当前位置： SCI文献检索 > JOURNAL OF STRUCTURAL BIOLOGY期刊下所有文献 > The bacteriocin AS-48 requires dimer dissociation followed by hydrophobic interactions with the membrane for antibacterial activity.

The bacteriocin AS-48 requires dimer dissociation followed by hydrophobic interactions with the membrane for antibacterial activity.

Abstract：

:The molecular mechanism underlining the antibacterial activity of the bacteriocin AS-48 is not known, and two different and opposite alternatives have been proposed. Available data suggested that the interaction of positively charged amino acids of AS-48 with the membrane would produce membrane destabilization and disruption. Alternatively, it has been proposed that AS-48 activity could rely on the effective insertion of the bacteriocin into the membrane. The biological and structural properties of the AS-48G13K/L40K double mutant were investigated to shed light on this subject. Compared with the wild type, the mutant protein suffered an important reduction in the antibacterial activity. Biochemical and structural studies of AS-48G13K/L40K mutant suggest the basis of its decreased antimicrobial activity. Lipid cosedimentation assays showed that the membrane affinity of AS-48G13K/L40K is 12-fold lower than that observed for the wild type. L40K mutation is responsible for this reduced membrane affinity and thus, hydrophobic interactions are involved in membrane association. Furthermore, the high-resolution crystal structure of AS-48G13K/L40K, together with the study of its dimeric character in solution showed that G13K stabilizes the inactive water-soluble dimer, which displays a reduced dipole moment. Our data suggest that the cumulative effect of these three affected properties reduces AS-48 activity, and point out that the bactericidal effect is achieved by the electrostatically driven approach of the inactive water-soluble dimer towards the membrane, followed by the dissociation and insertion of the protein into the lipid bilayer.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Cebrián R,Martínez-Bueno M,Valdivia E,Albert A,Maqueda M,Sánchez-Barrena MJ

doi

10.1016/j.jsb.2015.03.006

subject

Has Abstract

pub_date

2015-05-01 00:00:00

pages

162-72

issue

2

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(15)00068-4

journal_volume

190

pub_type

杂志文章

相关文献

JOURNAL OF STRUCTURAL BIOLOGY文献大全
  • Symmetry: A guide to its application in 2D electron crystallography.

    abstract：:A defining property of a crystal is its symmetry. This mini-review sets out to summarize all aspects that define 2D crystallographic symmetry as applied to the study of macromolecular structure. It begins by defining molecular point symmetries, before covering crystallographic symmetry operations in 2D, common notatio...

    journal_title：Journal of structural biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jsb.2007.07.002

    authors： Landsberg MJ,Hankamer B

    更新日期：2007-12-01 00:00:00

  • Three-dimensional reconstruction of collagen-proteoglycan interactions in the mouse corneal stroma by electron tomography.

    abstract：:Corneal transparency is fundamental to the visual system, and is directly related to the ordered collagen fibril architecture that the cornea maintains. Proteoglycans, through their protein core and highly anionic glycosaminoglycan side chains, are thought to regulate the collagen organisation in the corneal stroma. T...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2010.01.019

    authors： Parfitt GJ,Pinali C,Young RD,Quantock AJ,Knupp C

    更新日期：2010-05-01 00:00:00

  • Substrate specific consequences of central pore mutations in the i-AAA protease Yme1 on substrate engagement.

    abstract：:Two membrane-bound ATP-dependent AAA proteases conduct protein quality surveillance in the inner membrane of mitochondria and control crucial steps during mitochondrial biogenesis. AAA domains of proteolytic subunits are critical for the recognition of non-native membrane proteins which are extracted from the membrane...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2006.01.009

    authors： Graef M,Langer T

    更新日期：2006-10-01 00:00:00

  • Know your detergents: A case study on detergent background in negative stain electron microscopy.

    abstract：:Electron cryo-microscopy (cryo-EM) of purified macromolecular complexes is now providing 3D-structures at near-atomic resolution (Kühlbrandt, 2014). Cryo-EM can tolerate heterogeneous specimens, however, high-resolution efforts demand highly optimized samples. Therefore, significant pre-screening and evaluation is ess...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.05.008

    authors： Gewering T,Januliene D,Ries AB,Moeller A

    更新日期：2018-09-01 00:00:00

  • The structure of ibuprofen bound to cyclooxygenase-2.

    abstract：:The cyclooxygenases (COX-1 and COX-2) catalyze the rate-limiting step in the biosynthesis of prostaglandins, and are the pharmacological targets of non-steroidal anti-inflammatory drugs (NSAIDs) and COX-2 selective inhibitors (coxibs). Ibuprofen (IBP) is one of the most commonly available over-the-counter pharmaceutic...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2014.11.005

    authors： Orlando BJ,Lucido MJ,Malkowski MG

    更新日期：2015-01-01 00:00:00

  • 3D microstructural study of selachimorph enameloid evolution.

