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4.6A Cryo-EM reconstruction of tobacco mosaic virus from images recorded at 300 keV on a 4k x 4k CCD camera.

Abstract：

:Tobacco mosaic virus (TMV) is a plant virus with a highly ordered organisation and has been described in three different structural states: As stacked disks without RNA (X-ray crystallography), as a helical form with RNA (X-ray fibre diffraction) and as a second distinct helical form with RNA (cryo-EM). Here we present a structural analysis of TMV as a test object to assess the quality of cryo-EM images recorded at 300 keV on a CCD camera. The 4.6A TMV structure obtained is consistent with the previous cryo-EM structure and confirms that there is a second helical form of TMV. The structure here also shows that with a similar number of TMV segments an equivalent resolution can be achieved with a 4k CCD camera at 300 keV.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Clare DK,Orlova EV

doi

10.1016/j.jsb.2010.06.011

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

303-8

issue

3

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(10)00189-9

journal_volume

171

pub_type

杂志文章

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