Mechanisms in microbial evolution.

abstract：:Human alpha 2-macroglobulin (alpha 2M), a large tetrameric plasma glycoprotein, inhibits a wide spectrum of proteinases by a particular "trapping" mechanism resulting from the proteolysis of peptide bonds at specific "bait" regions. This induces the hydrolysis of four thiol esters triggering both the possible covalent...

journal_title：Journal of structural biology

authors： Boisset N,Taveau JC,Pochon F,Barray M,Delain E,Lamy JN

更新日期：1991-02-01 00:00:00

abstract：:With the development of Cryo Electron Microscopy Of Vitreous Sections (CEMOVIS), imaging cells in a close to native state has become a reality. However with the commonly used carriers for high-pressure freezing and cryo-sectioning, adherent grown cells either need to be detached from their substrate. Here a new method...

journal_title：Journal of structural biology

authors： Mesman RJ

更新日期：2013-09-01 00:00:00

abstract：:Human scalp hairs are often examined microscopically to study the variation and diversity among a range of visible morphological traits. In this study, we focused on the ultrastructure of human scalp hair within its keratinized matrix, emphasizing, the density and distribution of melanosomes, variation in cuticle thic...

journal_title：Journal of structural biology

authors： Koch SL,Shriver MD,Jablonski NG

更新日期：2019-01-01 00:00:00

abstract：:HIV-1 Rev mediates the nuclear export of unspliced and partially-spliced viral transcripts for the production of progeny genomes and structural proteins. In this process, four (or more) copies of Rev assemble onto a highly-structured 351-nt region in such viral transcripts, the Rev response element (RRE). How this occ...

journal_title：Journal of structural biology

authors： Watts NR,Eren E,Zhuang X,Wang YX,Steven AC,Wingfield PT

更新日期：2018-08-01 00:00:00

abstract：:COVID-19 pandemic, caused by SARS-CoV-2, has drastically affected human health all over the world. After the emergence of the pandemic the major focus of efforts to attenuate the infection has been on repurposing the already approved drugs to treat COVID-19 adopting a fast-track strategy. However, to date a specific r...

journal_title：Journal of structural biology

authors： Shahid M,Shahzad-Ul-Hussan S

更新日期：2020-12-29 00:00:00

abstract：:The structure determination of macromolecular complexes is entering a new era. The methods of optical microscopy, electron microscopy, X-ray crystallography, and nuclear magnetic resonance increasingly are being combined in hybrid method approaches to achieve an integrated view of macromolecular complexes that span fr...

journal_title：Journal of structural biology

authors： Finzi D,Dieffenbach CW,Basavappa R

更新日期：2007-05-01 00:00:00

abstract：:Net-negatively-charged heterospecific A:B:C collagen peptide heterotrimers were designed using an automated computational approach. The design algorithm considers both target stability and the energy gap between the target states and misfolded competing states. Structural characterization indicates the net-negative ch...

journal_title：Journal of structural biology

authors： Parmar AS,Zahid S,Belure SV,Young R,Hasan N,Nanda V

更新日期：2014-02-01 00:00:00

abstract：:The structure of full-length human TLR5 determined by electron microscopy single-particle image reconstruction at 26Å resolution shows that TLR5 forms an asymmetric homodimer via ectodomain interactions. The structure shows that like TLR9, TLR5 dimerizes in the absence of ligand. The asymmetry of the dimer suggests th...

journal_title：Journal of structural biology

authors： Zhou K,Kanai R,Lee P,Wang HW,Modis Y

更新日期：2012-02-01 00:00:00

abstract：:Microcrystals of the chlorophyll binding protein, CP43, isolated from spinach thylakoid membranes have been studied by electron microscopy both in negative stain and in vitreous ice. Image analyses of three characteristic views show that the crystals are built of five different layers perpendicular to the c-axis. Each...

journal_title：Journal of structural biology

authors： Büchel C,Morris E,Barber J

更新日期：2000-09-01 00:00:00

abstract：:The structure of R-phycoerythrin (R-PE) from the red alga Griffithsia monilis was solved at 1.90-A resolution by molecular replacement, using the atomic coordinates of cyanobacterial phycocyanin from Fremyella diplosiphon as a model. The crystallographic R factor for the final model is 17.5% (Rfree 22.7%) for reflecti...