    abstract：:Enameloid, the hyper-mineralized tissue covering shark teeth is a complex structure resulting from both ameloblast and odontoblast activity. The way these two types of cells interact to set up this tissue is not fully understood and results in the formation of subunits in the enameloid: the Single Crystallite Enameloi...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2020.107664

    authors： Fellah C,Douillard T,Maire E,Meille S,Reynard B,Cuny G

    更新日期：2020-11-19 00:00:00

  • Application of isotope exchange based mass spectrometry to understand the mechanism of inhibition of sickle hemoglobin polymerization upon oxygenation.

    abstract：:Sickle hemoglobin (HbS) polymerization initiates in the deoxy state with the binding of hydrophobic patch formed by the isopropyl group of βVal6 residue of a hemoglobin tetramer with the hydrophobic pocket of another tetramer, whose hydrophobic patch binds to the hydrophobic groove of a third molecule. Subsequent elon...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2017.04.009

    authors： Das R,Mitra A,Bhat V,Mandal AK

    更新日期：2017-07-01 00:00:00

  • A new HIV-1 Rev structure optimizes interaction with target RNA (RRE) for nuclear export.

    abstract：:HIV-1 Rev mediates the nuclear export of unspliced and partially-spliced viral transcripts for the production of progeny genomes and structural proteins. In this process, four (or more) copies of Rev assemble onto a highly-structured 351-nt region in such viral transcripts, the Rev response element (RRE). How this occ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.03.011

    authors： Watts NR,Eren E,Zhuang X,Wang YX,Steven AC,Wingfield PT

    更新日期：2018-08-01 00:00:00

  • Importance of detergent and phospholipid in the crystallization of the human erythrocyte anion-exchanger membrane domain.

    abstract：:Three-dimensional crystals were obtained for the membrane domain of the human erythrocyte anion exchanger (AE1, Band 3). Protein homogeneity and stability and the delicate balance between the detergent used and the amount of phospholipids copurifying are critical to the formation of three-dimensional crystals of the A...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/s1047-8477(02)00010-2

    authors： Lemieux MJ,Reithmeier RA,Wang DN

    更新日期：2002-03-01 00:00:00

  • Crystallization and characterization of PU.1/IRF-4/DNA ternary complex.

    abstract：:PU.1 and IRF-4, members of the Ets and interferon regulatory families of transcription factors, respectively, form a cooperative ternary complex that is implicated in the regulation of B-cell-specific gene expression. A portion of the cooperativity involves interaction between the PU.1 and IRF-4 DNA-binding domains. W...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/s1047-8477(02)00514-2

    authors： Escalante CR,Shen L,Escalante MC,Brass AL,Edwards TA,Singh H,Aggarwal AK

    更新日期：2002-07-01 00:00:00

  • A method for establishing the handedness of biological macromolecules.

    abstract：:When biological macromolecules are imaged in the transmission electron microscope (TEM), their inherent handedness is lost because the three-dimensional (3D) structure is projected onto a two-dimensional (2D) plane, and identical 2D projections can be made from either 3D enantiomer. Nevertheless, tilt experiments in t...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1997.3896

    authors： Belnap DM,Olson NH,Baker TS

    更新日期：1997-10-01 00:00:00

  • Analysis of the interaction between the eukaryotic chaperonin CCT and its substrates actin and tubulin.

    abstract：:Two mechanisms have thus far been characterized for the assistance by chaperonins of the folding of other proteins. The first and best described is that of the prokaryotic chaperonin GroEL, which interacts with a large spectrum of proteins. GroEL uses a nonspecific mechanism by which any conformation of practically an...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.2001.4359

    authors： Llorca O,Martín-Benito J,Gómez-Puertas P,Ritco-Vonsovici M,Willison KR,Carrascosa JL,Valpuesta JM

    更新日期：2001-08-01 00:00:00

  • Unique three-dimensional structure of a fish pharyngeal jaw subjected to unusually high mechanical loads.

    abstract：:We examine the structure of the bone of the pharyngeal jaws of a large fish, the black drum (Pogonias cromis), that uses its tooth-jaw complex to crush hard-shelled bivalve mollusks. During mastication huge compressive forces are concentrated in a tiny zone at the tooth-bone interface. We report on the structure of th...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2020.107530

    authors： Raguin E,Rechav K,Brumfeld V,Shahar R,Weiner S

    更新日期：2020-08-01 00:00:00

  • Structure of Fusarium poae virus 1 shows conserved and variable elements of partitivirus capsids and evolutionary relationships to picobirnavirus.

