当前位置： SCI文献检索 > JOURNAL OF STRUCTURAL BIOLOGY期刊下所有文献 > Automated image acquisition and processing using a new generation of 4K x 4K CCD cameras for cryo electron microscopic studies of macromolecular assemblies.

Automated image acquisition and processing using a new generation of 4K x 4K CCD cameras for cryo electron microscopic studies of macromolecular assemblies.

Abstract：

:We have previously reported the development of AutoEM, a software package for semi-automated acquisition of data from a transmission electron microscope. In continuing efforts to improve the speed of structure determination of macromolecular assemblies by electron microscopy, we report here on the performance of a new generation of 4 K CCD cameras for use in cryo electron microscopic applications. We demonstrate that at 120 kV, and at a nominal magnification of 67000 x, power spectra and signal-to-noise ratios for the new 4 K CCD camera are comparable to values obtained for film images scanned using a Zeiss scanner to resolutions as high as approximately 1/6.5A(-1). The specimen area imaged for each exposure on the 4 K CCD is about one-third of the area that can be recorded with a similar exposure on film. The CCD camera also serves the purpose of recording images at low magnification from the center of the hole to measure the thickness of vitrified ice in the hole. The performance of the camera is satisfactory under the low-dose conditions used in cryo electron microscopy, as demonstrated here by the determination of a three-dimensional map at 15 A for the catalytic core of the 1.8 MDa Bacillus stearothermophilus icosahedral pyruvate dehydrogenase complex, and its comparison with the previously reported atomic model for this complex obtained by X-ray crystallography.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Zhang P,Borgnia MJ,Mooney P,Shi D,Pan M,O'Herron P,Mao A,Brogan D,Milne JL,Subramaniam S

doi

10.1016/s1047-8477(03)00124-2

keywords:

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

135-44

issue

2

eissn

1047-8477

issn

1095-8657

pii

S1047847703001242

journal_volume

143

pub_type

杂志文章

相关文献

JOURNAL OF STRUCTURAL BIOLOGY文献大全
  • A new HIV-1 Rev structure optimizes interaction with target RNA (RRE) for nuclear export.

    abstract：:HIV-1 Rev mediates the nuclear export of unspliced and partially-spliced viral transcripts for the production of progeny genomes and structural proteins. In this process, four (or more) copies of Rev assemble onto a highly-structured 351-nt region in such viral transcripts, the Rev response element (RRE). How this occ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.03.011

    authors： Watts NR,Eren E,Zhuang X,Wang YX,Steven AC,Wingfield PT

    更新日期：2018-08-01 00:00:00

  • The bacteriocin AS-48 requires dimer dissociation followed by hydrophobic interactions with the membrane for antibacterial activity.

    abstract：:The molecular mechanism underlining the antibacterial activity of the bacteriocin AS-48 is not known, and two different and opposite alternatives have been proposed. Available data suggested that the interaction of positively charged amino acids of AS-48 with the membrane would produce membrane destabilization and dis...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2015.03.006

    authors： Cebrián R,Martínez-Bueno M,Valdivia E,Albert A,Maqueda M,Sánchez-Barrena MJ

    更新日期：2015-05-01 00:00:00

  • The structural characteristics of mononuclear-macrophage membrane observed by atomic force microscopy.

    abstract：:Mononuclear macrophages are important immune cells in the organisms. The complicated membrane structure underlying the diverse functions of mononuclear-macrophage has been largely unresolved. As a representative of monocyte-derived macrophages, the membrane structure of PMA differentiated THP-1 cells was comprehensive...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2019.04.002

    authors： Tian Y,Wu Y,Liu L,He L,Gao J,Zhou L,Yu F,Yu S,Wang H

    更新日期：2019-06-01 00:00:00

  • Influence of microstructure on deformation anisotropy of mineralized cuticle from the lobster Homarus americanus.

    abstract：:The exoskeleton of the American lobster Homarus americanus is a hierarchically organized nano-composite material consisting of organic chitin-protein fibers associated with inorganic calcium carbonate. The presence of a well-developed and periodically arranged pore canal system leads to a honeycomb-like structure. The...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2007.09.022

    authors： Sachs C,Fabritius H,Raabe D

    更新日期：2008-02-01 00:00:00

  • A 9 angstroms single particle reconstruction from CCD captured images on a 200 kV electron cryomicroscope.