journal_title：Journal of structural biology

authors： Ritter S,Hiller RG,Wrench PM,Welte W,Diederichs K

更新日期：1999-06-15 00:00:00

abstract：:Two membrane-bound ATP-dependent AAA proteases conduct protein quality surveillance in the inner membrane of mitochondria and control crucial steps during mitochondrial biogenesis. AAA domains of proteolytic subunits are critical for the recognition of non-native membrane proteins which are extracted from the membrane...

journal_title：Journal of structural biology

authors： Graef M,Langer T

更新日期：2006-10-01 00:00:00

abstract：:Three questions central to understanding the initiation of DNA replication in eukaryotes are: (1) Does DNA synthesis begin at a defined place? (2) What determines replication initiation sites? (3) What regulates an origin to fire only once per cell cycle? A key player in this is the origin recognition complex (ORC), r...

journal_title：Journal of structural biology

authors： Gerbi SA,Strezoska Z,Waggener JM

更新日期：2002-10-01 00:00:00

abstract：:We have shown by in vitro development of staged follicles in the presence of tritiated proline, as well as by immunoblot analysis, that the Dvs19 and DVS15 chorion proteins of Drosophila virilis are synthesized by the follicular epithelium and are incorporated in the chorion at the terminal stages of choriogenesis. Li...

journal_title：Journal of structural biology

authors： Trougakos IP,Margaritis LH

更新日期：1998-10-01 00:00:00

abstract：:In many organisms, the ubiquitous second messenger cAMP is formed by at least one member of the adenylyl cyclase (AC) Class III. These ACs feature a conserved dimeric catalytic core architecture, either through homodimerization or through pseudo-heterodimerization of a tandem of two homologous catalytic domains, C1 an...

journal_title：Journal of structural biology

authors： Linder J,Hupfeld E,Weyand M,Steegborn C,Moniot S

更新日期：2020-08-01 00:00:00

abstract：:Cryoelectron microscopy makes it possible to record high-resolution detail from large and complex structures. However, its application to understanding cellular structure is limited by the requirement that samples should be no thicker than approximately 0.5-1 microm. Therefore it is important to develop the ability to...

journal_title：Journal of structural biology

authors： Luther PK,Morris EP

更新日期：2003-05-01 00:00:00

abstract：:Structures of biological macromolecules determined by transmission cryoelectron microscopy (cryo-TEM) and three-dimensional image reconstruction are often displayed as surface-shaded representations with depth cueing along the viewed direction (Z cueing). Depth cueing to indicate distance from the center of virus part...

journal_title：Journal of structural biology

authors： Spencer SM,Sgro JY,Dryden KA,Baker TS,Nibert ML

更新日期：1997-10-01 00:00:00

abstract：:The central shaft of a bird's flight feather bears most of the aerodynamic load during flight and exhibits some remarkable mechanical properties. The shaft comprises two parts, the calamus and the rachis. The calamus is at the base of the shaft, while the rachis is the longer upper part which supports the vanes. The s...

journal_title：Journal of structural biology

authors： Laurent CM,Dyke JM,Cook RB,Dyke G,de Kat R

更新日期：2020-07-01 00:00:00

abstract：:Brain deposition of Aβ in the form of amyloid plaques is a pathological hallmark of Alzheimer's disease. There are two major species of Aβ in the brain: Aβ42 and Aβ40. Although Aβ40 is several-fold more abundant than Aβ42 in soluble form, Aβ42 is the major component of amyloid plaques. Structural knowledge of Aβ42 fib...

journal_title：Journal of structural biology

authors： Gu L,Tran J,Jiang L,Guo Z

更新日期：2016-04-01 00:00:00

abstract：:It has been known since the 19th century that coral skeletons are built of aragonite crystals with taxonomy-linked arrangements, but the way by which each coral species controls this crystallization process remains an unsolved question. The problem became still more intriguing when it was shown that isotopic compositi...

journal_title：Journal of structural biology

authors： Cuif JP,Dauphin Y

更新日期：2005-06-01 00:00:00

abstract：:Fructokinase (FRK; EC 2.7.1.4) catalyzes the phosphorylation of d-fructose to d-fructose 6-phosphate (F6P). This irreversible and near rate-limiting step is a central and regulatory process in plants and bacteria, which channels fructose into a metabolically active state for glycolysis. Towards understanding the mecha...

journal_title：Journal of structural biology

authors： Chua TK,Seetharaman J,Kasprzak JM,Ng C,Patel BK,Love C,Bujnicki JM,Sivaraman J

