当前位置： SCI文献检索 > JOURNAL OF STRUCTURAL BIOLOGY期刊下所有文献 > Applications of a slow-scan CCD camera in protein electron crystallography.

Applications of a slow-scan CCD camera in protein electron crystallography.

Abstract：

:A Gatan 1024 x 1024 slow-scan charge-coupled device (CCD) camera has been interfaced to a JEOL4000EX electron cryomicroscope and explored for its usefulness in the electron crystallographic analysis of thin, glucose-embedded crystals of crotoxin complex kept at -125 degrees C. We show that the camera allows for an on-line assessment of the crystals' crystallinity, flatness, and thickness. Intensities obtained from electron diffraction patterns acquired with the camera have been statistically analyzed and were found to be consistent with theoretically expected values. A quantitative analysis of the diffraction intensity as function of the accumulated electron dose suggests the possibility of recording up to 250 diffraction patterns with 3.5 A resolution from a single crotoxin complex crystal 128 A thick. Tilt series of 125 electron diffraction patterns with 3.5 A data acquired from a single crystal are shown to be practically feasible. The current study demonstrates for the first time the effectiveness of using a slow-scan CCD camera for electron diffraction data collection from thin protein crystals at near atomic resolution.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Brink J,Chiu W

doi

10.1006/jsbi.1994.1029

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

23-34

issue

1

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(84)71029-X

journal_volume

113

pub_type

杂志文章

相关文献

JOURNAL OF STRUCTURAL BIOLOGY文献大全
  • Structural basis for pH dependent monomer-dimer transition of 3,4-dihydroxy 2-butanone-4-phosphate synthase domain from Mycobacterium tuberculosis.

    abstract：:3,4-dihydroxy 2-butanone 4-phosphate synthase (DHBPS) and GTP cyclohydrolase-II (GTPCH-II) are the two initial enzymes involved in riboflavin biosynthesis pathway, which has been shown to be essential for the pathogens. In Mycobacterium tuberculosis (Mtb), the ribA2 gene (Rv1415) encodes for the bi-functional enzyme w...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2011.01.013

    authors： Singh M,Kumar P,Karthikeyan S

    更新日期：2011-05-01 00:00:00

  • Structural and dynamics studies of the TetR family protein, CprB from Streptomyces coelicolor in complex with its biological operator sequence.

    abstract：:In Streptomycetes, tetracycline repressor family of transcription regulators (TetR-FTRs) controls various biological processes including antibiotic biosynthesis, cellular morphology and innate resistance. Here, we focus on understanding the structural basis of transcription regulation by CprB, a member of TetR-FTRs fr...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2017.03.006

    authors： Bhukya H,Jana AK,Sengupta N,Anand R

    更新日期：2017-05-01 00:00:00

  • Electron diffraction and high-resolution imaging on highly-crystalline β-chitin microfibril.

    abstract：:The ultrastructure of β-chitin microfibrils from a centric diatom, Thalassiosira, and a tubeworm, Lamellibrachia, was studied using electron diffraction and high-resolution electron microscopy. Electron microdiffraction diagrams corresponding to each projection of the β-chitin crystals were obtained, and all the data ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2011.07.001

    authors： Ogawa Y,Kimura S,Wada M

    更新日期：2011-10-01 00:00:00

  • Structural insights into biosynthesis of resorcinolic lipids by a type III polyketide synthase in Neurospora crassa.

    abstract：:Microbial type III polyketide synthases (PKSs) have revealed remarkable mechanistic as well as functional versatility. Recently, a type III PKS homolog from Azotobacter has been implicated in the biosynthesis of resorcinolic lipids, thus adding a new functional significance to this class of proteins. Here, we report t...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2008.02.009

    authors： Goyal A,Saxena P,Rahman A,Singh PK,Kasbekar DP,Gokhale RS,Sankaranarayanan R

    更新日期：2008-06-01 00:00:00

  • 3-dimensional imaging of collagen using second harmonic generation.

    abstract：:Collagen is the most important structural protein of the animal body. Its unique triple-helix structure and extremely high level of crystallinity make it exceptionally efficient in generating the second harmonic of incident light, and we show here how this leads to a novel mode of microscopy of immediate practical sig...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/s1047-8477(02)00576-2

    authors： Cox G,Kable E,Jones A,Fraser I,Manconi F,Gorrell MD

    更新日期：2003-01-01 00:00:00

  • A strategy for electron tomographic data collection and crystallographic reconstruction of biological bundles.

