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Two different correlations between nanoindentation modulus and mineral content in the bone-cartilage interface.

Abstract：

:The biomechanical properties of the zone of calcified cartilage (ZCC) in articulating joints are of clinical relevance due to the role ZCC plays in load transfer from cartilage to bone. To determine the micron-level mechanical properties and their correlation to mineral concentration in the ZCC, we combined nanoindentation (for micrometer level stiffness E(r) and hardness H) and quantitative back-scattered electron imaging or qBEI (for micrometer level mean calcium concentration Ca(Mean)) to study the ZCC-subchondral bone junction in 3 embedded human patellae. Nanoindentation line scans were correlated to qBEI analysis in the ZCC. The correlation between local stiffness and local mineral content was different in calcified cartilage compared to bone. The stiffness and hardness of calcified cartilage was typically lower than subchondral bone for the same mineral content. ZCC showed a wider range of variation in calcium content (1-28 wt %) compared to subchondral bone (16-26 wt %). 2D material property maps of the ZCC were generated from the mechanical-mineral correlation, showing that bands of high and low stiffness were found between the bone and tidemark, and between the ZCC and the unmineralized cartilage.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Gupta HS,Schratter S,Tesch W,Roschger P,Berzlanovich A,Schoeberl T,Klaushofer K,Fratzl P

doi

10.1016/j.jsb.2004.10.010

keywords:

subject

Has Abstract

pub_date

2005-02-01 00:00:00

pages

138-48

issue

2

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(04)00214-X

journal_volume

149

pub_type

杂志文章

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