains higher resolution information at lower contrast, and the second, far from focus image has high contrast but less reliable high-resolution information. Typically these second micro...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2003.10.012

    authors： Ludtke SJ,Chiu W

    更新日期：2003-10-01 00:00:00

  • 3-dimensional imaging of collagen using second harmonic generation.

    abstract：:Collagen is the most important structural protein of the animal body. Its unique triple-helix structure and extremely high level of crystallinity make it exceptionally efficient in generating the second harmonic of incident light, and we show here how this leads to a novel mode of microscopy of immediate practical sig...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/s1047-8477(02)00576-2

    authors： Cox G,Kable E,Jones A,Fraser I,Manconi F,Gorrell MD

    更新日期：2003-01-01 00:00:00

  • Ultrastructural characterization of RPA-containing domains in nuclei assembled in Xenopus egg extracts.

    abstract：:We describe novel structural domains in in vitro reconstituted Xenopus sperm nuclei, which we term RPA bodies; RPA is the only known marker of these structures. These bodies contain DNA and represent special chromatin domains as seen by transmission electron microscopy. We show that RPA bodies exhibit a similar ultras...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.2000.4229

    authors： Eltsov M,Grandi P,Raska I

    更新日期：2000-04-01 00:00:00

  • Structure of dihydropyrimidinase from Sinorhizobium meliloti CECT4114: new features in an amidohydrolase family member.

    abstract：:The recombinant dihydropyrimidinase from Sinorhizobium meliloti CECT4114 (SmelDhp) has been characterised and its crystal structure elucidated at 1.85A. The global architecture of the protein is reminiscent of that of the amidohydrolase superfamily, consisting of two domains; an (alpha/beta)(8) TIM-like barrel domain,...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.10.013

    authors： Martínez-Rodríguez S,Martínez-Gómez AI,Clemente-Jiménez JM,Rodríguez-Vico F,García-Ruíz JM,Las Heras-Vázquez FJ,Gavira JA

    更新日期：2010-02-01 00:00:00

  • In situ detection of specific gene expression during and immediately after transcription at electron microscopic level.

    abstract：:In situ hybridization (ISH) is a widely applied technique used for visualizing specific nucleic acid sequences at chromosomal, cytologic, and histologic levels. It sometimes fails, however, to demonstrate precise cell identity, early stages of gene expression and variants of alternative splicing because of its limited...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2005.09.010

    authors： Kitazawa S,Kitazawa R

    更新日期：2006-01-01 00:00:00

  • The structure of a COPII tubule.

    abstract：:Nearly a third of all eukaryotic proteins are transported from the ER to the Golgi apparatus through the secretory pathway using COPII coated vesicles. Evidence suggests that this transport occurs via 500-900 Å vesicles that bud from the ER membrane. It has been shown that procollagen molecules utilize the COPII prote...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2010.09.002

    authors： O'Donnell J,Maddox K,Stagg S

    更新日期：2011-02-01 00:00:00

  • Synchrotron microComputed Tomography of the mature bovine dentinoenamel junction.

    abstract：:The mature dentinoenamel junction (DEJ) is viewed by some investigators and the current authors, not as a fossilized, sharp transition between enamel and dentin, but as a relatively broad structural transition zone including the mantle dentin and the inner aprismatic enamel. In this study, the DEJ structure in bovine ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2007.10.006

    authors： Stock SR,Vieira AE,Delbem AC,Cannon ML,Xiao X,Carlo FD

    更新日期：2008-02-01 00:00:00

  • The structure of the contractile apparatus in ultrarapidly frozen smooth muscle: freeze-fracture, deep-etch, and freeze-substitution studies.

    abstract：:The structure of the smooth muscle contractile apparatus was studied using ultrarapid freezing followed by freeze substitution or by longitudinal freeze-fracture, deep-etch, and platinum-carbon replication. Freeze substitution minimises the detrimental effects of chemical fixation and freeze fracture eliminates them e...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1995.1009

    authors： Hodgkinson JL,Newman TM,Marston SB,Severs NJ

    更新日期：1995-03-01 00:00:00

  • Strategies for the crystallization of viruses: using phase diagrams and gels to produce 3D crystals of Grapevine fanleaf virus.

