当前位置： SCI文献检索 > JOURNAL OF STRUCTURAL BIOLOGY期刊下所有文献 > Transmission electron microscopy of the bacterial nucleoid.

Transmission electron microscopy of the bacterial nucleoid.

Abstract：

:Water-containing biological material cannot withstand the vacuum of the transmission electron microscope. The classical solution to this problem has been to dehydrate chemically fixed biological samples and then embed them in resin. During such treatment, the bacterial nucleoid is especially prone to aggregation, which affects its global shape and fine structure. Initial attempts to deal with aggregation by optimizing chemical fixation yielded contradictory results. Two decades ago, the situation improved with the introduction of freeze-substitution. This method is based on dehydration of unfixed cryo-immobilized samples at low temperature, which substantially reduces aggregation. As a result, the global shape of the nucleoid can be fairly well defined. Overall, in actively growing bacteria, the nucleoids are dispersed and "coralline" but become more confined when growth ceases. However, it is usually impossible to determine the molecular arrangement of DNA in the nucleoids of freeze-substituted bacteria because crystallization and the subsequent removal of water during substitution result in unavoidable distortions at the ultrastructural level. Recently, cryo-electron microscopy of vitreous sections has enabled the fully hydrated bacterial nucleoid to be studied close to the native state. Such studies have revealed aspects of bacterial nucleoid organization that are not preserved by freeze-substitution, including locally parallel or twisted bundles of DNA filaments, which are more frequently observed once bacterial growth has stopped, whereas in actively growing bacteria, the DNA is seen to be in a mostly disordered pattern.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Eltsov M,Zuber B

doi

10.1016/j.jsb.2006.07.007

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

246-54

issue

2

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(06)00215-2

journal_volume

156

pub_type

杂志文章,评审

相关文献

JOURNAL OF STRUCTURAL BIOLOGY文献大全
  • Structure of human DPEP3 in complex with the SC-003 antibody Fab fragment reveals basis for lack of dipeptidase activity.

    abstract：:Dipeptidase 3 (DPEP3) is one of three glycosylphosphatidylinositol-anchored metallopeptidases potentially involved in the hydrolytic metabolism of dipeptides. While its exact biological function is not clear, DPEP3 expression is normally limited to testis, but can be elevated in ovarian cancer. Antibody drug conjugate...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2020.107512

    authors： Hayashi K,Longenecker KL,Koenig P,Prashar A,Hampl J,Stoll V,Vivona S

    更新日期：2020-07-01 00:00:00

  • The promise of macromolecular crystallization in microfluidic chips.

    abstract：:Microfluidics, or lab-on-a-chip technology, is proving to be a powerful, rapid, and efficient approach to a wide variety of bioanalytical and microscale biopreparative needs. The low materials consumption, combined with the potential for packing a large number of experiments in a few cubic centimeters, makes it an att...

    journal_title：Journal of structural biology

    pub_type： 杂志文章,评审

    doi：10.1016/s1047-8477(03)00049-2

    authors： van der Woerd M,Ferree D,Pusey M

    更新日期：2003-04-01 00:00:00

  • Crystallographic Characterization of the DNA-Binding Domain of Interferon Regulatory Factor-2 Complexed with DNA.

    abstract：:Interferon regulatory factors (IRFs) are transcription factors for interferon-related genes, which manifest both antiviral and tumor-suppressor activities and regulate cell growth in response to DNA damage. For the transcription initiation of the interferon-beta gene, IRFs form a macromolecular assembly bound to the p...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1998.3970

    authors： Kusumoto M,Fujii Y,Tsukuda Y,Ohira T,Kyougoku Y,Taniguchi T,Hakoshima T

    更新日期：1998-01-01 00:00:00

  • External bone size identifies different strength-decline trajectories for the male human femora.

    abstract：:Understanding skeletal aging and predicting fracture risk is increasingly important with a growing elderly population. We hypothesized that when categorized by external bone size, the male femoral diaphysis would show different strength-age trajectories which can be explained by changes in morphology, composition and ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2020.107650

    authors： Bolger MW,Romanowicz GE,Bigelow EMR,Ward FS,Ciarelli A,Jepsen KJ,Kohn DH

    更新日期：2020-12-01 00:00:00

  • Cryo-negative staining reduces electron-beam sensitivity of vitrified biological particles.

    abstract：:Beam damage is the main resolution-limiting factor when biological particles are observed by cryoelectron microscopy in a thin vitrified solution film. Furthermore, the low contrast of the specimen frequently makes observation difficult and limits the possibility of image processing. Cryo-negative staining, in which t...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/s1047-8477(02)00035-7

    authors： De Carlo S,El-Bez C,Alvarez-Rúa C,Borge J,Dubochet J

    更新日期：2002-06-01 00:00:00

  • Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.

