Efficient automatic noise reduction of electron tomographic reconstructions based on iterative median filtering.

abstract：:The optimal balance of cellular nucleotides and the efficient elimination of non-canonical nucleotides are critical to avoiding erroneous mutation during DNA replication. One such mechanism involves the degradation of excessive or abnormal nucleotides by nucleotide-hydrolyzing enzymes. YpgQ contains the histidine-aspa...

journal_title：Journal of structural biology

authors： Jeon YJ,Park SC,Song WS,Kim OH,Oh BC,Yoon SI

更新日期：2016-07-01 00:00:00

abstract：:This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

journal_title：Journal of structural biology

authors： Fan Y,Kong R,Tweardy DJ,Wong ST

更新日期：2013-02-18 00:00:00

abstract：:The exoskeleton of the American lobster Homarus americanus is a hierarchically organized nano-composite material consisting of organic chitin-protein fibers associated with inorganic calcium carbonate. The presence of a well-developed and periodically arranged pore canal system leads to a honeycomb-like structure. The...

journal_title：Journal of structural biology

authors： Sachs C,Fabritius H,Raabe D

更新日期：2008-02-01 00:00:00

abstract：:We have developed a simplified, efficient approach for the 3D reconstruction and analysis of mammalian cells in toto by electron microscope tomography (ET), to provide quantitative information regarding 'global' cellular organization at approximately 15-20 nm resolution. Two insulin-secreting beta cells-deemed 'functi...

journal_title：Journal of structural biology

authors： Noske AB,Costin AJ,Morgan GP,Marsh BJ

更新日期：2008-03-01 00:00:00

abstract：:Net-negatively-charged heterospecific A:B:C collagen peptide heterotrimers were designed using an automated computational approach. The design algorithm considers both target stability and the energy gap between the target states and misfolded competing states. Structural characterization indicates the net-negative ch...

journal_title：Journal of structural biology

authors： Parmar AS,Zahid S,Belure SV,Young R,Hasan N,Nanda V

更新日期：2014-02-01 00:00:00

abstract：:Ion-abrasion scanning electron microscopy (IASEM) takes advantage of focused ion beams to abrade thin sections from the surface of bulk specimens, coupled with SEM to image the surface of each section, enabling 3D reconstructions of subcellular architecture at approximately 30nm resolution. Here, we report the first a...

journal_title：Journal of structural biology

authors： Hildebrand M,Kim S,Shi D,Scott K,Subramaniam S

更新日期：2009-06-01 00:00:00

abstract：:In order to understand whole tooth behavior under load the biomechanical role of enamel and dentin has to be determined. We approach this question by comparing the deformation pattern and stiffness of intact teeth under load with the deformation pattern and stiffness of the same teeth after the enamel has been mechani...

journal_title：Journal of structural biology

authors： Barak MM,Geiger S,Chattah NL,Shahar R,Weiner S

更新日期：2009-12-01 00:00:00

abstract：:We analyzed a tertiary structure of systemin, the first identified polypeptide plant hormone, using two-dimensional NMR spectroscopy. From these data and molecular dynamics calculations we concluded that the peptide can adopt a Z-like-beta-sheet structure, which has previously been found in many specific DNA-binding p...

journal_title：Journal of structural biology

authors： Slósarek G,Kalbitzer HR,Mucha P,Rekowski P,Kupryszewski G,Giel-Pietraszuk M,Szymański M,Barciszewski J

更新日期：1995-07-01 00:00:00

abstract：:A common technique in transmission electron microscopy is the collection of a focal pair, in which the first, close to focus image contains higher resolution information at lower contrast, and the second, far from focus image has high contrast but less reliable high-resolution information. Typically these second micro...

journal_title：Journal of structural biology

authors： Ludtke SJ,Chiu W

更新日期：2003-10-01 00:00:00

abstract：:Overproduction and crystallization of Bacillus stearothermophilus ribosomal protein S7 (BstS7), a primary 16S rRNA binding protein and also a translational repressor protein, have been performed to analyze its three-dimensional structure by X-ray crystallography. Ribosomal protein BstS7 was expressed in the cytoplasmi...

journal_title：Journal of structural biology

authors： Harada N,Sano K,Kimura M,Hosaka H,Nakagawa A,Tanaka I

更新日期：1997-10-01 00:00:00

abstract：:DNA regions close to the origin of replication were visualized by the green fluorescent protein (GFP)-Lac repressor/lac operator system. The number of oriC-GFP fluorescent spots per cell and per nucleoid in batch-cultured cells corresponded to the theoretical DNA replication pattern. A similar pattern was observed in ...