    abstract：:The small icosahedral plant RNA nepovirus Grapevine fanleaf virus (GFLV) is specifically transmitted by a nematode and causes major damage to vineyards worldwide. To elucidate the molecular mechanisms underlying the recognition between the surface of its protein capsid and cellular components of its vector, host and v...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2011.02.007

    authors： Schellenberger P,Demangeat G,Lemaire O,Ritzenthaler C,Bergdoll M,Oliéric V,Sauter C,Lorber B

    更新日期：2011-05-01 00:00:00

  • Rapid near-atomic resolution single-particle 3D reconstruction with SIMPLE.

    abstract：:Cryogenic electron microscopy (cryo-EM) and single-particle analysis enables determination of near-atomic resolution structures of biological molecules. However, large computational requirements limit throughput and rapid testing of new image processing tools. We developed PRIME, an algorithm part of the SIMPLE softwa...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.08.005

    authors： Reboul CF,Kiesewetter S,Eager M,Belousoff M,Cui T,De Sterck H,Elmlund D,Elmlund H

    更新日期：2018-11-01 00:00:00

  • Molecular mechanism for the action of the anti-CD44 monoclonal antibody MEM-85.

    abstract：:The hyaluronate receptor CD44 plays role in cell adhesion and migration and is involved in tumor metastasis. The extracellular domain of CD44 comprises the hyaluronate-binding domain (HABD) and the membrane-proximal stem region; the short intracellular portion interacts with adaptor proteins and triggers signaling pat...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2015.06.005

    authors： Škerlová J,Král V,Kachala M,Fábry M,Bumba L,Svergun DI,Tošner Z,Veverka V,Řezáčová P

    更新日期：2015-08-01 00:00:00

  • Structural characteristics of the beta-sheet-like human and rat islet amyloid polypeptides as determined by scanning tunneling microscopy.

    abstract：:We demonstrate in this work that scanning tunneling microscopy (STM) provides a useful approach to obtaining structural information about human islet amyloid polypeptide (hIAPP) and rat islet amyloid polypeptide (rIAPP) assembly on highly oriented pyrolytic graphite (HOPG) with sub-molecular resolution. The observed h...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.05.009

    authors： Mao X,Ma X,Liu L,Niu L,Yang Y,Wang C

    更新日期：2009-09-01 00:00:00

  • Cellular electron cryo tomography and in situ sub-volume averaging reveal the context of microtubule-based processes.

    abstract：:Electron cryo-tomography (cryoET) is currently the only technique that allows the direct observation of proteins in their native cellular environment. Sub-volume averaging of electron tomograms offers a route to increase the signal-to-noise of repetitive biological structures, such improving the information content an...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2016.06.024

    authors： Grange M,Vasishtan D,Grünewald K

    更新日期：2017-02-01 00:00:00

  • Cep120 promotes microtubule formation through a unique tubulin binding C2 domain.

    abstract：:Centrioles are microtubule-based structures that play essential roles in cell division and cilia biogenesis. Cep120 is an important protein for correct centriole formation and mutations in the Cep120 gene cause severe human diseases like Joubert syndrome and complex ciliopathies. Here, we show that Cep120 contains thr...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.01.009

    authors： Sharma A,Gerard SF,Olieric N,Steinmetz MO

    更新日期：2018-07-01 00:00:00

  • Tooth movements are guided by specific contact areas between the tooth root and the jaw bone: A dynamic 3D microCT study of the rat molar.

    abstract：:Teeth sustain high loads over a lifetime and yet intact tooth failure is rare. The different structures of the tooth, jaw bone and the intervening soft periodontal ligament enable the tooth to endure repeated loading during mastication. Although mechanical and functional properties of the different components are thor...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2011.11.019

    authors： Naveh GR,Shahar R,Brumfeld V,Weiner S

    更新日期：2012-02-01 00:00:00

  • Collagen matrix remodeling in 3-dimensional cellular space resolved using second harmonic generation and multiphoton excitation fluorescence.

