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A strategy for electron tomographic data collection and crystallographic reconstruction of biological bundles.

Abstract：

:Structures of highly ordered biological bundles have unique features which call for special experimental and computational methods in electron cryomicroscopy. They can be considered as three-dimensional quasi-crystals and reconstructed using a crystallographic approach. However, they are neither "infinitely" large with respect to the borders of the bundle, nor are they a single unit cell in thickness along the viewing direction. Also, because of their shape, bundles do not generally have a preferred azimuthal orientation, which poses challenges for orientation estimation and refinement. We developed a strategy for recording and processing electron cryomicroscopic images that differs from classical two-dimensional crystalline reconstruction techniques. These developments allowed us to merge data from tomographic tilt series of ice-embedded acrosomal bundles. The goal is to determine accurately amplitudes and phases at the diffraction maxima in terms of hkl indices, and compute a three-dimensional map from the diffraction data.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Sherman MB,Jakana J,Sun S,Matsudaira P,Chiu W,Schmid MF

doi

10.1006/jsbi.1997.3916

subject

Has Abstract

pub_date

1997-12-01 00:00:00

pages

245-56

issue

3

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(97)93916-2

journal_volume

120

pub_type

杂志文章

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