Abstract：
:Biophysical cues such as substrate modulus have been shown to influence a variety of cell behaviors. We have determined the elastic modulus of the anterior basement membrane and Descemet's membrane of the human cornea with atomic force microscopy (AFM). A spherical probe was used with a radius approximating that of a typical cell focal adhesion. Values obtained for the elastic modulus of the anterior basement membrane range from 2 to 15 kPa, with a mean of 7.5+/-4.2 kPa. The elastic modulus of Descemet's membrane was found to be slightly higher than those observed for the anterior basement membrane, with a mean of 50+/-17.8 kPa and a range of 20-80 kPa. The topography of Descemet's membrane has been shown to be similar to that of the anterior basement, but with smaller pore sizes resulting in a more tightly packed structure. This structural difference may account for the observed modulus differences. The determination of these values will allow for the design of a better model of the cellular environment as well as aid in the design and fabrication of artificial corneas.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Last JA,Liliensiek SJ,Nealey PF,Murphy CJdoi
10.1016/j.jsb.2009.03.012subject
Has Abstractpub_date
2009-07-01 00:00:00pages
19-24issue
1eissn
1047-8477issn
1095-8657pii
S1047-8477(09)00080-Xjournal_volume
167pub_type
杂志文章abstract：:Ion mobility mass spectrometry was employed to study the structure of the βB2B3-crystallin heterodimer following oxidation through its increased exposure to hydroxyl radicals. The results demonstrate that the heterodimer can withstand limited oxidation through the incorporation of up to some 10 oxygen atoms per subuni...
journal_title：Journal of structural biology
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journal_title：Journal of structural biology
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2016-09-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.1996.0049
更新日期：1996-03-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2009.02.007
更新日期：2009-05-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.1994.1004
更新日期：1994-01-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2009.10.007
更新日期：2010-03-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2010.06.022
更新日期：2010-12-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2016.06.024
更新日期：2017-02-01 00:00:00
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journal_title：Journal of structural biology
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2005-04-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2020.107447
更新日期：2020-03-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2011.05.017
更新日期：2011-09-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：1997-08-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.1999.4169
更新日期：1999-12-01 00:00:00
abstract：:DNA-dependent protein kinase (DNA-PK) is part of the eukaryotic DNA double strand break repair pathway and as such is crucial for maintenance of genomic stability, as well as for V(D)J (variable-diversity-joining) recombination. The catalytic subunit of DNA-PK (DNA-PKcs) belongs to the phosphatidylinositol-3 (PI-3) ki...
journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2004-03-01 00:00:00
