Crystallization and preliminary X-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina.

abstract：:Sickle hemoglobin (HbS) polymerization initiates in the deoxy state with the binding of hydrophobic patch formed by the isopropyl group of βVal6 residue of a hemoglobin tetramer with the hydrophobic pocket of another tetramer, whose hydrophobic patch binds to the hydrophobic groove of a third molecule. Subsequent elon...

journal_title：Journal of structural biology

authors： Das R,Mitra A,Bhat V,Mandal AK

更新日期：2017-07-01 00:00:00

abstract：:Cryo-tomography in the electron microscope is unique in its ability to provide high-resolution, three-dimensional structural information about cells, organelles and macromolecules in a nearly native, frozen-hydrated state. However, the phase-contrast imaging method used in conventional cryo-electron tomography fails t...

journal_title：Journal of structural biology

authors： Danev R,Kanamaru S,Marko M,Nagayama K

更新日期：2010-08-01 00:00:00

abstract：:Annexins are soluble cytosolic proteins that bind to cell membranes. Annexin A5 self-assembles into a two-dimensional (2D) array and prevents cell rupture by attaching to damaged membranes. However, this process is not fully understood at the molecular level. In this study, we determined the crystal structures of anne...

journal_title：Journal of structural biology

authors： Hong S,Na S,Kim OH,Jeong S,Oh BC,Ha NC

更新日期：2020-01-01 00:00:00

abstract：:G-protein coupled receptors (GPCRs) are one of the largest families of membrane receptors in eukaryotes. Heterotrimeric G-proteins, composed of α, β and γ subunits, are important molecular switches in the mediation of GPCR signaling. Receptor stimulation after the binding of a suitable ligand leads to G-protein hetero...

journal_title：Journal of structural biology

authors： Baltoumas FA,Theodoropoulou MC,Hamodrakas SJ

更新日期：2013-06-01 00:00:00

abstract：:The bacterial type II protein secretion (T2S) and type IV piliation (T4P) systems share several common features. In particular, it is well established that the T2S system requires the function of a pilus-like structure, called pseudopilus, which is built upon assembly of pilin-like subunits, called pseudopilins. Pilin...

journal_title：Journal of structural biology

authors： Alphonse S,Durand E,Douzi B,Waegele B,Darbon H,Filloux A,Voulhoux R,Bernard C

更新日期：2010-01-01 00:00:00

abstract：:Cryo-electron tomography of vitreous sections is currently the most promising technique for visualizing arbitrary regions of eukaryotic cells or tissue at molecular resolution. Despite significant progress in the sample preparation techniques over the past few years, the three dimensional reconstruction using electron...

journal_title：Journal of structural biology

authors： Castaño-Díez D,Al-Amoudi A,Glynn AM,Seybert A,Frangakis AS

更新日期：2008-03-01 00:00:00

abstract：:TDP-43 is a RNA/DNA-binding protein structurally related to nuclear hnRNP proteins. Previous biochemical studies have shown that this nuclear protein plays a role in the regulation of gene transcription, alternative splicing and mRNA stability. Despite the ubiquitous distribution of TDP-43, the growing list of TDP-43 ...

journal_title：Journal of structural biology

authors： Casafont I,Bengoechea R,Tapia O,Berciano MT,Lafarga M

更新日期：2009-09-01 00:00:00

abstract：:Single particle electron cryomicroscopy (cryo-EM) allows for structures of proteins and protein complexes to be determined from images of non-crystalline specimens. Cryo-EM data analysis requires electron microscope images of randomly oriented ice-embedded protein particles to be rotated and translated to allow for co...

journal_title：Journal of structural biology

authors： Zhao J,Brubaker MA,Benlekbir S,Rubinstein JL

更新日期：2015-11-01 00:00:00

abstract：:The structural remodeling of collagen is important in biological processes such as fibrosis, developmental morphogenesis and wound repair. Highly ordered collagen macromolecules produce second harmonic generation signals without the need for any exogenous label. Conversely, the cellular components stained with exogeno...

journal_title：Journal of structural biology

authors： Abraham T,Carthy J,McManus B

更新日期：2010-01-01 00:00:00

abstract：:The hypothesis that distinct chromatin domains expand and are remodelled differently when they undergo transcription, replication or cell cycle processes is well accepted. The condensation changes by which chromosomes are transformed at the metaphase-interphase transition are especially interesting and therefore exten...