    abstract：:Sub-nanometer resolution structure determination is becoming a common practice in electron cryomicroscopy of macromolecular assemblies. The data for these studies have until now been collected on photographic film. Using cytoplasmic polyhedrosis virus (CPV), a previously determined structure, as a test specimen, we sh...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2004.02.004

    authors： Booth CR,Jiang W,Baker ML,Zhou ZH,Ludtke SJ,Chiu W

    更新日期：2004-08-01 00:00:00

  • High-resolution structures of annexin A5 in a two-dimensional array.

    abstract：:Annexins are soluble cytosolic proteins that bind to cell membranes. Annexin A5 self-assembles into a two-dimensional (2D) array and prevents cell rupture by attaching to damaged membranes. However, this process is not fully understood at the molecular level. In this study, we determined the crystal structures of anne...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2019.10.003

    authors： Hong S,Na S,Kim OH,Jeong S,Oh BC,Ha NC

    更新日期：2020-01-01 00:00:00

  • Structural proteins of three viruses in the Potyviridae adhere only to their homologous cylindrical inclusions in mixed infections.

    abstract：:Immunoelectron microscopy of serial sections of leaf cells of wheat doubly infected by wheat streak mosaic virus and Agropyron mosaic virus or wheat spindle streak mosaic virus showed that structural proteins or virions associated only with homologous cylindrical inclusions (CI) or with self in aggregates. CI protein ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1993.1021

    authors： Langenberg WG

    更新日期：1993-05-01 00:00:00

  • Conical Fourier shell correlation applied to electron tomograms.

    abstract：:The resolution of electron tomograms is anisotropic due to geometrical constraints during data collection, such as the limited tilt range and single axis tilt series acquisition. Acquisition of dual axis tilt series can decrease these effects. However, in cryo-electron tomography, to limit the electron radiation damag...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2015.03.010

    authors： Diebolder CA,Faas FG,Koster AJ,Koning RI

    更新日期：2015-05-01 00:00:00

  • Crystallization of sparingly soluble stress-related proteins from cyanobacteria by controlled urea solublization.

    abstract：:The phycobilisome photosynthetic antenna complex, found in cyanobacteria and red-algae, interacts with proteins expressed specifically to deal with different forms of physiological stress. Under conditions of nutrient starvation, the NblA protein is required for the process that leads to phycobilisome degradation and ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2006.10.021

    authors： Dines M,Sendersky E,Schwarz R,Adir N

    更新日期：2007-04-01 00:00:00

  • Looking into live cells with in-cell NMR spectroscopy.

    abstract：:In-cell NMR spectroscopy has gained recent popularity since it provides means to analyze the conformational and functional properties of proteins inside living cells and at atomic resolution. High-resolution in-cell NMR spectroscopy was originally established in bacterial cells and based on a rationale that relies on ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jsb.2007.04.001

    authors： Selenko P,Wagner G

    更新日期：2007-05-01 00:00:00

  • Design of net-charged abc-type collagen heterotrimers.

    abstract：:Net-negatively-charged heterospecific A:B:C collagen peptide heterotrimers were designed using an automated computational approach. The design algorithm considers both target stability and the energy gap between the target states and misfolded competing states. Structural characterization indicates the net-negative ch...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2013.04.006

    authors： Parmar AS,Zahid S,Belure SV,Young R,Hasan N,Nanda V

    更新日期：2014-02-01 00:00:00

  • A novel small molecule displays two different binding modes during inhibiting H1N1 influenza A virus neuraminidases.

    abstract：:Neuraminidase (NA) inhibitors can suppress NA activity to block the release of progeny virions and are effective against influenza viruses. As potential anti-flu drugs with unique functions, NA inhibitors are greatly concerned by the worldwide scientists. It has been reported recently that one of the novel quindoline ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2017.12.014

    authors： Guan S,Xu Y,Qiao Y,Kuai Z,Qian M,Jiang X,Wang S,Zhang H,Kong W,Shan Y

    更新日期：2018-05-01 00:00:00

  • Automatic alignment of transmission electron microscope tilt series without fiducial markers.

    abstract：:Accurate image alignment is needed for computing three-dimensional reconstructions from transmission electron microscope tilt series. So far, the best results have been obtained by using colloidal gold beads as fiducial markers. If their use has not been possible for some reason, the only option has been the automatic...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.2001.4443

    authors： Brandt S,Heikkonen J,Engelhardt P

    更新日期：2001-12-01 00:00:00

  • Structural characteristics of the beta-sheet-like human and rat islet amyloid polypeptides as determined by scanning tunneling microscopy.

    abstract：:We demonstrate in this work that scanning tunneling microscopy (STM) provides a useful approach to obtaining structural information about human islet amyloid polypeptide (hIAPP) and rat islet amyloid polypeptide (rIAPP) assembly on highly oriented pyrolytic graphite (HOPG) with sub-molecular resolution. The observed h...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.05.009

    authors： Mao X,Ma X,Liu L,Niu L,Yang Y,Wang C

    更新日期：2009-09-01 00:00:00

  • Variation in human hair ultrastructure among three biogeographic populations.

