Actin filament tracking in electron tomograms of negatively stained lamellipodia using the localized radon transform.

abstract：:It has been postulated that phosphophoryn (PP) molecules bind specifically to type I collagen fibrils as the key event in inducing matrix mineralization in dentin. The nature and specificity of the collagen molecule-PP interaction has been examined by rotary shadowing-electron microscopy of mixtures of native, monomer...

journal_title：Journal of structural biology

authors： Dahl T,Sabsay B,Veis A

更新日期：1998-10-01 00:00:00

abstract：:Different types of cells have been imaged with the atomic force microscope. The morphology of the archaebacterium Halobacterium halobium in its dry state was revealed. On a leaf of the small Indian tree Lagerstroemia subcostata a stoma was imaged. The lower side of a water lily leaf was imaged in water showing feature...

journal_title：Journal of structural biology

authors： Butt HJ,Wolff EK,Gould SA,Dixon Northern B,Peterson CM,Hansma PK

更新日期：1990-10-01 00:00:00

abstract：:Long-depth imaging of specific gene expression in the midgestation whole-mount mouse embryo (WME) is demonstrated with single-photon excitation (1PE) confocal fluorescence microscopy and fluorescence in situ hybridization. Expression domains of Pax-6 mRNA transcripts were labeled with an in situ hybridization probe th...

journal_title：Journal of structural biology

authors： Palmes-Saloma C,Saloma C

更新日期：2000-07-01 00:00:00

abstract：:The use of electron tomography has allowed the three-dimensional membrane topography of the mitochondrion to be better understood. The most striking feature of this topology is the crista junction, a structure that may serve to divide functionally the inner membrane and intermembrane spaces. In situ these junctions se...

journal_title：Journal of structural biology

authors： Renken C,Siragusa G,Perkins G,Washington L,Nulton J,Salamon P,Frey TG

更新日期：2002-04-01 00:00:00

abstract：:The structural remodeling of collagen is important in biological processes such as fibrosis, developmental morphogenesis and wound repair. Highly ordered collagen macromolecules produce second harmonic generation signals without the need for any exogenous label. Conversely, the cellular components stained with exogeno...

journal_title：Journal of structural biology

authors： Abraham T,Carthy J,McManus B

更新日期：2010-01-01 00:00:00

abstract：:Interdoublet sliding rates were assessed in bull sperm, utilizing a freeze-thaw procedure to allow axonemal disintegration. The sliding rate of 23 degrees C increased with increasing MgATP concentrations up to 1 mM ATP, to plateau at 8 microns/sec. The analyzed interdoublet shear in both live and demembranated (Triton...

journal_title：Journal of structural biology

authors： Bird Z,Hard R,Kanous KS,Lindemann CB

更新日期：1996-05-01 00:00:00

abstract：:The crystal structure of the N-terminal domain of the RNA polymerase δ subunit (Nδ) from Bacillus subtilis solved at a resolution of 2.0Å is compared with the NMR structure determined previously. The molecule crystallizes in the space group C222(1) with a dimer in the asymmetric unit. Importantly, the X-ray structure ...

journal_title：Journal of structural biology

authors： Demo G,Papoušková V,Komárek J,Kadeřávek P,Otrusinová O,Srb P,Rabatinová A,Krásný L,Žídek L,Sklenář V,Wimmerová M

更新日期：2014-08-01 00:00:00

abstract：:Three questions central to understanding the initiation of DNA replication in eukaryotes are: (1) Does DNA synthesis begin at a defined place? (2) What determines replication initiation sites? (3) What regulates an origin to fire only once per cell cycle? A key player in this is the origin recognition complex (ORC), r...

journal_title：Journal of structural biology

authors： Gerbi SA,Strezoska Z,Waggener JM

更新日期：2002-10-01 00:00:00

abstract：:Trypanosoma cruzi chagasin belongs to a recently discovered family of cysteine protease inhibitors found in lower eukaryotes and prokaryotes but not in mammals. Chagasin binds tightly to cruzain, the major lysosomal T. cruzi cysteine protease, involved with infectivity and survival of the parasite in mammalian host ce...

journal_title：Journal of structural biology

authors： Figueiredo da Silva AA,de Carvalho Vieira L,Krieger MA,Goldenberg S,Zanchin NI,Guimarães BG

更新日期：2007-02-01 00:00:00

abstract：:A random conical tilt reconstruction of negatively stained Saccharomyces cerevisiae fatty acid synthase was used as a model to compute a three-dimensional reconstruction from untilted stain specimens of the molecules in multiple orientations using a three-dimensional projection alignment method. The resulting structur...

