Crystallization and preliminary X-ray diffraction analysis of UDP-N-acetylglucosamine enolpyruvyltransferase of Enterobacter cloacae.

Abstract:

:Single crystals of UDP-N-acetylglucosamine enolpyruvyltransferase of Enterobacter cloacae have been grown by vapor diffusion using phosphate buffer as the precipitant. The crystals belong to the monoclinic space group C2 with a = 86.9 A, b = 155.9 A, c = 83.8 A, beta = 91.6 degrees. Assuming two monomers per asymmetric unit, the solvent content of these crystals is 63%. Flash-frozen crystals diffract to beyond 2 A resolution.

journal_name

J Struct Biol

authors

Sack S,Dauter Z,Wanke C,Amrhein N,Mandelkow E,Schönbrunn E

doi

10.1006/jsbi.1996.0071

subject

Has Abstract

pub_date

1996-07-01 00:00:00

pages

73-6

issue

1

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(96)90071-4

journal_volume

117

pub_type

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