Abstract:
:Hard X-ray fluorescence microscopy and magnified phase contrast imaging are combined to obtain quantitative maps of the projected metal concentration in whole cells. The experiments were performed on freeze dried cells at the nano-imaging station ID22NI of the European Synchrotron Radiation Facility (ESRF). X-ray fluorescence analysis gives the areal mass of most major, minor and trace elements; it is validated using a biological standard of known composition. Quantitative phase contrast imaging provides maps of the projected mass and is validated using calibration samples and through comparison with Atomic Force Microscopy and Scanning Transmission Ion Microscopy. Up to now, absolute quantification at the sub-cellular level was impossible using X-ray fluorescence microscopy but can be reached with the use of the proposed approach.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Kosior E,Bohic S,Suhonen H,Ortega R,Devès G,Carmona A,Marchi F,Guillet JF,Cloetens Pdoi
10.1016/j.jsb.2011.12.005subject
Has Abstractpub_date
2012-02-01 00:00:00pages
239-47issue
2eissn
1047-8477issn
1095-8657pii
S1047-8477(11)00351-0journal_volume
177pub_type
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