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Solution structure of the cyclic-nucleotide binding homology domain of a KCNH channel.

Abstract：

:The carboxy-terminal region of the KCNH family of potassium channels contains a cyclic-nucleotide binding homology domain (CNBHD) that is important for channel gating and trafficking. The solution structure of the CNBHD of the KCNH potassium of zebrafish was determined using solution NMR spectroscopy. This domain exists as a monomer under solution conditions and adopts a similar fold to that determined by X-ray crystallography. The CNBHD does not bind cAMP because residue Y740 blocks the entry of cyclic-nucleotide to the binding pocket. Relaxation results show that the CNBHD is rigid except that some residues in the loop between β6 and β7 are flexible. Our results will be useful to understand the gating mechanism of KCNH family members through the CNBHD.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Li Q,Ng HQ,Yoon HS,Kang C

doi

10.1016/j.jsb.2014.03.008

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

68-74

issue

1

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(14)00061-6

journal_volume

186

pub_type

杂志文章

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