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Helical reconstruction in RELION.

Abstract：

:We describe a new implementation for the reconstruction of helical assemblies in the empirical Bayesian framework of RELION. Our approach calculates optimal linear filters for the 3D reconstruction by embedding helical symmetry operators in Fourier-space, and deals with deviations from perfect helical symmetry through Gaussian-shaped priors on the orientations of individual segments. By incorporating our approach into the standard pipeline for single-particle analysis in RELION, our implementation aims to be easily accessible for non-experienced users. Although our implementation does not solve the problem that grossly incorrect structures can be obtained when the wrong helical symmetry is imposed, we show for four different test cases that it is capable of reconstructing structures to near-atomic resolution.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

He S,Scheres SHW

doi

10.1016/j.jsb.2017.02.003

subject

Has Abstract

pub_date

2017-06-01 00:00:00

pages

163-176

issue

3

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(17)30019-9

journal_volume

198

pub_type

杂志文章

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