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Silicon uptake and metabolism of the marine diatom Thalassiosira pseudonana: Solid-state 29Si NMR and fluorescence microscopic studies.

Abstract：

:Uptake and metabolism of silicon by diatoms are studied by the combined use of solid-state 29Si NMR spectroscopy and confocal laser fluorescence microscopy especially with respect to the presence and nature of an intracellular silicon-storage pool. Cells of the marine diatom Thalassiosira pseudonana were synchronized by silicon starvation and frozen without any freeze-drying or chemical treatment in order to analyze integer and unmodified diatoms. The frozen samples were investigated by solid-state 29Si NMR spectroscopy to identify potential silica precursors. The developmental state of the cell culture and the formation of new siliceous girdle bands and valves were monitored by laser fluorescence microscopic studies. A comparison of fluorescence microscopic and NMR data allows the assignment of NMR spectra to the various developmental stages of the dividing diatom cells. A detailed analysis of solid-state 29Si NMR spectra suggests that the silicon-storage pool-if present-consists of four-coordinated, condensed silicon; possibly a silica sol.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Gröger C,Sumper M,Brunner E

doi

10.1016/j.jsb.2007.09.010

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

55-63

issue

1

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(07)00228-6

journal_volume

161

pub_type

杂志文章

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