Solution structure of the cyclic-nucleotide binding homology domain of a KCNH channel.


:The carboxy-terminal region of the KCNH family of potassium channels contains a cyclic-nucleotide binding homology domain (CNBHD) that is important for channel gating and trafficking. The solution structure of the CNBHD of the KCNH potassium of zebrafish was determined using solution NMR spectroscopy. This domain exists as a monomer under solution conditions and adopts a similar fold to that determined by X-ray crystallography. The CNBHD does not bind cAMP because residue Y740 blocks the entry of cyclic-nucleotide to the binding pocket. Relaxation results show that the CNBHD is rigid except that some residues in the loop between β6 and β7 are flexible. Our results will be useful to understand the gating mechanism of KCNH family members through the CNBHD.


J Struct Biol


Li Q,Ng HQ,Yoon HS,Kang C




Has Abstract


2014-04-01 00:00:00














  • The three-dimensional structure of trypsin-treated Staphylococcus aureus alpha-toxin.

    abstract::Trypsin treatment of staphylococcal alpha-toxin cleaves the molecule into two roughly equally sized parts, which results in inactivation of the toxin. Tetragonal arrays of oligomers, closely resembling the native ones, can however be formed on lipid layers. From tilted views of negatively stained crystals a 3D structu...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Olofsson A,Kavéus U,Thelestam M,Hebert H

    更新日期:1992-05-01 00:00:00

  • IRIS explorer software for radial-depth cueing reovirus particles and other macromolecular structures determined by cryoelectron microscopy and image reconstruction.

    abstract::Structures of biological macromolecules determined by transmission cryoelectron microscopy (cryo-TEM) and three-dimensional image reconstruction are often displayed as surface-shaded representations with depth cueing along the viewed direction (Z cueing). Depth cueing to indicate distance from the center of virus part...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Spencer SM,Sgro JY,Dryden KA,Baker TS,Nibert ML

    更新日期:1997-10-01 00:00:00

  • Three different approaches for calculating the three-dimensional structure of microtubules decorated with kinesin motor domains.

    abstract::We have used three different electron microscopy approaches to calculate three-dimensional maps of tubulin assemblies decorated with the motor domain of kinesin. The approaches used were: (1) Tilt series reconstruction of negatively stained tubulin sheets. (2) Back-projection reconstruction of microtubules in ice. (3)...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Sosa H,Hoenger A,Milligan RA

    更新日期:1997-03-01 00:00:00

  • Single-particle tracking of oriC-GFP fluorescent spots during chromosome segregation in Escherichia coli.

    abstract::DNA regions close to the origin of replication were visualized by the green fluorescent protein (GFP)-Lac repressor/lac operator system. The number of oriC-GFP fluorescent spots per cell and per nucleoid in batch-cultured cells corresponded to the theoretical DNA replication pattern. A similar pattern was observed in ...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Elmore S,Müller M,Vischer N,Odijk T,Woldringh CL

    更新日期:2005-09-01 00:00:00

  • Nuclear structure and gene activity in human differentiated cells.

    abstract::The nuclear arrangement of the ABL, c-MYC, and RB1 genes was quantitatively investigated in human undifferentiated HL-60 cells and in a terminally differentiated population of human granulocytes. The ABL gene was expressed in both cell types, the c-MYC gene was active in HL-60 cells and down-regulated in granulocytes,...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Bártová E,Kozubek S,Jirsová P,Kozubek M,Gajová H,Lukásová E,Skalníková M,Ganová A,Koutná I,Hausmann M

    更新日期:2002-08-01 00:00:00

  • Seeds to crystals.

    abstract::Seeding has been critical for obtaining diffraction-quality crystals for many structures. In this article, applications and recommendations for seeding are presented based on examples from our laboratory and other groups. The implementation of seeding in high-throughput crystallization, robotics, and other emerging te...

    journal_title:Journal of structural biology

    pub_type: 杂志文章,评审


    authors: Bergfors T

    更新日期:2003-04-01 00:00:00

  • Nuclear lamina at the crossroads of the cytoplasm and nucleus.