    abstract：:DivIVA or Wag31, which is an essential pole organizing protein in mycobacteria, can self-assemble at the negatively curved side of the membrane at the growing pole to form a higher order structural scaffold for maintaining cellular morphology and localizing various target proteins for cell-wall biogenesis. The structu...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2019.107429

    authors： Choukate K,Gupta A,Basu B,Virk K,Ganguli M,Chaudhuri B

    更新日期：2020-02-01 00:00:00

  • Exaptation of two ancient immune proteins into a new dimeric pore-forming toxin in snails.

    abstract：:The Membrane Attack Complex-Perforin (MACPF) family is ubiquitously found in all kingdoms. They have diverse cellular roles, however MACPFs with pore-forming toxic function in venoms and poisons are very rare in animals. Here we present the structure of PmPV2, a MACPF toxin from the poisonous apple snail eggs, that ca...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2020.107531

    authors： Giglio ML,Ituarte S,Milesi V,Dreon MS,Brola TR,Caramelo J,Ip JCH,Maté S,Qiu JW,Otero LH,Heras H

    更新日期：2020-08-01 00:00:00

  • Rapid near-atomic resolution single-particle 3D reconstruction with SIMPLE.

    abstract：:Cryogenic electron microscopy (cryo-EM) and single-particle analysis enables determination of near-atomic resolution structures of biological molecules. However, large computational requirements limit throughput and rapid testing of new image processing tools. We developed PRIME, an algorithm part of the SIMPLE softwa...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.08.005

    authors： Reboul CF,Kiesewetter S,Eager M,Belousoff M,Cui T,De Sterck H,Elmlund D,Elmlund H

    更新日期：2018-11-01 00:00:00

  • The structural basis of the low catalytic activities of the two minor β-carbonic anhydrases of the filamentous fungus Aspergillus fumigatus.

    abstract：:The β-carbonic anhydrases (β-CAs) are widely distributed zinc-metalloenzymes that play essential roles in growth, survival, development and virulence in fungi. The majority of filamentous ascomycetes possess multiple β-CA isoforms among which major and minor forms have been characterized. We examined the catalytic beh...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2019.07.011

    authors： Kim S,Kim NJ,Hong S,Kim S,Sung J,Jin MS

    更新日期：2019-10-01 00:00:00

  • Mechanical properties of cardiac titin's N2B-region by single-molecule atomic force spectroscopy.

    abstract：:Titin is a giant protein responsible for passive-tension generation in muscle sarcomeres. Here, we used single-molecule AFM force spectroscopy to investigate the mechanical characteristics of a recombinant construct from the human cardiac-specific N2B-region, which harbors a 572-residue unique sequence flanked by two ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2006.02.017

    authors： Leake MC,Grützner A,Krüger M,Linke WA

    更新日期：2006-08-01 00:00:00

  • Purification, crystallization, and preliminary X-ray diffraction analysis of the Tricorn protease hexamer from Thermoplasma acidophilum.

    abstract：:Tricorn protease from Thermoplasma acidophilum is a hexameric enzyme; in vivo the hexamers assemble further to form large icosahedral capsids of 14.6 MDa. Recombinant Tricorn protease was purified as an enzymatically active hexamer of 0.72 MDa that formed crystals of octahedral morphology under low-ionic-strength cond...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.2001.4360

    authors： Bosch J,Tamura T,Bourenkov G,Baumeister W,Essen LO

    更新日期：2001-04-01 00:00:00

  • How does osteocalcin lacking γ-glutamic groups affect biomimetic apatite formation and what can we say about its structure in mineral-bound form?

    abstract：:Non-collagenous proteins such as osteocalcin function as regulators of the mineralization process in bone. Osteocalcin undergoes post-translational modification adding an extra carboxylate group on three of its glutamate residues to enhance interaction with bone mineral. In this work, we examine regulation of biomimet...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2019.04.014

    authors： Iline-Vul T,Kulpanovich A,Nadav-Tsubery M,Semionov A,Keinan-Adamsky K,Goobes G

    更新日期：2019-08-01 00:00:00

  • Dry and survive: morphological changes during anhydrobiosis in a bdelloid rotifer.

    abstract：:Bdelloid rotifers are aquatic microinvertebrates able to cope with the loss of environmental water by entering dormancy, and are thus capable of living in temporary habitats. When water is evaporating, bdelloids contract into "tuns", silence metabolism and lose water from the body, a condition known as anhydrobiosis. ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2010.04.003

    authors： Marotta R,Leasi F,Uggetti A,Ricci C,Melone G

    更新日期：2010-07-01 00:00:00

  • Single particle reconstruction and validation using Bsoft for the map challenge.

