Abstract：
:Integral membrane proteins are involved in virtually every cellular process. Precisely regulating these machineries would allow controlling many human and vertebrate diseases. Embedded into cellular membranes, membrane proteins establish molecular interactions that sensitively react to environmental changes and to molecular compounds, such as ligands or inhibitors. We applied atomic force microscopy (AFM) to image the Na(+)/H(+) antiporter MjNhaP1 from Methanococcus jannaschii, and single-molecule force spectroscopy (SMFS) to probe molecular interactions that drive the protein structure-function relationship. High-resolution AFM topographs showed the dimeric assembly of MjNhaP1 being reconstituted into a lipid bilayer. SMFS of MjNhaP1 unraveled molecular interactions stabilizing individual structural domains. Transmembrane domains exhibited certain probabilities to unfold individually or cooperatively with other domains resulting in different unfolding pathways. Helices VIII and X established pH sensitive interactions altering significantly upon MjNhaP1 activation, while removal of the ligand (Na(+)) destabilized the entire antiporter except helix VIII. It is assumed that Asp234/235 of helix VIII are involved in the ligand-binding site and that helix X plays a functional role in the activation of the transporter.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Kedrov A,Wegmann S,Smits SH,Goswami P,Baumann H,Muller DJdoi
10.1016/j.jsb.2007.02.010subject
Has Abstractpub_date
2007-08-01 00:00:00pages
290-301issue
2eissn
1047-8477issn
1095-8657pii
S1047-8477(07)00057-3journal_volume
159pub_type
杂志文章abstract：:Three questions central to understanding the initiation of DNA replication in eukaryotes are: (1) Does DNA synthesis begin at a defined place? (2) What determines replication initiation sites? (3) What regulates an origin to fire only once per cell cycle? A key player in this is the origin recognition complex (ORC), r...
journal_title：Journal of structural biology
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journal_title：Journal of structural biology
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journal_title：Journal of structural biology
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journal_title：Journal of structural biology
pub_type： 杂志文章
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2010-09-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.2001.4343
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2016.04.007
更新日期：2016-06-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2010.01.013
更新日期：2010-04-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.2001.4415
更新日期：2001-09-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2008.12.001
更新日期：2009-04-01 00:00:00
abstract：:The assembly of the collagenous organic matrix prior to mineralization is a key step in the formation of bones and teeth. This process was studied in the predentin of continuously forming rat incisors, using unstained vitrified ice sections examined in the transmission electron microscope. Progressing from the odontob...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.2000.4320
更新日期：2000-12-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/1047-8477(91)90060-a
更新日期：1991-02-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：1999-06-30 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2017.02.003
更新日期：2017-06-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2005-05-01 00:00:00
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journal_title：Journal of structural biology
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更新日期：2003-05-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2007-05-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2017.06.003
更新日期：2017-12-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2020-11-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
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更新日期：2020-11-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章,评审
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更新日期：1999-04-01 00:00:00
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journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1006/jsbi.1997.3902
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abstract：:Charge contrast imaging (CCI) is a dynamic phenomenon recently reported in insulating and semiconducting materials imaged with low vacuum or variable pressure scanning electron microscopes (SEM). Data presented in this paper illustrates that CCI can also be applied to biominerals and biological soft-tissues and that u...
journal_title：Journal of structural biology
pub_type： 杂志文章
doi：10.1016/j.jsb.2006.04.004
更新日期：2006-09-01 00:00:00