journal_title：Journal of structural biology

authors： Batram C,Baddeley D,Kreth G,Cremer C

更新日期：2008-12-01 00:00:00

abstract：:In cryo-electron microscopy (cryo-EM) single-particle reconstruction, the heterogeneity of two-dimensional projection image data resulting from the co-existence of different conformational or ligand binding states of a macromolecular complex remains a major obstacle as it impairs the validity of reconstructed density ...

journal_title：Journal of structural biology

authors： Fu J,Gao H,Frank J

更新日期：2007-01-01 00:00:00

abstract：:Net-negatively-charged heterospecific A:B:C collagen peptide heterotrimers were designed using an automated computational approach. The design algorithm considers both target stability and the energy gap between the target states and misfolded competing states. Structural characterization indicates the net-negative ch...

journal_title：Journal of structural biology

authors： Parmar AS,Zahid S,Belure SV,Young R,Hasan N,Nanda V

更新日期：2014-02-01 00:00:00

abstract：:It has been postulated that phosphophoryn (PP) molecules bind specifically to type I collagen fibrils as the key event in inducing matrix mineralization in dentin. The nature and specificity of the collagen molecule-PP interaction has been examined by rotary shadowing-electron microscopy of mixtures of native, monomer...

journal_title：Journal of structural biology

authors： Dahl T,Sabsay B,Veis A

更新日期：1998-10-01 00:00:00

abstract：:Human scalp hairs are often examined microscopically to study the variation and diversity among a range of visible morphological traits. In this study, we focused on the ultrastructure of human scalp hair within its keratinized matrix, emphasizing, the density and distribution of melanosomes, variation in cuticle thic...

journal_title：Journal of structural biology

authors： Koch SL,Shriver MD,Jablonski NG

更新日期：2019-01-01 00:00:00

abstract：:In order to understand whole tooth behavior under load the biomechanical role of enamel and dentin has to be determined. We approach this question by comparing the deformation pattern and stiffness of intact teeth under load with the deformation pattern and stiffness of the same teeth after the enamel has been mechani...

journal_title：Journal of structural biology

authors： Barak MM,Geiger S,Chattah NL,Shahar R,Weiner S

更新日期：2009-12-01 00:00:00

abstract：:Processing of nuclear pre-mRNA is an important step in the regulation of gene expression and involves 5(')- and 3(')-end processing, splicing, and editing. Mammalian nuclear pre-mRNAs are assembled in large ribonucleoprotein (lnRNP) complexes, in which the entire population of nuclear pre-mRNA is individually packaged...

journal_title：Journal of structural biology

authors： Raitskin O,Angenitzki M,Sperling J,Sperling R

更新日期：2002-10-01 00:00:00

abstract：:The Caliciviridae is a family of nonenveloped, icosahedral, positive-sense single-stranded RNA viruses. This family of viruses consists of both animal and human pathogens. Adapting human caliciviruses to cell culture has not been successful, whereas some animal caliciviruses, including San Miguel sea lion virus, have ...

journal_title：Journal of structural biology

authors： Chen R,Neill JD,Prasad BV

更新日期：2003-02-01 00:00:00

abstract：:Although the fundamental steps in Fourier-based image analysis of electron micrographs of helical structures have not changed significantly since the techniques were developed 30 years ago, there have been developments which aid both the analysis itself and the interpretation of results. Increases in computational res...

journal_title：Journal of structural biology

authors： Owen CH,Morgan DG,DeRosier DJ

更新日期：1996-01-01 00:00:00

abstract：:Human alpha 2-macroglobulin (alpha 2M), a large tetrameric plasma glycoprotein, inhibits a wide spectrum of proteinases by a particular "trapping" mechanism resulting from the proteolysis of peptide bonds at specific "bait" regions. This induces the hydrolysis of four thiol esters triggering both the possible covalent...

journal_title：Journal of structural biology

authors： Boisset N,Taveau JC,Pochon F,Barray M,Delain E,Lamy JN

更新日期：1991-02-01 00:00:00

abstract：:We have cloned and expressed in Escherichia coli cDNA for rat nuclear transport factor 2 (NTF2), a key cytoplasmic factor that facilitates the import of proteins into the nucleus through nuclear pores. We have used this bacterially expressed material to produce orthorhombic crystals suitable for high-resolution X-ray ...

journal_title：Journal of structural biology

authors： Kent HM,Clarkson WD,Bullock TL,Stewart M

更新日期：1996-03-01 00:00:00

abstract：:The mature dentinoenamel junction (DEJ) is viewed by some investigators and the current authors, not as a fossilized, sharp transition between enamel and dentin, but as a relatively broad structural transition zone including the mantle dentin and the inner aprismatic enamel. In this study, the DEJ structure in bovine ...