    abstract：:The Bsoft package is aimed at processing electron micrographs for the determination of the three-dimensional structures of biological specimens. Recent advances in hardware allow us to solve structures to near atomic resolution using single particle analysis (SPA). The Map Challenge offered me an opportunity to test t...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.07.003

    authors： Heymann JB

    更新日期：2018-10-01 00:00:00

  • Stabilizing role of glutamic acid 222 in the structure of Enhanced Green Fluorescent Protein.

    abstract：:Enhanced Green Fluorescent Protein (EGFP) is a variant of wild-type Green Fluorescent Protein from the jellyfish Aequorea victoria, whose mutations S65T and F64L increase brightness and folding efficiency. EGFP is extensively used in cell biology and biochemistry as a colocalization or expression reporter. Surprisingl...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2011.02.004

    authors： Royant A,Noirclerc-Savoye M

    更新日期：2011-05-01 00:00:00

  • Micro-structure and chemical composition of vateritic deformities occurring in the bivalve Corbicula fluminea (Müller, 1774).

    abstract：:Vateritic deformities occurring in the invasive heterodont bivalve Corbicula fluminea from several locations in the UK were characterised in detail for the first time using scanning electron microscopy, X-ray diffraction and different geochemical techniques (electron microprobe, ICP-AES, and mass spectrometry). Large ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2011.02.002

    authors： Frenzel M,Harper EM

    更新日期：2011-05-01 00:00:00

  • Microstructures of external periosteal callus of repaired femoral fracture in children.

    abstract：:In order to understand further the mechanism of bone fracture repair, and thus to innovate better operative treatment for bone fracture and to design new implant materials for bone repair, microstructures of external periosteal callus (EPC) of repaired femoral fracture in both children and adults were investigated by ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1996.0084

    authors： Cui FZ,Wen HB,Su XW,Zhu XD

    更新日期：1996-11-01 00:00:00

  • Structure of the Pseudomonas aeruginosa XcpT pseudopilin, a major component of the type II secretion system.

    abstract：:The bacterial type II protein secretion (T2S) and type IV piliation (T4P) systems share several common features. In particular, it is well established that the T2S system requires the function of a pilus-like structure, called pseudopilus, which is built upon assembly of pilin-like subunits, called pseudopilins. Pilin...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.09.003

    authors： Alphonse S,Durand E,Douzi B,Waegele B,Darbon H,Filloux A,Voulhoux R,Bernard C

    更新日期：2010-01-01 00:00:00

  • Three-dimensional reconstruction with contrast transfer function correction from energy-filtered cryoelectron micrographs: procedure and application to the 70S Escherichia coli ribosome.

    abstract：:Cryoelectron microscopy provides the means of studying macromolecules in their native state. However, the contrast transfer function (CTF) makes the images and the three-dimensional (3D) maps derived from them difficult to interpret. We developed methods to determine the CTF from experimental data and to obtain a CTF-...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1997.3845

    authors： Zhu J,Penczek PA,Schröder R,Frank J

    更新日期：1997-04-01 00:00:00

  • The three-dimensional structure of frozen-hydrated Nudaurelia capensis beta virus, a T = 4 insect virus.

    abstract：:The three-dimensional structure of Nudaurelia capensis beta virus (N beta V) was reconstructed to 3.2-nm resolution from images of frozen-hydrated virions. The distinctly icosahedral capsid (approximately 40-nm diameter) contains 240 copies of a single 61-kDa protein subunit arranged with T = 4 lattice symmetry. The o...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/1047-8477(90)90105-l

    authors： Olson NH,Baker TS,Johnson JE,Hendry DA

    更新日期：1990-10-01 00:00:00

  • The combination of chemical fixation procedures with high pressure freezing and freeze substitution preserves highly labile tissue ultrastructure for electron tomography applications.

    abstract：:The emergence of electron tomography as a tool for three dimensional structure determination of cells and tissues has brought its own challenges for the preparation of thick sections. High pressure freezing in combination with freeze substitution provides the best method for obtaining the largest volume of well-preser...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2007.09.002

    authors： Sosinsky GE,Crum J,Jones YZ,Lanman J,Smarr B,Terada M,Martone ME,Deerinck TJ,Johnson JE,Ellisman MH

    更新日期：2008-03-01 00:00:00

  • Ultrastructure of familial amyloid polyneuropathy amyloid fibrils: examination with high-resolution electron microscopy.