    abstract::The nuclear lamina is a protein meshwork that lines the nuclear envelope in metazoan cells. It is composed largely of a polymeric assembly of lamins, which comprise a distinct sequence homology class of the intermediate filament protein family. On the basis of its structural properties, the lamina originally was propo...

    journal_title:Journal of structural biology

    pub_type: 杂志文章,评审


    authors: Gerace L,Huber MD

    更新日期:2012-01-01 00:00:00

  • Applications of the molecular dynamics flexible fitting method.

    abstract::In recent years, cryo-electron microscopy (cryo-EM) has established itself as a key method in structural biology, permitting the structural characterization of large biomolecular complexes in various functional states. The data obtained through single-particle cryo-EM has recently seen a leap in resolution thanks to l...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Trabuco LG,Schreiner E,Gumbart J,Hsin J,Villa E,Schulten K

    更新日期:2011-03-01 00:00:00

  • Crystal structures of salicylate 1,2-dioxygenase-substrates adducts: A step towards the comprehension of the structural basis for substrate selection in class III ring cleaving dioxygenases.

    abstract::The crystallographic structures of the adducts of salicylate 1,2-dioxygenase (SDO) with substrates salicylate, gentisate and 1-hydroxy-2-naphthoate, obtained under anaerobic conditions, have been solved and analyzed. This ring fission dioxygenase from the naphthalenesulfonate-degrading bacterium Pseudaminobacter salic...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Ferraroni M,Matera I,Steimer L,Bürger S,Scozzafava A,Stolz A,Briganti F

    更新日期:2012-02-01 00:00:00

  • Structures of PspAG97A α-glucoside hydrolase reveal a novel mechanism for chloride induced activation.

    abstract::Here we report the first crystal structure of a secretory α-glucoside hydrolase isolated from Pseudoalteromonas sp. K8, PspAG97A, which belongs to glycoside hydrolase family 97 and exhibits halophilic property. PspAG97A lacks an acidic surface, that is considered essential for protein stability at high salinity. Inter...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: He C,Li J,Li W,Xue Y,Fang Z,Fang W,Zhang X,Wang X,Xiao Y

    更新日期:2016-12-01 00:00:00

  • Axially aligned organic fibers and amorphous calcium phosphate form the claws of a terrestrial isopod (Crustacea).

    abstract::Skeletal elements that are exposed to heavy mechanical loads may provide important insights into the evolutionary solutions to mechanical challenges. We analyzed the microscopic architecture of dactylus claws in the woodlice Porcellio scaber and correlated these observations with analyses of the claws' mineral composi...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Vittori M,Srot V,Žagar K,Bussmann B,van Aken PA,Čeh M,Štrus J

    更新日期:2016-08-01 00:00:00

  • Investigation of the human pineal gland 3D organization by X-ray phase contrast tomography.

    abstract::Pineal gland (PG) is a part of the human brain epithalamus that plays an important role in sleep, circadian rhythm, immunity, and reproduction. The calcium deposits and lesions in PG interfere with normal function of the organ and can be associated with different health disorders including serious neurological disease...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Bukreeva I,Junemann O,Cedola A,Palermo F,Maugeri L,Begani Provinciali G,Pieroni N,Sanna A,Otlyga DA,Buzmakov A,Krivonosov Y,Zolotov D,Chukalina M,Ivanova A,Saveliev S,Asadchikov V,Fratini M

    更新日期:2020-12-01 00:00:00

  • Structure of the Pseudomonas aeruginosa XcpT pseudopilin, a major component of the type II secretion system.

    abstract::The bacterial type II protein secretion (T2S) and type IV piliation (T4P) systems share several common features. In particular, it is well established that the T2S system requires the function of a pilus-like structure, called pseudopilus, which is built upon assembly of pilin-like subunits, called pseudopilins. Pilin...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Alphonse S,Durand E,Douzi B,Waegele B,Darbon H,Filloux A,Voulhoux R,Bernard C

    更新日期:2010-01-01 00:00:00

  • STEM tomography analysis of the trypanosome transition zone.