journal_title：Journal of structural biology

authors： Kolodziej SJ,Penczek PA,Stoops JK

更新日期：1997-11-01 00:00:00

abstract：:Random spherically constrained (RSC) single particle reconstruction is a method to obtain structures of membrane proteins embedded in lipid vesicles (liposomes). As in all single-particle cryo-EM methods, structure determination is greatly aided by reliable detection of protein "particles" in micrographs. After fittin...

journal_title：Journal of structural biology

authors： Liu Y,Sigworth FJ

更新日期：2014-03-01 00:00:00

abstract：:We describe here a set of multiresolution visualization and docking procedures that we refer to as the Situs package. The package was developed to provide an efficient and robust method for the fitting of atomic structures into low-resolution data. The current release was optimized specifically for the visualization a...

journal_title：Journal of structural biology

authors： Wriggers W,Birmanns S

更新日期：2001-02-01 00:00:00

abstract：:Zinc-induced sheets of tubulin are two-dimensional crystalline polymers that constitute an ideal sample for high resolution studies of tubulin by electron crystallography. We show that these 2-dimensional tubulin crystals can be stabilized by taxol against low-temperature depolymerization and degradation with time, ea...

journal_title：Journal of structural biology

authors： Nogales E,Wolf SG,Zhang SX,Downing KH

更新日期：1995-09-01 00:00:00

abstract：:The exoskeleton of the American lobster Homarus americanus is a hierarchically organized nano-composite material consisting of organic chitin-protein fibers associated with inorganic calcium carbonate. The presence of a well-developed and periodically arranged pore canal system leads to a honeycomb-like structure. The...

journal_title：Journal of structural biology

authors： Sachs C,Fabritius H,Raabe D

更新日期：2008-02-01 00:00:00

abstract：:Tomo3D is a program for fast tomographic reconstruction on multicore computers. Its high speed stems from code optimization, vectorization with Streaming SIMD Extensions (SSE), multithreading and optimization of disk access. Recently, Advanced Vector eXtensions (AVX) have been introduced in the x86 processor architect...

journal_title：Journal of structural biology

authors： Agulleiro JI,Fernandez JJ

更新日期：2015-02-01 00:00:00

abstract：:The assembly of the collagenous organic matrix prior to mineralization is a key step in the formation of bones and teeth. This process was studied in the predentin of continuously forming rat incisors, using unstained vitrified ice sections examined in the transmission electron microscope. Progressing from the odontob...

journal_title：Journal of structural biology

authors： Beniash E,Traub W,Veis A,Weiner S

更新日期：2000-12-01 00:00:00

abstract：:The genomic DNA in cells of Escherichia coli is localized in one or two compact, phase-like regions with characteristic shapes. Nucleoids undergo progressive changes in shape and compaction in the presence of drugs such as chloramphenicol or puromycin. Forces which influence nucleoid shape and compaction are reviewed,...

journal_title：Journal of structural biology

authors： Zimmerman SB

更新日期：2006-11-01 00:00:00

abstract：:The nuclear arrangement of the ABL, c-MYC, and RB1 genes was quantitatively investigated in human undifferentiated HL-60 cells and in a terminally differentiated population of human granulocytes. The ABL gene was expressed in both cell types, the c-MYC gene was active in HL-60 cells and down-regulated in granulocytes,...

journal_title：Journal of structural biology

authors： Bártová E,Kozubek S,Jirsová P,Kozubek M,Gajová H,Lukásová E,Skalníková M,Ganová A,Koutná I,Hausmann M

更新日期：2002-08-01 00:00:00

abstract：:Amelogenin is believed to be involved in controlling the formation of the highly anisotropic and ordered hydroxyapatite crystallites that form enamel. The adsorption behavior of amelogenin proteins onto substrates is very important because protein-surface interactions are critical to its function. We have previously u...

journal_title：Journal of structural biology

authors： Tarasevich BJ,Lea S,Shaw WJ

更新日期：2010-03-01 00:00:00

abstract：:The collagen which forms the egg case of the dogfish, Scyliorhinus canicula, is assembled in a three-dimensional network that results in a very resistant capsule. The capsule presumably accomplishes both a protective and a filtering role for the embryo contained within it. In the present work we have obtained electron...

journal_title：Journal of structural biology

authors： Knupp C,Chew M,Squire J

更新日期：1998-01-01 00:00:00

abstract：:The structure of full-length human TLR5 determined by electron microscopy single-particle image reconstruction at 26Å resolution shows that TLR5 forms an asymmetric homodimer via ectodomain interactions. The structure shows that like TLR9, TLR5 dimerizes in the absence of ligand. The asymmetry of the dimer suggests th...