    abstract：:Structures of highly ordered biological bundles have unique features which call for special experimental and computational methods in electron cryomicroscopy. They can be considered as three-dimensional quasi-crystals and reconstructed using a crystallographic approach. However, they are neither "infinitely" large wit...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1997.3916

    authors： Sherman MB,Jakana J,Sun S,Matsudaira P,Chiu W,Schmid MF

    更新日期：1997-12-01 00:00:00

  • Structural analysis of DNA-PKcs: modelling of the repeat units and insights into the detailed molecular architecture.

    abstract：:DNA-dependent protein kinase (DNA-PK) is part of the eukaryotic DNA double strand break repair pathway and as such is crucial for maintenance of genomic stability, as well as for V(D)J (variable-diversity-joining) recombination. The catalytic subunit of DNA-PK (DNA-PKcs) belongs to the phosphatidylinositol-3 (PI-3) ki...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2003.11.024

    authors： Brewerton SC,Doré AS,Drake AC,Leuther KK,Blundell TL

    更新日期：2004-03-01 00:00:00

  • A high-throughput strategy to screen 2D crystallization trials of membrane proteins.

    abstract：:Electron microscopy of two-dimensional (2D) crystals has demonstrated potential for structure determination of membrane proteins. Technical limitations in large-scale crystallization screens have, however, prevented a major breakthrough in the routine application of this technology. Dialysis is generally used for dete...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2007.09.003

    authors： Vink M,Derr K,Love J,Stokes DL,Ubarretxena-Belandia I

    更新日期：2007-12-01 00:00:00

  • A surrogate structural platform informed by ancestral reconstruction and resurrection of a putative carbohydrate binding module hybrid illuminates the neofunctionalization of a pectate lyase.

    abstract：:Yersinia enterocolitica is a pectinolytic zoonotic foodborne pathogen, the genome of which contains pectin-binding proteins and several different classes of pectinases, including polysaccharide lyases (PLs) and an exopolygalacturonase. These proteins operate within a coordinated pathway to completely saccharify homoga...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2019.06.003

    authors： Jones DR,McLean R,Hobbs JK,Abbott DW

    更新日期：2019-09-01 00:00:00

  • Conjugational junctions: morphology of specific contacts in conjugating Escherichia coli bacteria.

    abstract：:F-plasmid-mediated bacterial conjugation was studied with hfr (traDts) and tra I mutant Escherichia coli donor strains. This allowed us to observe a statistically significant number of conjugation-specific contacts by video and electron microscopy. Single mating events between E. coli were observed in real time by vid...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/1047-8477(91)90018-r

    authors： Dürrenberger MB,Villiger W,Bächi T

    更新日期：1991-10-01 00:00:00

  • Interactions of the α-subunits of heterotrimeric G-proteins with GPCRs, effectors and RGS proteins: a critical review and analysis of interacting surfaces, conformational shifts, structural diversity and electrostatic potentials.

    abstract：:G-protein coupled receptors (GPCRs) are one of the largest families of membrane receptors in eukaryotes. Heterotrimeric G-proteins, composed of α, β and γ subunits, are important molecular switches in the mediation of GPCR signaling. Receptor stimulation after the binding of a suitable ligand leads to G-protein hetero...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2013.03.004

    authors： Baltoumas FA,Theodoropoulou MC,Hamodrakas SJ

    更新日期：2013-06-01 00:00:00

  • Application of isotope exchange based mass spectrometry to understand the mechanism of inhibition of sickle hemoglobin polymerization upon oxygenation.

    abstract：:Sickle hemoglobin (HbS) polymerization initiates in the deoxy state with the binding of hydrophobic patch formed by the isopropyl group of βVal6 residue of a hemoglobin tetramer with the hydrophobic pocket of another tetramer, whose hydrophobic patch binds to the hydrophobic groove of a third molecule. Subsequent elon...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2017.04.009

    authors： Das R,Mitra A,Bhat V,Mandal AK

    更新日期：2017-07-01 00:00:00

  • Defining and solving the essential protein-protein interactions in HIV infection.

    abstract：:The structure determination of macromolecular complexes is entering a new era. The methods of optical microscopy, electron microscopy, X-ray crystallography, and nuclear magnetic resonance increasingly are being combined in hybrid method approaches to achieve an integrated view of macromolecular complexes that span fr...

    journal_title：Journal of structural biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jsb.2006.10.028

    authors： Finzi D,Dieffenbach CW,Basavappa R

    更新日期：2007-05-01 00:00:00

  • Two different correlations between nanoindentation modulus and mineral content in the bone-cartilage interface.

