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Importance of detergent and phospholipid in the crystallization of the human erythrocyte anion-exchanger membrane domain.

Abstract：

:Three-dimensional crystals were obtained for the membrane domain of the human erythrocyte anion exchanger (AE1, Band 3). Protein homogeneity and stability and the delicate balance between the detergent used and the amount of phospholipids copurifying are critical to the formation of three-dimensional crystals of the AE1 membrane domain. While deglycosylation improved the protein homogeneity, its stability was significantly increased by inhibitor binding. Size-exclusion chromatography showed that the protein was monodisperse in detergents with acyl chains of 10-12 carbons over a pH range of 5.5-10.0. This pH range and the detergents that retained the protein's monodispersity were used for crystallization screening. Crystals were obtained with the protein purified in C(12)E(8), dodecylmaltoside, decylthiomaltoside, and cyclohexyl-hexylmaltoside. Five to 13 lipid molecules per protein were required for the protein crystal formation. Those crystals grown in dodecylmaltoside diffracted X-rays to 14 A. With these factors taken into consideration, ways to further improve the crystal quality are suggested.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Lemieux MJ,Reithmeier RA,Wang DN

doi

10.1016/s1047-8477(02)00010-2

keywords:

subject

Has Abstract

pub_date

2002-03-01 00:00:00

pages

322-32

issue

3

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(02)00010-2

journal_volume

137

pub_type

杂志文章

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