journal_title：Journal of structural biology

authors： Elmore S,Müller M,Vischer N,Odijk T,Woldringh CL

更新日期：2005-09-01 00:00:00

abstract：:The nuclear arrangement of the ABL, c-MYC, and RB1 genes was quantitatively investigated in human undifferentiated HL-60 cells and in a terminally differentiated population of human granulocytes. The ABL gene was expressed in both cell types, the c-MYC gene was active in HL-60 cells and down-regulated in granulocytes,...

journal_title：Journal of structural biology

authors： Bártová E,Kozubek S,Jirsová P,Kozubek M,Gajová H,Lukásová E,Skalníková M,Ganová A,Koutná I,Hausmann M

更新日期：2002-08-01 00:00:00

abstract：:Native troponin-tropomyosin complex was isolated from Lethocerus indicus indirect flight muscle and tested for function. It was shown by rotary shadowing and by forming paracrystals on monolayers that the regulatory complex consists of a troponin head region approximately 130 A in diameter and a 400-A-long troponin T-...

journal_title：Journal of structural biology

authors： Wendt T,Guénebaut V,Leonard KR

更新日期：1997-02-01 00:00:00

abstract：:The acetyl-CoA decarbonylase/synthase (ACDS) complex is responsible for synthesis and cleavage of acetyl-CoA in methanogens. The complex is composed of five different subunits, with a probable stoichiometry of alpha(8)beta(8)gamma(8)delta(8)epsilon(8). The native molecular mass of a subcomponent of the ACDS complex fr...

journal_title：Journal of structural biology

authors： Kocsis E,Kessel M,DeMoll E,Grahame DA

更新日期：1999-12-15 00:00:00

abstract：:The architecture of the native human alpha 2-macroglobulin was studied by cryoelectron microscopy and image processing techniques. The lip, padlock, doughnut, and four-petaled flower views of this homotetrameric proteinase inhibitor were observed in the frozen-hydrated specimen, and a new view, termed eye view, was al...

journal_title：Journal of structural biology

authors： Larquet E,Boisset N,Pochon F,Lamy J

更新日期：1994-07-01 00:00:00

abstract：:Interdoublet sliding rates were assessed in bull sperm, utilizing a freeze-thaw procedure to allow axonemal disintegration. The sliding rate of 23 degrees C increased with increasing MgATP concentrations up to 1 mM ATP, to plateau at 8 microns/sec. The analyzed interdoublet shear in both live and demembranated (Triton...

journal_title：Journal of structural biology

authors： Bird Z,Hard R,Kanous KS,Lindemann CB

更新日期：1996-05-01 00:00:00

abstract：:TheL-A virus (LAV) particle is a specialized compartment for the transcription and replication of double-stranded RNA. It is 390 A in diameter and infects yeast. The particle is formed by a capsid containing 120 copies of a 680-residue gene product arranged with T = 1 icosahedral symmetry, approximately two copies of ...

journal_title：Journal of structural biology

authors： Naitow H,Canady MA,Lin T,Wickner RB,Johnson JE

更新日期：2001-07-01 00:00:00

abstract：:MDPP is a fully featured general-purpose image processing package primarily written to support research in structural biology using data gathered by electron microscopy. It has focused on the analysis of images using Fourier techniques, particularly periodic plane-layer and helical structures, but has extensive tools ...

journal_title：Journal of structural biology

authors： Smith PR,Gottesman SM

更新日期：1996-01-01 00:00:00

abstract：:COVID-19 pandemic, caused by SARS-CoV-2, has drastically affected human health all over the world. After the emergence of the pandemic the major focus of efforts to attenuate the infection has been on repurposing the already approved drugs to treat COVID-19 adopting a fast-track strategy. However, to date a specific r...

journal_title：Journal of structural biology

authors： Shahid M,Shahzad-Ul-Hussan S

更新日期：2020-12-29 00:00:00

abstract：:Gly missense mutations in type I collagen, which replace a conserved Gly in the repeating (Gly-Xaa-Yaa)n sequence with a larger residue, are known to cause Osteogenesis Imperfecta (OI). The clinical consequences of such mutations range from mild to lethal, with more serious clinical severity associated with larger Gly...

