Abstract:
:Poly(A)-binding protein (PABP) is an RNA-binding protein that binds specifically to the poly(A) tail of messenger RNAs in eukaryotes. The PABP/poly(A) tail complex has been implicated as being important in promoting the efficient initiation of translation as well as in maintaining the integrity of the mRNA. PABP binds poly(A) cooperatively with a packing density of one PABP molecule per 25 adenosine residues. We have investigated the complexes formed between purified PABP and poly(A) RNA using atomic force microscopy (AFM). PABP alone was observed to be primarily in a monomer form with a height of 1.0 +/- 0.2 nm. Following binding to poly(A), PABP appeared to be present in variable size complexes that bound lengthwise along the RNA. This size of the PABP/poly(A) complex appeared to be maximal, suggesting that PABP binding to poly(A) may be self-limiting. Poly(A) RNA alone appeared to contain a knob-like structure that largely disappeared once PABP was bound. The use of AFM has therefore provided potential new insights into the complexes formed by this RNA-binding protein.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Smith BL,Gallie DR,Le H,Hansma PKdoi
10.1006/jsbi.1997.3864subject
Has Abstractpub_date
1997-07-01 00:00:00pages
109-17issue
2eissn
1047-8477issn
1095-8657pii
S1047-8477(97)93864-8journal_volume
119pub_type
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journal_title:Journal of structural biology
pub_type: 杂志文章
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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更新日期:2015-12-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
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更新日期:2012-07-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
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journal_title:Journal of structural biology
pub_type: 杂志文章
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更新日期:2016-07-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
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更新日期:2013-07-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1006/jsbi.1997.3851
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journal_title:Journal of structural biology
pub_type: 杂志文章
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2009.06.006
更新日期:2009-09-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1006/jsbi.1998.4011
更新日期:1998-09-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2010.08.004
更新日期:2011-01-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2015.11.005
更新日期:2016-01-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/1047-8477(91)90075-8
更新日期:1991-06-01 00:00:00
abstract::In many microorganisms, menaquinone is an essential lipid-soluble electron carrier. Recently, an alternative menaquinone biosynthetic pathway was found in some microorganisms [Hiratsuka, T., Furihata, K., Ishikawa, J., Yamashita, H., Itoh, N., Seto, H., Dairi, T., 2008. An alternative menaquinone biosynthetic pathway ...
journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2009.07.007
更新日期:2009-12-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2007.09.003
更新日期:2007-12-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章,评审
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2008.07.006
更新日期:2008-10-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
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更新日期:2018-05-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2017.03.005
更新日期:2017-06-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2009.03.012
更新日期:2009-07-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1006/jsbi.2002.4458
更新日期:2002-01-01 00:00:00
abstract::Hitherto, the packing arrangement of the aquaporin-1 (AQP1) tetramer in 2-dimensional (2-D) crystals (two-sided plane group p42(1)2) was observed to be largely similar (canonical crystal form) despite the difference in the source of the protein, the glycosylation state of the protein, the type of lipids, and the ratio...
journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1006/jsbi.2000.4211
更新日期:2000-05-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2018.05.008
更新日期:2018-09-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2013.05.017
更新日期:2013-08-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2018.08.016
更新日期:2018-11-01 00:00:00
abstract::Zinc-induced sheets of tubulin are two-dimensional crystalline polymers that constitute an ideal sample for high resolution studies of tubulin by electron crystallography. We show that these 2-dimensional tubulin crystals can be stabilized by taxol against low-temperature depolymerization and degradation with time, ea...
journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1006/jsbi.1995.1044
更新日期:1995-09-01 00:00:00