    abstract::The protist Trypanosoma brucei is an emerging model for the study of cilia and flagella. Here, we used scanning transmission electron microscopy (STEM) tomography to describe the structure of the trypanosome transition zone (TZ). At the base of the TZ, nine transition fibres irradiate from the B microtubule of each do...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Trépout S,Tassin AM,Marco S,Bastin P

    更新日期:2018-04-01 00:00:00

  • Video on the Internet: An introduction to the digital encoding, compression, and transmission of moving image data.

    abstract::In this paper, we seek to provide an introduction to the fast-moving field of digital video on the Internet, from the viewpoint of the biological microscopist who might wish to store or access videos, for instance in image databases such as the BioImage Database ( We describe and evaluate the ...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Boudier T,Shotton DM

    更新日期:1999-04-01 00:00:00

  • Identification of crystalline material found in the thallus of the lichen, Myelochroa leucotyliza.

    abstract::Lichens are symbiotic associations of fungi and algae (or cyanobacteria). Fungal cells produce large amounts of lipid, assisted by algae, and secrete them out of the cells. Some of the secreted lipids crystallize in the thallus of the lichen. The crystalline materials sometimes occupy 30% of total dry weight of the th...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Arakawa-Kobayashi S,Kobayashi T,Hasebe M,Kanaseki T

    更新日期:2004-06-01 00:00:00

  • Crystallographic studies of ligand binding by Zn-alpha2-glycoprotein.

    abstract::Zn-alpha2-glycoprotein (ZAG) is a 41 kDa soluble protein that is present in most bodily fluids. The previously reported 2.8 A crystal structure of ZAG isolated from human serum demonstrated the structural similarity between ZAG and class I major histocompatibility complex (MHC) molecules and revealed a non-peptidic li...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Delker SL,West AP Jr,McDermott L,Kennedy MW,Bjorkman PJ

    更新日期:2004-11-01 00:00:00

  • Single particle reconstruction and validation using Bsoft for the map challenge.

    abstract::The Bsoft package is aimed at processing electron micrographs for the determination of the three-dimensional structures of biological specimens. Recent advances in hardware allow us to solve structures to near atomic resolution using single particle analysis (SPA). The Map Challenge offered me an opportunity to test t...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Heymann JB

    更新日期:2018-10-01 00:00:00

  • Expression, two-dimensional crystallization, and three-dimensional reconstruction of the beta8 outer membrane protein Omp21 from Comamonas acidovorans.

    abstract::The Omp21 protein from the proteobacterium Comamonas (Delftia) acidovorans belongs to the recently described beta8 family of outer membrane proteins, characterized by eight antiparallel beta-strands which form a beta-barrel. This family includes virulence proteins, OmpA and OmpX from Escherichia coli, and other relate...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Baldermann C,Engelhardt H

    更新日期:2000-08-01 00:00:00

  • Review: allostery in chaperonins.

    abstract::Chaperonins mediate protein folding in an ATP-dependent manner. ATP binding and hydrolysis by chaperonins are subject to both homotropic and heterotropic allosteric regulation. In the case of GroEL and CCT, homotropic regulation by ATP is manifested in nested cooperativity, which involves positive intra-ring cooperati...

    journal_title:Journal of structural biology

    pub_type: 杂志文章,评审


    authors: Horovitz A,Fridmann Y,Kafri G,Yifrach O

    更新日期:2001-08-01 00:00:00

  • Structure and function of Hsp78, the mitochondrial ClpB homolog.

    abstract::The cellular role of Hsp100/Clp chaperones in maintaining protein stability is based on two functional aspects. Under normal growth conditions they represent components of cellular protein quality control machineries that selectively remove damaged or misfolded polypeptides in cooperation with specific proteases. Afte...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Leidhold C,von Janowsky B,Becker D,Bender T,Voos W

    更新日期:2006-10-01 00:00:00

  • Looking into live cells with in-cell NMR spectroscopy.

    abstract::In-cell NMR spectroscopy has gained recent popularity since it provides means to analyze the conformational and functional properties of proteins inside living cells and at atomic resolution. High-resolution in-cell NMR spectroscopy was originally established in bacterial cells and based on a rationale that relies on ...