    abstract：:The structural remodeling of collagen is important in biological processes such as fibrosis, developmental morphogenesis and wound repair. Highly ordered collagen macromolecules produce second harmonic generation signals without the need for any exogenous label. Conversely, the cellular components stained with exogeno...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.07.023

    authors： Abraham T,Carthy J,McManus B

    更新日期：2010-01-01 00:00:00

  • Determination of astigmatism in TEM images.

    abstract：:We have developed a new two-step algorithm to determine the astigmatism of images from transmission electron microscopes (TEMs). Instead of computing the radial average of the power spectrum, we divide the power spectrum of a TEM image 1 to m (typically 32) sectors. We use a technique based on perturbation analysis of...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2006.07.021

    authors： Fernando KV,Fuller SD

    更新日期：2007-01-01 00:00:00

  • Accurate modeling of single-particle cryo-EM images quantitates the benefits expected from using Zernike phase contrast.

    abstract：:The use of a Zernike-type phase plate in biologic cryo-electron microscopy allows the imaging, without using defocus, of what are predominantly phase objects. It is thought that such phase-plate implementations might result in higher quality images, free from the problems of CTF correction that occur when images must ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2011.03.020

    authors： Hall RJ,Nogales E,Glaeser RM

    更新日期：2011-06-01 00:00:00

  • Combined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification.

    abstract：:Hard X-ray fluorescence microscopy and magnified phase contrast imaging are combined to obtain quantitative maps of the projected metal concentration in whole cells. The experiments were performed on freeze dried cells at the nano-imaging station ID22NI of the European Synchrotron Radiation Facility (ESRF). X-ray fluo...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2011.12.005

    authors： Kosior E,Bohic S,Suhonen H,Ortega R,Devès G,Carmona A,Marchi F,Guillet JF,Cloetens P

    更新日期：2012-02-01 00:00:00

  • Local analysis of strains and rotations for macromolecular electron microscopy maps.

    abstract：:Macromolecular complexes perform their physiological functions by local rearrangements of their constituents and biochemically interacting with their reaction partners. These rearrangements may involve local rotations and the induction of local strains causing different mechanical efforts and stretches at the differen...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2016.04.001

    authors： Sorzano CO,Martín-Ramos A,Prieto F,Melero R,Martín-Benito J,Jonic S,Navas-Calvente J,Vargas J,Otón J,Abrishami V,de la Rosa-Trevín JM,Gómez-Blanco J,Vilas JL,Marabini R,Carazo JM

    更新日期：2016-07-01 00:00:00

  • Nuclear lamina at the crossroads of the cytoplasm and nucleus.

    abstract：:The nuclear lamina is a protein meshwork that lines the nuclear envelope in metazoan cells. It is composed largely of a polymeric assembly of lamins, which comprise a distinct sequence homology class of the intermediate filament protein family. On the basis of its structural properties, the lamina originally was propo...

    journal_title：Journal of structural biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jsb.2011.11.007

    authors： Gerace L,Huber MD

    更新日期：2012-01-01 00:00:00

  • Flight muscle-specific Pro-Ala-rich extension of troponin is important for maintaining the insect-type myofilament lattice integrity.

    abstract：:Insect flight muscle (IFM) can oscillate at frequencies up to 1000Hz, owing to its capability of stretch activation (SA). It is a highly specialized form of cross striated muscles, and its peculiar features include the IFM-specific isoform of troponin-I (troponin-H or TnH) with an unusually long Pro-Ala-rich extension...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2013.05.009

    authors： Iwamoto H

    更新日期：2013-07-01 00:00:00

  • Sequence analyses of Type I and Type II chains in human hair and epithelial keratin intermediate filaments: promiscuous obligate heterodimers, Type II template for molecule formation and a rationale for heterodimer formation.

    abstract：:Sequence comparisons have been undertaken for all hair and epithelial keratin IF chains from a single species--human. The results lead to several new proposals. First, it is clear that not only is the chain structure of the molecule an obligate heterodimer but promiscuous association of Type I and Type II chains must ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2006.12.002

    authors： Smith TA,Parry DA

    更新日期：2007-06-01 00:00:00