    abstract：:Human scalp hairs are often examined microscopically to study the variation and diversity among a range of visible morphological traits. In this study, we focused on the ultrastructure of human scalp hair within its keratinized matrix, emphasizing, the density and distribution of melanosomes, variation in cuticle thic...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2018.11.008

    authors： Koch SL,Shriver MD,Jablonski NG

    更新日期：2019-01-01 00:00:00

  • Structure of cardiac gap junction intercellular channels.

    abstract：:Gap junction proteins, termed connexins, constitute a multigene family of polytopic membrane channel proteins that have four hydrophobic transmembrane domains with the N- and C-termini located on the cytoplasmic membrane face. The principal gap junction protein in the heart, alpha 1 connexin (also designated Cx43), me...

    journal_title：Journal of structural biology

    pub_type： 杂志文章,评审

    doi：10.1006/jsbi.1998.3972

    authors： Yeager M

    更新日期：1998-01-01 00:00:00

  • The crystallographic structure of Panicum Mosaic Virus (PMV).

    abstract：:The structure of Panicum Mosaic Virus (PMV) was determined by X-ray diffraction analysis to 2.9Å resolution. The crystals were of pseudo symmetry F23; the true crystallographic unit cell was of space group P2(1) with a=411.7Å, b=403.9Å and c=412.5Å, with β=89.7°. The asymmetric unit was two entire T=3 virus particles,...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2012.10.012

    authors： Makino DL,Larson SB,McPherson A

    更新日期：2013-01-01 00:00:00

  • Utility of Butvar support film and methylamine tungstate stain in three-dimensional electron microscopy: agreement between stain and frozen-hydrated reconstructions.

    abstract：:A random conical tilt reconstruction of negatively stained Saccharomyces cerevisiae fatty acid synthase was used as a model to compute a three-dimensional reconstruction from untilted stain specimens of the molecules in multiple orientations using a three-dimensional projection alignment method. The resulting structur...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1997.3911

    authors： Kolodziej SJ,Penczek PA,Stoops JK

    更新日期：1997-11-01 00:00:00

  • Synchrotron microComputed Tomography of the mature bovine dentinoenamel junction.

    abstract：:The mature dentinoenamel junction (DEJ) is viewed by some investigators and the current authors, not as a fossilized, sharp transition between enamel and dentin, but as a relatively broad structural transition zone including the mantle dentin and the inner aprismatic enamel. In this study, the DEJ structure in bovine ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2007.10.006

    authors： Stock SR,Vieira AE,Delbem AC,Cannon ML,Xiao X,Carlo FD

    更新日期：2008-02-01 00:00:00

  • Analysis of immunolabeled cells by atomic force microscopy, optical microscopy, and flow cytometry.

    abstract：:In this study we investigated the applicability of the (silver-enhanced) immunogold labeling method for atomic force microscopy. Human lymphocytes were labeled with anti-CD3 conjugated to fluorescein isothiocyanate and a secondary antibody (goat anti-mouse) linked with 1- or 30-nm colloidal gold particles. Silver enha...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1994.1004

    authors： Neagu C,van der Werf KO,Putman CA,Kraan YM,de Grooth BG,van Hulst NF,Greve J

    更新日期：1994-01-01 00:00:00

  • Synthesis and antimicrobial activity of truncated fragments and analogs of citropin 1.1: The solution structure of the SDS micelle-bound citropin-like peptides.

    abstract：:Citropin 1.1 is a basic, highly hydrophobic, 16-amino acid peptide (GLFDVIKKVASVIGGL-NH(2)), displaying wide-spectrum antimicrobial activities. In this paper we describe the synthesis and antimicrobial properties of citropin 1.1 and its 18 analogs constituting mostly truncated fragments of citropin 1.1. Moreover, we e...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.07.012

    authors： Sikorska E,Greber K,Rodziewicz-Motowidło S,Szultka L,Lukasiak J,Kamysz W

    更新日期：2009-11-01 00:00:00

  • 3D imaging of diatoms with ion-abrasion scanning electron microscopy.

    abstract：:Ion-abrasion scanning electron microscopy (IASEM) takes advantage of focused ion beams to abrade thin sections from the surface of bulk specimens, coupled with SEM to image the surface of each section, enabling 3D reconstructions of subcellular architecture at approximately 30nm resolution. Here, we report the first a...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.02.014

    authors： Hildebrand M,Kim S,Shi D,Scott K,Subramaniam S

    更新日期：2009-06-01 00:00:00

  • The three-dimensional structure of trypsin-treated Staphylococcus aureus alpha-toxin.