    journal_title:Journal of structural biology

    pub_type: 杂志文章,评审


    authors: Selenko P,Wagner G

    更新日期:2007-05-01 00:00:00

  • Substrate specific consequences of central pore mutations in the i-AAA protease Yme1 on substrate engagement.

    abstract::Two membrane-bound ATP-dependent AAA proteases conduct protein quality surveillance in the inner membrane of mitochondria and control crucial steps during mitochondrial biogenesis. AAA domains of proteolytic subunits are critical for the recognition of non-native membrane proteins which are extracted from the membrane...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Graef M,Langer T

    更新日期:2006-10-01 00:00:00

  • Structure of dihydropyrimidinase from Sinorhizobium meliloti CECT4114: new features in an amidohydrolase family member.

    abstract::The recombinant dihydropyrimidinase from Sinorhizobium meliloti CECT4114 (SmelDhp) has been characterised and its crystal structure elucidated at 1.85A. The global architecture of the protein is reminiscent of that of the amidohydrolase superfamily, consisting of two domains; an (alpha/beta)(8) TIM-like barrel domain,...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Martínez-Rodríguez S,Martínez-Gómez AI,Clemente-Jiménez JM,Rodríguez-Vico F,García-Ruíz JM,Las Heras-Vázquez FJ,Gavira JA

    更新日期:2010-02-01 00:00:00

  • Applications of a slow-scan CCD camera in protein electron crystallography.

    abstract::A Gatan 1024 x 1024 slow-scan charge-coupled device (CCD) camera has been interfaced to a JEOL4000EX electron cryomicroscope and explored for its usefulness in the electron crystallographic analysis of thin, glucose-embedded crystals of crotoxin complex kept at -125 degrees C. We show that the camera allows for an on-...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Brink J,Chiu W

    更新日期:1994-07-01 00:00:00

  • Crystallisation of CP43, a chlorophyll binding protein of photosystem II: an electron microscopy analysis of molecular packing.

    abstract::Microcrystals of the chlorophyll binding protein, CP43, isolated from spinach thylakoid membranes have been studied by electron microscopy both in negative stain and in vitreous ice. Image analyses of three characteristic views show that the crystals are built of five different layers perpendicular to the c-axis. Each...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Büchel C,Morris E,Barber J

    更新日期:2000-09-01 00:00:00

  • The leucine rich amelogenin protein (LRAP) adsorbs as monomers or dimers onto surfaces.

    abstract::Amelogenin is believed to be involved in controlling the formation of the highly anisotropic and ordered hydroxyapatite crystallites that form enamel. The adsorption behavior of amelogenin proteins onto substrates is very important because protein-surface interactions are critical to its function. We have previously u...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Tarasevich BJ,Lea S,Shaw WJ

    更新日期:2010-03-01 00:00:00

  • CTFFIND4: Fast and accurate defocus estimation from electron micrographs.

    abstract::CTFFIND is a widely-used program for the estimation of objective lens defocus parameters from transmission electron micrographs. Defocus parameters are estimated by fitting a model of the microscope's contrast transfer function (CTF) to an image's amplitude spectrum. Here we describe modifications to the algorithm whi...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Rohou A,Grigorieff N

    更新日期:2015-11-01 00:00:00

  • WITHDRAWN: Intrinsic molecular characteristics of inhibiting STAT3 activation and dimerization through targeting the phosphotyrosine binding site within the SH2 domain-A molecular dynamics study.

    abstract::This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at ...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Fan Y,Kong R,Tweardy DJ,Wong ST

    更新日期:2013-02-18 00:00:00

  • Bacillus licheniformis Anti-TRAP can assemble into two types of dodecameric particles with the same symmetry but inverted orientation of trimers.

    abstract::Anti-TRAP (AT) protein regulates expression of tryptophan biosynthetic genes by binding to the trp RNA-binding attenuation protein (TRAP) and preventing its interaction with RNA. Bacillus subtilis AT forms trimers that can either interact with TRAP or can further assemble into dodecameric particles. To determine which...

    journal_title:Journal of structural biology

    pub_type: 杂志文章


    authors: Shevtsov MB,Chen Y,Isupov MN,Leech A,Gollnick P,Antson AA

    更新日期:2010-04-01 00:00:00