Abstract：
:Gap junctions containing extensive, highly ordered crystalline arrays of hexagonally packed connexons have been isolated from the hepatopancreas of the arthropod, Homarus americanus (American lobster). The structure of such junctions has been studied to a resolution of approximately 25 A in three dimensions by electron microscopy of negatively stained specimens. The structure, which has the crystallographic symmetry of the two-sided plane group p6, reveals the connexon as an annular oligomer which projects approximately 30-45 A from the cytoplasmic surface. The stain-filled channel structure appears to be approximately 40-45 A wide in the extracellular region. Projection images of glucose-embedded specimens extend to a resolution of 10 A, and show a strong contrast from the connexon subunits. Overall the structure is quite similar to that of rat liver junctions, except that less stain is seen in the aqueous region of the gap and more surrounding the protrusions of the protein into the cytoplasm.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Sikerwar SS,Downing KH,Glaeser RMdoi
10.1016/1047-8477(91)90075-8subject
Has Abstractpub_date
1991-06-01 00:00:00pages
255-63issue
3eissn
1047-8477issn
1095-8657pii
1047-8477(91)90075-8journal_volume
106pub_type
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