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Architecture of native human alpha 2-macroglobulin studied by cryoelectron microscopy and three-dimensional reconstruction.

Abstract：

:The architecture of the native human alpha 2-macroglobulin was studied by cryoelectron microscopy and image processing techniques. The lip, padlock, doughnut, and four-petaled flower views of this homotetrameric proteinase inhibitor were observed in the frozen-hydrated specimen, and a new view, termed eye view, was also characterized. The present three-dimensional reconstruction demonstrates that all these electron microscope views derive from a single three-dimensional structure. The molecule is composed of two horizontal bodies and of two oblique arches, which border a large central cavity. The polymorphism and the flexibility of the native alpha 2-macroglobulin are discussed.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Larquet E,Boisset N,Pochon F,Lamy J

doi

10.1006/jsbi.1994.1034

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

87-98

issue

1

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(84)71034-3

journal_volume

113

pub_type

杂志文章

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