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Cellular electron cryo tomography and in situ sub-volume averaging reveal the context of microtubule-based processes.

Abstract：

:Electron cryo-tomography (cryoET) is currently the only technique that allows the direct observation of proteins in their native cellular environment. Sub-volume averaging of electron tomograms offers a route to increase the signal-to-noise of repetitive biological structures, such improving the information content and interpretability of tomograms. We discuss the potential for sub-volume averaging in highlighting and investigating specific processes in situ, focusing on microtubule structure and viral infection. We show that (i) in situ sub-volume averaging from single tomograms can guide and complement segmentation of biological features, (ii) the in situ determination of the structure of individual viruses is possible as they infect a cell, and (iii) novel, transient processes can be imaged with high levels of detail.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Grange M,Vasishtan D,Grünewald K

doi

10.1016/j.jsb.2016.06.024

subject

Has Abstract

pub_date

2017-02-01 00:00:00

pages

181-190

issue

2

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(16)30139-3

journal_volume

197

pub_type

杂志文章

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