    abstract：:The biomechanical properties of the zone of calcified cartilage (ZCC) in articulating joints are of clinical relevance due to the role ZCC plays in load transfer from cartilage to bone. To determine the micron-level mechanical properties and their correlation to mineral concentration in the ZCC, we combined nanoindent...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2004.10.010

    authors： Gupta HS,Schratter S,Tesch W,Roschger P,Berzlanovich A,Schoeberl T,Klaushofer K,Fratzl P

    更新日期：2005-02-01 00:00:00

  • The leucine rich amelogenin protein (LRAP) adsorbs as monomers or dimers onto surfaces.

    abstract：:Amelogenin is believed to be involved in controlling the formation of the highly anisotropic and ordered hydroxyapatite crystallites that form enamel. The adsorption behavior of amelogenin proteins onto substrates is very important because protein-surface interactions are critical to its function. We have previously u...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.10.007

    authors： Tarasevich BJ,Lea S,Shaw WJ

    更新日期：2010-03-01 00:00:00

  • Properties of the nonhelical end domains of vimentin suggest a role in maintaining intermediate filament network structure.

    abstract：:To investigate the functional role of the nonhelical domains of the intermediate filament (IF) protein vimentin, we carried out transient transfection of constructs encoding fusion proteins of these domains with enhanced green fluorescent protein (EGFP). Expression of these fusion proteins did not have any effect on t...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.2000.4315

    authors： Lowrie DJ Jr,Stickney JT,Ip W

    更新日期：2000-11-01 00:00:00

  • WITHDRAWN: Intrinsic molecular characteristics of inhibiting STAT3 activation and dimerization through targeting the phosphotyrosine binding site within the SH2 domain-A molecular dynamics study.

    abstract：:This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2013.02.005

    authors： Fan Y,Kong R,Tweardy DJ,Wong ST

    更新日期：2013-02-18 00:00:00

  • Denoising of high-resolution single-particle electron-microscopy density maps by their approximation using three-dimensional Gaussian functions.

    abstract：:Cryo-electron microscopy (cryo-EM) of frozen-hydrated preparations of isolated macromolecular complexes is the method of choice to obtain the structure of complexes that cannot be easily studied by other experimental methods due to their flexibility or large size. An increasing number of macromolecular structures are ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2016.04.007

    authors： Jonić S,Vargas J,Melero R,Gómez-Blanco J,Carazo JM,Sorzano CO

    更新日期：2016-06-01 00:00:00

  • Three-dimensional network of Drosophila brain hemisphere.

    abstract：:The first step to understanding brain function is to determine the brain's network structure. We report a three-dimensional analysis of the brain network of the fruit fly Drosophila melanogaster by synchrotron-radiation tomographic microscopy. A skeletonized wire model of the left half of the brain network was built b...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2013.08.012

    authors： Mizutani R,Saiga R,Takeuchi A,Uesugi K,Suzuki Y

    更新日期：2013-11-01 00:00:00

  • Rapid near-atomic resolution single-particle 3D reconstruction with SIMPLE.

    abstract：:Cryogenic electron microscopy (cryo-EM) and single-particle analysis enables determination of near-atomic resolution structures of biological molecules. However, large computational requirements limit throughput and rapid testing of new image processing tools. We developed PRIME, an algorithm part of the SIMPLE softwa...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.08.005

    authors： Reboul CF,Kiesewetter S,Eager M,Belousoff M,Cui T,De Sterck H,Elmlund D,Elmlund H

    更新日期：2018-11-01 00:00:00

  • Photonic force microscope based on optical tweezers and two-photon excitation for biological applications.

    abstract：:A new scanning probe microscope, the photonic force microscope (PFM), based on optical tweezers and two-photon absorption processes for biological applications is described. Optical tweezers are used to trap a fluorescent latex bead with a diameter of 200 nm in an aqueous solution in all three dimensions. The fluoresc...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1997.3880

    authors： Florin EL,Pralle A,Hörber JK,Stelzer EH

    更新日期：1997-07-01 00:00:00

  • Molecular events during the early stages of aggregation of GNNQQNY: An all atom MD simulation study of randomly dispersed peptides.

    abstract：:This study probes the early events during lag phase of aggregation of GNNQQNY using all atom MD simulations in explicit solvent. Simulations were performed by varying system size, temperature and starting configuration. Peptides dispersed randomly in the simulation box come together early on in the simulation and form...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2015.09.020

    authors： Srivastava A,Balaji PV

    更新日期：2015-12-01 00:00:00

  • The structural characteristics of mononuclear-macrophage membrane observed by atomic force microscopy.