更新日期：2010-09-01 00:00:00

abstract：:Tobacco mosaic virus (TMV) is a plant virus with a highly ordered organisation and has been described in three different structural states: As stacked disks without RNA (X-ray crystallography), as a helical form with RNA (X-ray fibre diffraction) and as a second distinct helical form with RNA (cryo-EM). Here we presen...

journal_title：Journal of structural biology

authors： Clare DK,Orlova EV

更新日期：2010-09-01 00:00:00

abstract：:Helicases are proteins that use the energy of ATP hydrolysis to open double-stranded DNA, RNA, or RNA-DNA hybrids into two single strands. Based upon sequence analysis, at least 12 helicases exist in Escherichia coli. We know that these proteins play important roles in DNA replication, recombination, repair, and trans...

journal_title：Journal of structural biology

authors： Egelman EH

更新日期：1998-12-15 00:00:00

abstract：:Formation of filaments by the Ure2 protein constitutes the molecular mechanism of the [URE3] prion in yeast. According to the "amyloid backbone" model, the N-terminal asparagine-rich domains of Ure2p polymerize to form an amyloid core fibril that is surrounded by C-terminal domains in their native conformation. Protea...

journal_title：Journal of structural biology

authors： Baxa U,Cheng N,Winkler DC,Chiu TK,Davies DR,Sharma D,Inouye H,Kirschner DA,Wickner RB,Steven AC

更新日期：2005-05-01 00:00:00

abstract：:In-cell NMR spectroscopy has gained recent popularity since it provides means to analyze the conformational and functional properties of proteins inside living cells and at atomic resolution. High-resolution in-cell NMR spectroscopy was originally established in bacterial cells and based on a rationale that relies on ...

journal_title：Journal of structural biology

authors： Selenko P,Wagner G

更新日期：2007-05-01 00:00:00

abstract：:The QVIEW image-processing software package provides a graphical user interface for interactive and rapid selection of particles from digital electron micrographs. This package was developed to facilitate the selection of elliptical, hydrophobic, and aggregating particles, which present complications for other particl...

journal_title：Journal of structural biology

authors： Shah AK,Stewart PL

更新日期：1998-09-01 00:00:00

abstract：:Members of the Ku superfamily are DNA-end-binding proteins involved in non-homologous end-joining (NHEJ) DNA repair. The published crystal structure of human Ku-DNA complex reveals a heterodimer that forms a ring around dsDNA by means of the Ku core modules. These modules contain a highly conserved seven-stranded β-ba...

journal_title：Journal of structural biology

authors： Krishna SS,Aravind L

更新日期：2010-12-01 00:00:00

abstract：:The cyclooxygenases (COX-1 and COX-2) catalyze the rate-limiting step in the biosynthesis of prostaglandins, and are the pharmacological targets of non-steroidal anti-inflammatory drugs (NSAIDs) and COX-2 selective inhibitors (coxibs). Ibuprofen (IBP) is one of the most commonly available over-the-counter pharmaceutic...

journal_title：Journal of structural biology

authors： Orlando BJ,Lucido MJ,Malkowski MG

更新日期：2015-01-01 00:00:00

abstract：:Electron cryo-tomography (cryoET) is currently the only technique that allows the direct observation of proteins in their native cellular environment. Sub-volume averaging of electron tomograms offers a route to increase the signal-to-noise of repetitive biological structures, such improving the information content an...

journal_title：Journal of structural biology

authors： Grange M,Vasishtan D,Grünewald K

更新日期：2017-02-01 00:00:00

abstract：:Regulation of gene transcription in both prokaryotes and eukaryotes involves the formation of DNA-multiprotein complexes. These complexes build a precise three-dimensional topology allowing communication between distal regions of DNA. The switch from early to late transcription in bacteriophage Ø29 involves binding of...

journal_title：Journal of structural biology

authors： del Arroyo PG,Vélez M,Piétrement O,Salas M,Carrascosa JL,Camacho A

更新日期：2009-12-01 00:00:00

abstract：:Tricorn protease from the archaeon Thermoplasma acidophilum acts "downstream" of the proteasome; in conjunction with its aminopeptidase cofactors it converts peptides generated by the proteasome into free amino acids. The basic functional unit of Tricorn is a homohexamer of the 121-kDa subunit, 20 of which can assembl...

journal_title：Journal of structural biology

authors： Walz J,Koster AJ,Tamura T,Baumeister W

更新日期：1999-12-01 00:00:00