journal_title：Journal of structural biology

authors： Zhou K,Kanai R,Lee P,Wang HW,Modis Y

更新日期：2012-02-01 00:00:00

abstract：:Nanoparticle transport across cell membrane plays a crucial role in the development of drug delivery systems as well as in the toxicity response induced by nanoparticles. As hydrophilic nanoparticles interact with lipid membranes and are able to induce membrane perturbations, hypothetic mechanisms based on membrane cu...

journal_title：Journal of structural biology

authors： Le Bihan O,Bonnafous P,Marak L,Bickel T,Trépout S,Mornet S,De Haas F,Talbot H,Taveau JC,Lambert O

更新日期：2009-12-01 00:00:00

abstract：:Nearly a third of all eukaryotic proteins are transported from the ER to the Golgi apparatus through the secretory pathway using COPII coated vesicles. Evidence suggests that this transport occurs via 500-900 Å vesicles that bud from the ER membrane. It has been shown that procollagen molecules utilize the COPII prote...

journal_title：Journal of structural biology

authors： O'Donnell J,Maddox K,Stagg S

更新日期：2011-02-01 00:00:00

abstract：:Mononuclear macrophages are important immune cells in the organisms. The complicated membrane structure underlying the diverse functions of mononuclear-macrophage has been largely unresolved. As a representative of monocyte-derived macrophages, the membrane structure of PMA differentiated THP-1 cells was comprehensive...

journal_title：Journal of structural biology

authors： Tian Y,Wu Y,Liu L,He L,Gao J,Zhou L,Yu F,Yu S,Wang H

更新日期：2019-06-01 00:00:00

abstract：:To date, the calcareous tubes of serpulid marine worms have not been studied extensively in a biomineralization context. The structure and composition of the tube shell and adhesive cement of the marine tubeworm Hydroides dianthus were studied using a variety of characterization techniques, including powder XRD, FTIR,...

journal_title：Journal of structural biology

authors： Tanur AE,Gunari N,Sullan RM,Kavanagh CJ,Walker GC

更新日期：2010-02-01 00:00:00

abstract：:The analysis of structure factors in 3D cryo-EM Coulomb potential maps and their "enhancement" at the end of the reconstruction process is a well-established practice, normally referred to as sharpening. The aim is to increase contrast and, in this way, to help tracing the atomic model. The most common way to accompli...

journal_title：Journal of structural biology

authors： Vilas JL,Vargas J,Martinez M,Ramirez-Aportela E,Melero R,Jimenez-Moreno A,Garduño E,Conesa P,Marabini R,Maluenda D,Carazo JM,Sorzano COS

更新日期：2020-03-01 00:00:00

abstract：:Human coagulation factor VIIai that lacks the Gla domain (residues 1-44) has been prepared, purified, and crystallised. First, recombinant factor VII was activated to form factor VIIa, the active site was then inhibited with 1,5-dansyl-Glu-Gly-Arg-chloromethyl ketone, and finally the Gla domain was removed by chymotry...

journal_title：Journal of structural biology

authors： Johnson DJ,Nugent PG,Tuddenham EG,Harlos K,Kemball-Cook G

更新日期：1999-03-01 00:00:00

abstract：:Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) inactivates the aminoglycoside antibiotic hygromycin B (hygB) via phosphorylation. The crystal structure of the binary complex of APH(4)-Ia with hygB was recently reported. To characterize substrate recognition by the enzyme, we d...

journal_title：Journal of structural biology

authors： Iino D,Takakura Y,Fukano K,Sasaki Y,Hoshino T,Ohsawa K,Nakamura A,Yajima S

更新日期：2013-07-01 00:00:00

abstract：:Polynoid worm elytra emit light when mechanically or electrically stimulated. Specialized cells, the photocytes, contain light emitting machineries, the photosomes. Successive stimulations induce light intensity variations and show a coupling within and between photosomes. Here, we describe, using electron tomography ...

journal_title：Journal of structural biology

authors： Ouldali M,Maury V,Nicolas G,Lepault J

更新日期：2018-04-01 00:00:00

abstract：:The β-carbonic anhydrases (β-CAs) are widely distributed zinc-metalloenzymes that play essential roles in growth, survival, development and virulence in fungi. The majority of filamentous ascomycetes possess multiple β-CA isoforms among which major and minor forms have been characterized. We examined the catalytic beh...

journal_title：Journal of structural biology

authors： Kim S,Kim NJ,Hong S,Kim S,Sung J,Jin MS

更新日期：2019-10-01 00:00:00