Abstract:
:F-plasmid-mediated bacterial conjugation was studied with hfr (traDts) and tra I mutant Escherichia coli donor strains. This allowed us to observe a statistically significant number of conjugation-specific contacts by video and electron microscopy. Single mating events between E. coli were observed in real time by video-enhanced light microscopy. Conjugation in vivo takes place by initial contact formation via pili, followed by direct and transient wall-to-wall contact, during which DNA is transferred and disaggregated. Electron microscopic observations of the contact zone between donor and recipient bacteria were made by thin sectioning of mating pairs that were arranged in monolayers. We defined the conjugation-specific contact found in stabilized mating pairs as the conjugational junction. Within this junction no specific substructure such as plasma bridges by fusion could be detected during transfer of DNA.
journal_name
J Struct Bioljournal_title
Journal of structural biologyauthors
Dürrenberger MB,Villiger W,Bächi Tdoi
10.1016/1047-8477(91)90018-rsubject
Has Abstractpub_date
1991-10-01 00:00:00pages
146-56issue
2eissn
1047-8477issn
1095-8657pii
1047-8477(91)90018-Rjournal_volume
107pub_type
杂志文章abstract::Virus particle 24 (VP24) is the smallest protein of the Ebola and Marburg virus genomes. Recent experiments show that Ebola VP24 blocks binding of tyrosine-phosphorylated STAT-1 homodimer (PY-STAT1) to the NPI-1 subfamily of importin alpha, thereby preventing nuclear accumulation of this interferon-promoting transcrip...
journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
pub_type: 杂志文章
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/j.jsb.2012.10.002
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
pub_type: 杂志文章
doi:10.1016/1047-8477(91)90030-z
更新日期:1991-08-01 00:00:00
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journal_title:Journal of structural biology
pub_type: 杂志文章
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
pub_type: 杂志文章
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journal_title:Journal of structural biology
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journal_title:Journal of structural biology
pub_type: 杂志文章
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