    abstract：:Filamentous fungus Fusarium poae is a worldwide cause of the economically important disease Fusarium head blight of cereal grains. The fungus is itself commonly infected with a bisegmented dsRNA virus from the family Partitiviridae. For this study, we determined the structure of partitivirus Fusarium poae virus 1 (FpV...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2010.06.022

    authors： Tang J,Ochoa WF,Li H,Havens WM,Nibert ML,Ghabrial SA,Baker TS

    更新日期：2010-12-01 00:00:00

  • Depth-resolved structural imaging by third-harmonic generation microscopy.

    abstract：:Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third ha...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/S1047-8477(03)00125-4

    authors： Oron D,Yelin D,Tal E,Raz S,Fachima R,Silberberg Y

    更新日期：2004-07-01 00:00:00

  • A Bayesian adaptive basis algorithm for single particle reconstruction.

    abstract：:Traditional single particle reconstruction methods use either the Fourier or the delta function basis to represent the particle density map. This paper proposes a more flexible algorithm that adaptively chooses the basis based on the data. Because the basis adapts to the data, the reconstruction resolution and signal-...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2012.04.012

    authors： Kucukelbir A,Sigworth FJ,Tagare HD

    更新日期：2012-07-01 00:00:00

  • Automatic alignment of transmission electron microscope tilt series without fiducial markers.

    abstract：:Accurate image alignment is needed for computing three-dimensional reconstructions from transmission electron microscope tilt series. So far, the best results have been obtained by using colloidal gold beads as fiducial markers. If their use has not been possible for some reason, the only option has been the automatic...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.2001.4443

    authors： Brandt S,Heikkonen J,Engelhardt P

    更新日期：2001-12-01 00:00:00

  • Description and comparison of algorithms for correcting anisotropic magnification in cryo-EM images.

    abstract：:Single particle electron cryomicroscopy (cryo-EM) allows for structures of proteins and protein complexes to be determined from images of non-crystalline specimens. Cryo-EM data analysis requires electron microscope images of randomly oriented ice-embedded protein particles to be rotated and translated to allow for co...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2015.06.014

    authors： Zhao J,Brubaker MA,Benlekbir S,Rubinstein JL

    更新日期：2015-11-01 00:00:00

  • Crystal structure of the N-terminal RecA-like domain of a DEAD-box RNA helicase, the Dugesia japonica vasa-like gene B protein.

    abstract：:The Dugesia japonica vasa-like gene B (DjVLGB) protein is a DEAD-box RNA helicase of a planarian, which is well known for its strong regenerative capacity. DjVLGB shares sequence similarity to the Drosophila germ-line-specific DEAD-box RNA helicase Vasa, and even higher similarity to its paralogue, mouse PL10. In this...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2005.01.006

    authors： Kurimoto K,Muto Y,Obayashi N,Terada T,Shirouzu M,Yabuki T,Aoki M,Seki E,Matsuda T,Kigawa T,Okumura H,Tanaka A,Shibata N,Kashikawa M,Agata K,Yokoyama S

    更新日期：2005-04-01 00:00:00

  • A different conformation for linker L12 in IF molecules in the molecular and filamentous forms: an hypothesis.

    abstract：:The rod domain of IF molecules has been characterized as four alpha-helical coiled-coil segments (1A, 1B, 2A and 2B), three linkers (L1, L12 and L2) and a stutter at the centre of segment 2B. Two of these breaks in coiled-coil continuity (L2 and stutter) have been modelled on the basis of structural data obtained from...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.12.006

    authors： Parry DA,Smith TA

    更新日期：2010-05-01 00:00:00

  • Determining the mechanical properties of human corneal basement membranes with atomic force microscopy.

    abstract：:Biophysical cues such as substrate modulus have been shown to influence a variety of cell behaviors. We have determined the elastic modulus of the anterior basement membrane and Descemet's membrane of the human cornea with atomic force microscopy (AFM). A spherical probe was used with a radius approximating that of a ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.03.012

    authors： Last JA,Liliensiek SJ,Nealey PF,Murphy CJ

    更新日期：2009-07-01 00:00:00

  • An approach to automated acquisition of cryoEM images from lacey carbon grids.

    abstract：:An approach to automated acquisition of cryoEM image data from lacey carbon grids using the Leginon program is described. Automated liquid nitrogen top up of the specimen holder dewar was used as a step towards full automation, without operator intervention during the course of data collection. During cryoEM studies o...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2010.08.014

    authors： Nicholson WV,White H,Trinick J

    更新日期：2010-12-01 00:00:00

  • Structure-function studies of the magnetite-biomineralizing magnetosome-associated protein MamC.