    abstract：:Trypsin treatment of staphylococcal alpha-toxin cleaves the molecule into two roughly equally sized parts, which results in inactivation of the toxin. Tetragonal arrays of oligomers, closely resembling the native ones, can however be formed on lipid layers. From tilted views of negatively stained crystals a 3D structu...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/1047-8477(92)90024-5

    authors： Olofsson A,Kavéus U,Thelestam M,Hebert H

    更新日期：1992-05-01 00:00:00

  • Three-dimensional reconstruction of collagen-proteoglycan interactions in the mouse corneal stroma by electron tomography.

    abstract：:Corneal transparency is fundamental to the visual system, and is directly related to the ordered collagen fibril architecture that the cornea maintains. Proteoglycans, through their protein core and highly anionic glycosaminoglycan side chains, are thought to regulate the collagen organisation in the corneal stroma. T...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2010.01.019

    authors： Parfitt GJ,Pinali C,Young RD,Quantock AJ,Knupp C

    更新日期：2010-05-01 00:00:00

  • Automated molecular microscopy: the new Leginon system.

    abstract：:We report here on the current state of our efforts in automated molecular microscopy. Our primary automated data acquisition software system, Leginon, has been completely redesigned over the past two years. The new distributed system has been developed using the Python programming language and is compatible with both ...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2005.03.010

    authors： Suloway C,Pulokas J,Fellmann D,Cheng A,Guerra F,Quispe J,Stagg S,Potter CS,Carragher B

    更新日期：2005-07-01 00:00:00

  • Preservation of 2-D crystals of tubulin for electron crystallography.

    abstract：:Zinc-induced sheets of tubulin are two-dimensional crystalline polymers that constitute an ideal sample for high resolution studies of tubulin by electron crystallography. We show that these 2-dimensional tubulin crystals can be stabilized by taxol against low-temperature depolymerization and degradation with time, ea...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1006/jsbi.1995.1044

    authors： Nogales E,Wolf SG,Zhang SX,Downing KH

    更新日期：1995-09-01 00:00:00

  • Large nuclear RNP particles--the nuclear pre-mRNA processing machine.

    abstract：:Processing of nuclear pre-mRNA is an important step in the regulation of gene expression and involves 5(')- and 3(')-end processing, splicing, and editing. Mammalian nuclear pre-mRNAs are assembled in large ribonucleoprotein (lnRNP) complexes, in which the entire population of nuclear pre-mRNA is individually packaged...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/s1047-8477(02)00541-5

    authors： Raitskin O,Angenitzki M,Sperling J,Sperling R

    更新日期：2002-10-01 00:00:00

  • Topological mapping of 13 epitopes on a subunit of Androctonus australis hemocyanin.

    abstract：:A topological localization of epitopes on the surface of the Aa6 subunit of Androctonus australis hemocyanin has been carried out. First, immunocomplex strings composed of native hemocyanin and monoclonal antibodies were examined in the electron microscope and submitted to an image processing by correspondence analysi...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/1047-8477(90)90087-s

    authors： Lamy J,Billiald P,Taveau JC,Boisset N,Motta G,Lamy J

    更新日期：1990-03-01 00:00:00

  • Detecting molecular interactions that stabilize, activate and guide ligand-binding of the sodium/proton antiporter MjNhaP1 from Methanococcus jannaschii.

    abstract：:Integral membrane proteins are involved in virtually every cellular process. Precisely regulating these machineries would allow controlling many human and vertebrate diseases. Embedded into cellular membranes, membrane proteins establish molecular interactions that sensitively react to environmental changes and to mol...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2007.02.010

    authors： Kedrov A,Wegmann S,Smits SH,Goswami P,Baumann H,Muller DJ

    更新日期：2007-08-01 00:00:00

  • A different conformation for linker L12 in IF molecules in the molecular and filamentous forms: an hypothesis.

    abstract：:The rod domain of IF molecules has been characterized as four alpha-helical coiled-coil segments (1A, 1B, 2A and 2B), three linkers (L1, L12 and L2) and a stutter at the centre of segment 2B. Two of these breaks in coiled-coil continuity (L2 and stutter) have been modelled on the basis of structural data obtained from...

    journal_title：Journal of structural biology

    pub_type： 杂志文章

    doi：10.1016/j.jsb.2009.12.006

    authors： Parry DA,Smith TA

    更新日期：2010-05-01 00:00:00