    abstract：:The ultrastructure of familial amyloid polyneuropathy (FAP) amyloid fibrils was examined with high-resolution electron microscopy and immunolabeling. Sural nerve biopsies from FAP (Met 30) patients as well as control tissues were prepared for thin-section observations. Extracellular spaces in the vicinity of myelinate...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1998.4052

    authors： Inoue S,Kuroiwa M,Saraiva MJ,Guimarães A,Kisilevsky R

    更新日期：1998-12-01 00:00:00

  • Strategies for the crystallization of viruses: using phase diagrams and gels to produce 3D crystals of Grapevine fanleaf virus.

    abstract：:The small icosahedral plant RNA nepovirus Grapevine fanleaf virus (GFLV) is specifically transmitted by a nematode and causes major damage to vineyards worldwide. To elucidate the molecular mechanisms underlying the recognition between the surface of its protein capsid and cellular components of its vector, host and v...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2011.02.007

    authors： Schellenberger P,Demangeat G,Lemaire O,Ritzenthaler C,Bergdoll M,Oliéric V,Sauter C,Lorber B

    更新日期：2011-05-01 00:00:00

  • Crystallization and preliminary crystallographic analysis of San Miguel sea lion virus: an animal calicivirus.

    abstract：:The Caliciviridae is a family of nonenveloped, icosahedral, positive-sense single-stranded RNA viruses. This family of viruses consists of both animal and human pathogens. Adapting human caliciviruses to cell culture has not been successful, whereas some animal caliciviruses, including San Miguel sea lion virus, have ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/s1047-8477(02)00583-x

    authors： Chen R,Neill JD,Prasad BV

    更新日期：2003-02-01 00:00:00

  • Evidence for local 32 symmetry in homotrimeric riboflavin synthase of Escherichia coli.

    abstract：:Riboflavin synthase is a trimer of identical 23-kDa subunits. The primary structure is characterized by considerable similarity of the C-terminal and N-terminal parts. Recombinant riboflavin synthase of Escherichia coli and Bacillus subtilis was crystallized by the vapor diffusion method. Crystals of E. coli riboflavi...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1997.3935

    authors： Meining W,Tibbelin G,Ladenstein R,Eberhardt S,Fischer M,Bacher A

    更新日期：1998-01-01 00:00:00

  • STEM tomography analysis of the trypanosome transition zone.

    abstract：:The protist Trypanosoma brucei is an emerging model for the study of cilia and flagella. Here, we used scanning transmission electron microscopy (STEM) tomography to describe the structure of the trypanosome transition zone (TZ). At the base of the TZ, nine transition fibres irradiate from the B microtubule of each do...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2017.12.005

    authors： Trépout S,Tassin AM,Marco S,Bastin P

    更新日期：2018-04-01 00:00:00

  • AVS software for visualization in molecular microscopy.

    abstract：:AVS (Application Visualization System) is commercially available software for analyzing and viewing data. AVS is primarily used in the physical sciences and engineering, and here we describe the application of AVS for examining three-dimensional density maps generated by electron microscopy and image processing. For t...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1996.0017

    authors： Sheehan B,Fuller SD,Pique ME,Yeager M

    更新日期：1996-01-01 00:00:00

  • The glucocorticoid receptor in homodimeric and monomeric form visualised by electron microscopy.

    abstract：:The purified glucocorticoid receptor (GR) from rat liver has been visualised by electron microscopy. The specimens were prepared by spreading on thin carbon support and negatively stained using uranyl acetate. Two forms of GR, the monomeric and the dimeric forms, were identified based on size, chromatographic distribu...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/1047-8477(91)90030-z

    authors： Eriksson P,Daneholt B,Wrange O

    更新日期：1991-08-01 00:00:00

  • Improved applicability and robustness of fast cryo-electron tomography data acquisition.

    abstract：:The power of cryo-electron tomography (cryoET) lies in its capability to characterize macromolecules in their cellular context. Structure determination by cryoET, however, is time-consuming compared to single particle approaches. A recent study reported significant acceleration of data acquisition by a fast-incrementa...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2019.08.006

    authors： Eisenstein F,Danev R,Pilhofer M

    更新日期：2019-11-01 00:00:00

  • IRIS explorer software for radial-depth cueing reovirus particles and other macromolecular structures determined by cryoelectron microscopy and image reconstruction.

    abstract：:Structures of biological macromolecules determined by transmission cryoelectron microscopy (cryo-TEM) and three-dimensional image reconstruction are often displayed as surface-shaded representations with depth cueing along the viewed direction (Z cueing). Depth cueing to indicate distance from the center of virus part...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1997.3902

    authors： Spencer SM,Sgro JY,Dryden KA,Baker TS,Nibert ML

    更新日期：1997-10-01 00:00:00