    abstract：:Magnetotactic bacteria are Gram-negative bacteria that navigate along geomagnetic fields using the magnetosome, an organelle that consists of a membrane-enveloped magnetic nanoparticle. Magnetite formation and its properties are controlled by a specific set of proteins. MamC is a small magnetosome-membrane protein tha...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2016.03.001

    authors： Nudelman H,Valverde-Tercedor C,Kolusheva S,Perez Gonzalez T,Widdrat M,Grimberg N,Levi H,Nelkenbaum O,Davidov G,Faivre D,Jimenez-Lopez C,Zarivach R

    更新日期：2016-06-01 00:00:00

  • A 9 angstroms single particle reconstruction from CCD captured images on a 200 kV electron cryomicroscope.

    abstract：:Sub-nanometer resolution structure determination is becoming a common practice in electron cryomicroscopy of macromolecular assemblies. The data for these studies have until now been collected on photographic film. Using cytoplasmic polyhedrosis virus (CPV), a previously determined structure, as a test specimen, we sh...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2004.02.004

    authors： Booth CR,Jiang W,Baker ML,Zhou ZH,Ludtke SJ,Chiu W

    更新日期：2004-08-01 00:00:00

  • Crystal structure of MqnD (TTHA1568), a menaquinone biosynthetic enzyme from Thermus thermophilus HB8.

    abstract：:In many microorganisms, menaquinone is an essential lipid-soluble electron carrier. Recently, an alternative menaquinone biosynthetic pathway was found in some microorganisms [Hiratsuka, T., Furihata, K., Ishikawa, J., Yamashita, H., Itoh, N., Seto, H., Dairi, T., 2008. An alternative menaquinone biosynthetic pathway ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.07.007

    authors： Arai R,Murayama K,Uchikubo-Kamo T,Nishimoto M,Toyama M,Kuramitsu S,Terada T,Shirouzu M,Yokoyama S

    更新日期：2009-12-01 00:00:00

  • Cryoelectron microscopy of refrozen cryosections.

    abstract：:Cryoelectron microscopy makes it possible to record high-resolution detail from large and complex structures. However, its application to understanding cellular structure is limited by the requirement that samples should be no thicker than approximately 0.5-1 microm. Therefore it is important to develop the ability to...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/s1047-8477(03)00016-9

    authors： Luther PK,Morris EP

    更新日期：2003-05-01 00:00:00

  • Conical Fourier shell correlation applied to electron tomograms.

    abstract：:The resolution of electron tomograms is anisotropic due to geometrical constraints during data collection, such as the limited tilt range and single axis tilt series acquisition. Acquisition of dual axis tilt series can decrease these effects. However, in cryo-electron tomography, to limit the electron radiation damag...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2015.03.010

    authors： Diebolder CA,Faas FG,Koster AJ,Koning RI

    更新日期：2015-05-01 00:00:00

  • The crystallographic structure of Panicum Mosaic Virus (PMV).

    abstract：:The structure of Panicum Mosaic Virus (PMV) was determined by X-ray diffraction analysis to 2.9Å resolution. The crystals were of pseudo symmetry F23; the true crystallographic unit cell was of space group P2(1) with a=411.7Å, b=403.9Å and c=412.5Å, with β=89.7°. The asymmetric unit was two entire T=3 virus particles,...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2012.10.012

    authors： Makino DL,Larson SB,McPherson A

    更新日期：2013-01-01 00:00:00

  • Two different correlations between nanoindentation modulus and mineral content in the bone-cartilage interface.

    abstract：:The biomechanical properties of the zone of calcified cartilage (ZCC) in articulating joints are of clinical relevance due to the role ZCC plays in load transfer from cartilage to bone. To determine the micron-level mechanical properties and their correlation to mineral concentration in the ZCC, we combined nanoindent...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2004.10.010

    authors： Gupta HS,Schratter S,Tesch W,Roschger P,Berzlanovich A,Schoeberl T,Klaushofer K,Fratzl P

    更新日期：2005-02-01 00:00:00

  • Radial averages of astigmatic TEM images.

    abstract：:The Contrast Transfer Function (CTF) of an image, which modulates images taken from a Transmission Electron Microscope (TEM), is usually determined from the radial average of the power spectrum of the image (Frank, J., Three-dimensional Electron Microscopy of Macromolecular Assemblies, Oxford University Press, Oxford,...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2008.06.012

    authors： Fernando KV

    更新日期：2008-10-01 00:00:00