journal_title：Journal of structural biology

authors： Qiu Y,Mekkat A,Yu H,Yigit S,Hamaia S,Farndale RW,Kaplan DL,Lin YS,Brodsky B

更新日期：2018-09-01 00:00:00

abstract：:The collagen which forms the egg case of the dogfish, Scyliorhinus canicula, is assembled in a three-dimensional network that results in a very resistant capsule. The capsule presumably accomplishes both a protective and a filtering role for the embryo contained within it. In the present work we have obtained electron...

journal_title：Journal of structural biology

authors： Knupp C,Chew M,Squire J

更新日期：1998-01-01 00:00:00

abstract：:Here we introduce plusTipTracker, a Matlab-based open source software package that combines automated tracking, data analysis, and visualization tools for movies of fluorescently-labeled microtubule (MT) plus end binding proteins (+TIPs). Although +TIPs mark only phases of MT growth, the plusTipTracker software allows...

journal_title：Journal of structural biology

authors： Applegate KT,Besson S,Matov A,Bagonis MH,Jaqaman K,Danuser G

更新日期：2011-11-01 00:00:00

abstract：:The protist Trypanosoma brucei is an emerging model for the study of cilia and flagella. Here, we used scanning transmission electron microscopy (STEM) tomography to describe the structure of the trypanosome transition zone (TZ). At the base of the TZ, nine transition fibres irradiate from the B microtubule of each do...

journal_title：Journal of structural biology

authors： Trépout S,Tassin AM,Marco S,Bastin P

更新日期：2018-04-01 00:00:00

abstract：:Packaging of the Cystovirus varphi8 genome into the polymerase complex is catalysed by the hexameric P4 packaging motor. The motor is located at the fivefold vertices of the icosahedrally symmetric polymerase complex, and the symmetry mismatch between them may be critical for function. We have developed a novel image-...

journal_title：Journal of structural biology

authors： Huiskonen JT,Jäälinoja HT,Briggs JA,Fuller SD,Butcher SJ

更新日期：2007-05-01 00:00:00

abstract：:The protein tubulin is the main constituent of microtubules. Previous studies have shown that zinc ions induce the formation of crystalline sheets and macrotubes of tubulin. Both crystal types are suitable for structural studies by electron crystallography. However, crystallographic structural analysis of tubulin has ...

journal_title：Journal of structural biology

authors： Wolf SG,Mosser G,Downing KH

更新日期：1993-11-01 00:00:00

abstract：:Cryo-electron tomography (cryoET) allows 3D structural information to be obtained from cells and other biological samples in their close-to-native state. In combination with subtomogram averaging, detailed structures of repeating features can be resolved. CryoET data is collected as a series of images of the sample fr...

journal_title：Journal of structural biology

authors： Hagen WJH,Wan W,Briggs JAG

更新日期：2017-02-01 00:00:00

abstract：:The Contrast Transfer Function (CTF) of an image, which modulates images taken from a Transmission Electron Microscope (TEM), is usually determined from the radial average of the power spectrum of the image (Frank, J., Three-dimensional Electron Microscopy of Macromolecular Assemblies, Oxford University Press, Oxford,...

journal_title：Journal of structural biology

authors： Fernando KV

更新日期：2008-10-01 00:00:00

abstract：:Commonly employed data models for maximum likelihood refinement of electron microscopy images behave poorly in the presence of normalization errors. Small variations in background mean or signal brightness are relatively common in cryo-electron microscopy data, and varying signal-to-noise ratios or artifacts in the im...

journal_title：Journal of structural biology

authors： Scheres SH,Valle M,Grob P,Nogales E,Carazo JM

更新日期：2009-05-01 00:00:00

abstract：:Enameloid, the hyper-mineralized tissue covering shark teeth is a complex structure resulting from both ameloblast and odontoblast activity. The way these two types of cells interact to set up this tissue is not fully understood and results in the formation of subunits in the enameloid: the Single Crystallite Enameloi...

journal_title：Journal of structural biology

authors： Fellah C,Douillard T,Maire E,Meille S,Reynard B,Cuny G

更新日期：2020-11-19 00:00:00

abstract：:The mechanisms which cause macromolecules to form discrete compartments within the nucleus are not understood. Here, two ubiquitous compartments, nucleoli, and PML bodies, are shown to disassemble when K562 cell nuclei expand in medium of low monovalent cation concentration; their major proteins dispersed as seen by i...

journal_title：Journal of structural biology

authors： Hancock R

更新日期：2004-06-01 00:00:00