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Analysis of immunolabeled cells by atomic force microscopy, optical microscopy, and flow cytometry.

Abstract：

:In this study we investigated the applicability of the (silver-enhanced) immunogold labeling method for atomic force microscopy. Human lymphocytes were labeled with anti-CD3 conjugated to fluorescein isothiocyanate and a secondary antibody (goat anti-mouse) linked with 1- or 30-nm colloidal gold particles. Silver enhancement was applied on these labeled cells to increase the size of the labels. In a setup combining an inverted optical microscope and a stand-alone atomic force microscope, a direct correlation was made between the force and the fluorescent images. Additionally, we performed flow cytometric analysis. From the results we conclude that immunogold labeling using small labels (1 nm) in combination with silver enhancement (30 min) proves to be a reliable method for high-resolution cell surface antigen detection in atomic force microscopy.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Neagu C,van der Werf KO,Putman CA,Kraan YM,de Grooth BG,van Hulst NF,Greve J

doi

10.1006/jsbi.1994.1004

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

32-40

issue

1

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(84)71004-5

journal_volume

112

pub_type

杂志文章

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