journal_title：Journal of structural biology

authors： Stock SR,Vieira AE,Delbem AC,Cannon ML,Xiao X,Carlo FD

更新日期：2008-02-01 00:00:00

abstract：:It has been known since the 19th century that coral skeletons are built of aragonite crystals with taxonomy-linked arrangements, but the way by which each coral species controls this crystallization process remains an unsolved question. The problem became still more intriguing when it was shown that isotopic compositi...

journal_title：Journal of structural biology

authors： Cuif JP,Dauphin Y

更新日期：2005-06-01 00:00:00

abstract：:In recent years, cryo-electron microscopy (cryo-EM) has established itself as a key method in structural biology, permitting the structural characterization of large biomolecular complexes in various functional states. The data obtained through single-particle cryo-EM has recently seen a leap in resolution thanks to l...

journal_title：Journal of structural biology

authors： Trabuco LG,Schreiner E,Gumbart J,Hsin J,Villa E,Schulten K

更新日期：2011-03-01 00:00:00

abstract：:Brain deposition of Aβ in the form of amyloid plaques is a pathological hallmark of Alzheimer's disease. There are two major species of Aβ in the brain: Aβ42 and Aβ40. Although Aβ40 is several-fold more abundant than Aβ42 in soluble form, Aβ42 is the major component of amyloid plaques. Structural knowledge of Aβ42 fib...

journal_title：Journal of structural biology

authors： Gu L,Tran J,Jiang L,Guo Z

更新日期：2016-04-01 00:00:00

abstract：:Amyloid formation is associated with a range of debilitating human disorders including Alzheimer's and prion diseases. The amyloid structure is essential for understanding the role of amyloids in these diseases. Amyloid formation of Ure2 protein underlies the yeast prion [URE3]. Here we use site-directed spin labeling...

journal_title：Journal of structural biology

authors： Ngo S,Chiang V,Guo Z

更新日期：2012-11-01 00:00:00

abstract：:As a long-term goal we want to use cryoelectron tomography to understand how non-enveloped viruses, such as picornaviruses, enter cells and translocate their genomes across membranes. To this end, we developed new image-processing tools using an in vitro system to model viral interactions with membranes. The complex o...

journal_title：Journal of structural biology

authors： Bostina M,Bubeck D,Schwartz C,Nicastro D,Filman DJ,Hogle JM

更新日期：2007-11-01 00:00:00

abstract：:Chaperonins mediate protein folding in an ATP-dependent manner. ATP binding and hydrolysis by chaperonins are subject to both homotropic and heterotropic allosteric regulation. In the case of GroEL and CCT, homotropic regulation by ATP is manifested in nested cooperativity, which involves positive intra-ring cooperati...

journal_title：Journal of structural biology

authors： Horovitz A,Fridmann Y,Kafri G,Yifrach O

更新日期：2001-08-01 00:00:00

abstract：:Amyloid formation is a key pathological feature of Alzheimer's disease and is considered to be a major contributing factor to neurodegeneration and clinical dementia. Amyloid is found as both diffuse and senile plaques in the parenchyma of the brain and is composed primarily of the 40- to 42-residue amyloid-beta (Abet...

journal_title：Journal of structural biology

authors： McLaurin J,Yang D,Yip CM,Fraser PE

更新日期：2000-06-01 00:00:00

abstract：:BCP1 is a protein enriched in the nucleus that is required for Mss4 nuclear export and identified as the chaperone of ribosomal protein Rpl23 in Saccharomyces cerevisiae. According to sequence homology, BCP1 is related to the mammalian BRCA2-interacting protein BCCIP and belongs to the BCIP protein family (PF13862) in...

journal_title：Journal of structural biology

authors： Lin MH,Kuo PC,Chiu YC,Chang YY,Chen SC,Hsu CH

更新日期：2020-10-01 00:00:00

abstract：:Control of tissue composition and organization will be a key feature in the development of successful products through tissue engineering. However, the mechanism of collagen fibril formation, growth, and organization is not yet fully understood. In this study we have examined collagen fibril formation in a wound heali...

journal_title：Journal of structural biology

authors： White JF,Werkmeister JA,Darby IA,Bisucci T,Birk DE,Ramshaw JA

更新日期：2002-01-01 00:00:00