Abstract：
:BCP1 is a protein enriched in the nucleus that is required for Mss4 nuclear export and identified as the chaperone of ribosomal protein Rpl23 in Saccharomyces cerevisiae. According to sequence homology, BCP1 is related to the mammalian BRCA2-interacting protein BCCIP and belongs to the BCIP protein family (PF13862) in the Pfam database. However, the BCIP family has no discernible similarity to proteins with known structure. Here, we report the crystal structure of BCP1, presenting an α/β fold in which the central antiparallel β-sheet is flanked by helices. Protein structural classification revealed that BCP1 has similarity to the GNAT superfamily but no conserved substrate-binding residues. Further modeling and protein-protein docking work provide a plausible model to explain the interaction between BCP1 and Rpl23. Our structural analysis presents the first structure of BCIP family and provides a foundation for understanding the molecular basis of BCP1 as a chaperone of Rpl23 for ribosome biosynthesis.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Lin MH,Kuo PC,Chiu YC,Chang YY,Chen SC,Hsu CHdoi
10.1016/j.jsb.2020.107605subject
Has Abstractpub_date
2020-10-01 00:00:00pages
107605issue
1eissn
1047-8477issn
1095-8657pii
S1047-8477(20)30178-7journal_volume
212pub_type
杂志文章abstract：:TheL-A virus (LAV) particle is a specialized compartment for the transcription and replication of double-stranded RNA. It is 390 A in diameter and infects yeast. The particle is formed by a capsid containing 120 copies of a 680-residue gene product arranged with T = 1 icosahedral symmetry, approximately two copies of ...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.2001.4371
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abstract：:The rod domain of IF molecules has been characterized as four alpha-helical coiled-coil segments (1A, 1B, 2A and 2B), three linkers (L1, L12 and L2) and a stutter at the centre of segment 2B. Two of these breaks in coiled-coil continuity (L2 and stutter) have been modelled on the basis of structural data obtained from...
journal_title：Journal of structural biology
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更新日期：2011-12-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2017-02-01 00:00:00
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journal_title：Journal of structural biology
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.2000.4315
更新日期：2000-11-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.2000.4320
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pub_type： 杂志文章
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更新日期：2014-06-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2016-06-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2008.02.009
更新日期：2008-06-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/s1047-8477(02)00035-7
更新日期：2002-06-01 00:00:00
abstract：:The small icosahedral plant RNA nepovirus Grapevine fanleaf virus (GFLV) is specifically transmitted by a nematode and causes major damage to vineyards worldwide. To elucidate the molecular mechanisms underlying the recognition between the surface of its protein capsid and cellular components of its vector, host and v...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2011.02.007
更新日期：2011-05-01 00:00:00
abstract：:The structure of Panicum Mosaic Virus (PMV) was determined by X-ray diffraction analysis to 2.9Å resolution. The crystals were of pseudo symmetry F23; the true crystallographic unit cell was of space group P2(1) with a=411.7Å, b=403.9Å and c=412.5Å, with β=89.7°. The asymmetric unit was two entire T=3 virus particles,...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2012.10.012
更新日期：2013-01-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章,评审
doi：10.1006/jsbi.1996.0007
更新日期：1996-01-01 00:00:00
abstract：:Tobacco streak virus (TSV), the type member of Ilarvirus genus, is a major plant pathogen. TSV purified from infected plants consists of a ss-RNA genome encapsidated in spheroidal particles with diameters of 27, 30 and 33nm constructed from multiple copies of a single species of coat protein (CP) subunits. Apart from ...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2015.12.007
更新日期：2016-02-01 00:00:00
abstract：:Alpha-helical bundles and beta-barrel proteins represent the two basic types of architecture known for integral membrane proteins. Irregular structural motifs have been revealed with the growing number of structures determined. "Discontinuous" helices are present in membrane proteins that actively transport ions. In t...
journal_title：Journal of structural biology
pub_type： 杂志文章,评审
doi：10.1016/j.jsb.2007.01.011
更新日期：2007-08-01 00:00:00
abstract：:A structural analysis of cells that contained the interferon-alpha-induced lupus inclusions (LI) was performed using a high-voltage electron microscope to determine the exact cellular location of LI and their association with normal cell organelles. LI were induced in the human B lymphoblastoid cell line, Daudi, by cu...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/1047-8477(92)90004-t
更新日期：1992-01-01 00:00:00
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更新日期：2004-06-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1016/1047-8477(90)90064-j
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更新日期：2012-02-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2006-04-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.1998.3970
更新日期：1998-01-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2013.02.010
更新日期：2013-10-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.2001.4343
更新日期：2001-01-01 00:00:00
abstract：:Membrane proteins perform many essential cellular functions. Over the last years, substantial advances have been made in our understanding of the structure and function of isolated membrane proteins. However, like soluble proteins, many membrane proteins assemble into supramolecular complexes that perform specific fun...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2007.07.009
更新日期：2007-12-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2009.10.005
更新日期：2010-02-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2008.12.001
更新日期：2009-04-01 00:00:00
abstract：:Single particle electron cryomicroscopy (cryo-EM) allows for structures of proteins and protein complexes to be determined from images of non-crystalline specimens. Cryo-EM data analysis requires electron microscope images of randomly oriented ice-embedded protein particles to be rotated and translated to allow for co...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2015.06.014
更新日期：2015-11-01 00:00:00
