Abstract：
:Three questions central to understanding the initiation of DNA replication in eukaryotes are: (1) Does DNA synthesis begin at a defined place? (2) What determines replication initiation sites? (3) What regulates an origin to fire only once per cell cycle? A key player in this is the origin recognition complex (ORC), required for assembly of the pre-replication complex (pre-RC), that is converted later to the initiation complex (IC). In both yeast ARS1 and DNA puff II/9A of the metazoan fly Sciara, there is a defined start site of replication adjacent to an ORC-binding site. Although ORC has some inherent preference for certain DNA sequences, other factors may also modulate its binding to DNA. The preferred site where DNA synthesis starts at Sciara II/9A and the boundaries of the initiation zone change during development, when DNA puff amplification occurs. The position of the initiation zone may be influenced by the transcriptional machinery and/or chromatin structure. With regard to the third question, rereplication of the whole genome in yeast occurs when components of the pre-RC are stabilized by mutation. In contrast, a locus-specific amplification factor probably exists to account for site-specific DNA amplification in flies.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Gerbi SA,Strezoska Z,Waggener JMdoi
10.1016/s1047-8477(02)00538-5keywords:
subject
Has Abstractpub_date
2002-10-01 00:00:00pages
17-30issue
1-3eissn
1047-8477issn
1095-8657pii
S1047847702005385journal_volume
140pub_type
杂志文章,评审abstract：:Rlf is a guanine nucleotide exchange factor for the small G-proteins RalA and RalB and couples Ras- to Ral-signalling. Here the crystal structure of the catalytic module of Rlf consisting of a REM- and a CDC25-homology domain is determined. The structure is distinguished by an extended three stranded β-sheet called th...
journal_title：Journal of structural biology
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2015-05-01 00:00:00
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更新日期：2001-09-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2010.04.003
更新日期：2010-07-01 00:00:00
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journal_title：Journal of structural biology
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doi：10.1016/j.jsb.2008.09.002
更新日期：2008-12-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2011-12-01 00:00:00
abstract：:A characteristic feature of the dense phases formed by fiber-shaped molecules is their organization into parallel rods packed in a hexagonal or pseudo-hexagonal lateral network. This is typically the case for the collagen triple helices inside fibrils, as confirmed by recent X-ray diffraction experiments carried out o...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2010.11.018
更新日期：2011-02-01 00:00:00
abstract：:In recent years, cryo-electron microscopy (cryo-EM) has established itself as a key method in structural biology, permitting the structural characterization of large biomolecular complexes in various functional states. The data obtained through single-particle cryo-EM has recently seen a leap in resolution thanks to l...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2010.09.024
更新日期：2011-03-01 00:00:00
abstract：:Cryo Electron Microscopy is a powerful Structural Biology technique, allowing the elucidation of the three-dimensional structure of biological macromolecules. In particular, the structural study of purified macromolecules -often referred as Single Particle Analysis(SPA)- is normally performed through an iterative proc...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2015.01.009
更新日期：2015-03-01 00:00:00
abstract：:The Caliciviridae is a family of nonenveloped, icosahedral, positive-sense single-stranded RNA viruses. This family of viruses consists of both animal and human pathogens. Adapting human caliciviruses to cell culture has not been successful, whereas some animal caliciviruses, including San Miguel sea lion virus, have ...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/s1047-8477(02)00583-x
更新日期：2003-02-01 00:00:00
abstract：:Chaperonins mediate protein folding in an ATP-dependent manner. ATP binding and hydrolysis by chaperonins are subject to both homotropic and heterotropic allosteric regulation. In the case of GroEL and CCT, homotropic regulation by ATP is manifested in nested cooperativity, which involves positive intra-ring cooperati...
journal_title：Journal of structural biology
pub_type： 杂志文章,评审
doi：10.1006/jsbi.2001.4377
更新日期：2001-08-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.2000.4229
更新日期：2000-04-01 00:00:00
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journal_title：Journal of structural biology
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doi：10.1016/j.jsb.2011.07.009
更新日期：2011-11-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2002-08-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2020-07-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2004.11.011
更新日期：2005-03-01 00:00:00
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journal_title：Journal of structural biology
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journal_title：Journal of structural biology
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更新日期：2010-10-01 00:00:00
abstract：:Plasminogen activator inhibitor-1 (PAI-1) is a serine protease inhibitor (serpin) that plays an important role in cardiovascular disorders and tumor development. The potential role of PAI-1 as a drug target has been evaluated in various animal models (e.g. mouse and rat). Sensitivity to PAI-1 inhibitory agents varied ...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2010.03.006
更新日期：2010-07-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2001-12-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2014-06-01 00:00:00
abstract：:Amelogenin is believed to be involved in controlling the formation of the highly anisotropic and ordered hydroxyapatite crystallites that form enamel. The adsorption behavior of amelogenin proteins onto substrates is very important because protein-surface interactions are critical to its function. We have previously u...
journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2010-03-01 00:00:00
