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Chitin-based scaffolds are an integral part of the skeleton of the marine demosponge Ianthella basta.

Abstract：

:The skeletons of demosponges, such as Ianthella basta, are known to be a composite material containing organic constituents. Here, we show that a filigree chitin-based scaffold is an integral component of the I. basta skeleton. These chitin-based scaffolds can be isolated from the sponge skeletons using an isolation and purification technique based on treatment with alkaline solutions. Solid-state (13)C NMR, Raman, and FT-IR spectroscopies, as well as chitinase digestion, reveal that the isolated material indeed consists of chitin. The morphology of the scaffolds has been determined by light and electron microscopy. It consists of cross-linked chitin fibers approximately 40-100 nm in diameter forming a micro-structured network. The overall shape of this network closely resembles the shape of the integer sponge skeleton. Solid-state (13)C NMR spectroscopy was used to characterize the sponge skeleton on a molecular level. The (13)C NMR signals of the chitin-based scaffolds are relatively broad, indicating a high amount of disordered chitin, possibly in the form of surface-exposed molecules. X-ray diffraction confirms that the scaffolds isolated from I. basta consist of partially disordered and loosely packed chitin with large surfaces. The spectroscopic signature of these chitin-based scaffolds is closer to that of alpha-chitin than beta-chitin.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Brunner E,Ehrlich H,Schupp P,Hedrich R,Hunoldt S,Kammer M,Machill S,Paasch S,Bazhenov VV,Kurek DV,Arnold T,Brockmann S,Ruhnow M,Born R

doi

10.1016/j.jsb.2009.06.018

subject

Has Abstract

pub_date

2009-12-01 00:00:00

pages

539-47

issue

3

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(09)00169-5

journal_volume

168

pub_type

杂志文章

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