    abstract：:Mononuclear macrophages are important immune cells in the organisms. The complicated membrane structure underlying the diverse functions of mononuclear-macrophage has been largely unresolved. As a representative of monocyte-derived macrophages, the membrane structure of PMA differentiated THP-1 cells was comprehensive...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2019.04.002

    authors： Tian Y,Wu Y,Liu L,He L,Gao J,Zhou L,Yu F,Yu S,Wang H

    更新日期：2019-06-01 00:00:00

  • 3D microstructural study of selachimorph enameloid evolution.

    abstract：:Enameloid, the hyper-mineralized tissue covering shark teeth is a complex structure resulting from both ameloblast and odontoblast activity. The way these two types of cells interact to set up this tissue is not fully understood and results in the formation of subunits in the enameloid: the Single Crystallite Enameloi...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2020.107664

    authors： Fellah C,Douillard T,Maire E,Meille S,Reynard B,Cuny G

    更新日期：2020-11-19 00:00:00

  • Solution structure of the cyclic-nucleotide binding homology domain of a KCNH channel.

    abstract：:The carboxy-terminal region of the KCNH family of potassium channels contains a cyclic-nucleotide binding homology domain (CNBHD) that is important for channel gating and trafficking. The solution structure of the CNBHD of the KCNH potassium of zebrafish was determined using solution NMR spectroscopy. This domain exis...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2014.03.008

    authors： Li Q,Ng HQ,Yoon HS,Kang C

    更新日期：2014-04-01 00:00:00

  • Utility of Butvar support film and methylamine tungstate stain in three-dimensional electron microscopy: agreement between stain and frozen-hydrated reconstructions.

    abstract：:A random conical tilt reconstruction of negatively stained Saccharomyces cerevisiae fatty acid synthase was used as a model to compute a three-dimensional reconstruction from untilted stain specimens of the molecules in multiple orientations using a three-dimensional projection alignment method. The resulting structur...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1997.3911

    authors： Kolodziej SJ,Penczek PA,Stoops JK

    更新日期：1997-11-01 00:00:00

  • Insights into the composition, morphology, and formation of the calcareous shell of the serpulid Hydroides dianthus.

    abstract：:To date, the calcareous tubes of serpulid marine worms have not been studied extensively in a biomineralization context. The structure and composition of the tube shell and adhesive cement of the marine tubeworm Hydroides dianthus were studied using a variety of characterization techniques, including powder XRD, FTIR,...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.09.008

    authors： Tanur AE,Gunari N,Sullan RM,Kavanagh CJ,Walker GC

    更新日期：2010-02-01 00:00:00

  • Large nuclear RNP particles--the nuclear pre-mRNA processing machine.

    abstract：:Processing of nuclear pre-mRNA is an important step in the regulation of gene expression and involves 5(')- and 3(')-end processing, splicing, and editing. Mammalian nuclear pre-mRNAs are assembled in large ribonucleoprotein (lnRNP) complexes, in which the entire population of nuclear pre-mRNA is individually packaged...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/s1047-8477(02)00541-5

    authors： Raitskin O,Angenitzki M,Sperling J,Sperling R

    更新日期：2002-10-01 00:00:00

  • Conformational plasticity of the response regulator CpxR, a key player in Gammaproteobacteria virulence and drug-resistance.

    abstract：:The transcriptional regulator CpxR mediates an adaptive response to envelope stress, tightly linked to virulence and antibiotics resistance in several Gammaproteobacteria pathogens. In this work, we integrated crystallographic and small-angle X-ray scattering data to gain insights into the structure and conformational...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.08.001

    authors： Mechaly AE,Haouz A,Sassoon N,Buschiazzo A,Betton JM,Alzari PM

    更新日期：2018-11-01 00:00:00

  • Membrane-induced structure of the mammalian tachykinin neuropeptide gamma.

    abstract：:Neuropeptide gamma (NPgamma) is a neurokinin-2 (NK-2) receptor selective agonist, which plays an important role in mediation of asthma and elicits a wide range of biological responses like bronchoconstriction, vasodepression and regulation of endocrine functions. The structure determination of this peptide agonist is ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2004.08.008

    authors： Chandrashekar IR,Dike A,Cowsik SM

    更新日期：2004-12-01 00:00:00