Electron diffraction and high-resolution imaging on highly-crystalline β-chitin microfibril.

Abstract:

:The ultrastructure of β-chitin microfibrils from a centric diatom, Thalassiosira, and a tubeworm, Lamellibrachia, was studied using electron diffraction and high-resolution electron microscopy. Electron microdiffraction diagrams corresponding to each projection of the β-chitin crystals were obtained, and all the data support the structure model of anhydrous β-chitin crystals proposed by X-ray diffraction experiments. From high-resolution electron microscopy on ultrathin sections, the cross-sectional shapes of the microfibrils from Thalassiosira and Lamellibrachia were observed as a rectangular and parallelogram, respectively. The lattice fringes corresponding to the (010) plane of anhydrous β-chitin crystals were clearly observed in both cross-sections. Based on these observations, we have constructed a molecular packing model for β-chitin microfibrils.

journal_name

J Struct Biol

authors

Ogawa Y,Kimura S,Wada M

doi

10.1016/j.jsb.2011.07.001

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

83-90

issue

1

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(11)00194-8

journal_volume

176

pub_type

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