解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Multiple lines of evidence suggest that functionally intact cerebello-hippocampal interactions are required for appropriate spatial processing. However, how the cerebellum anatomically and physiologically engages with the hippocampus to sustain such communication remains unknown. Using rabies virus as a retrograde tra...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.41896
更新日期:2019-06-17 00:00:00
abstract::The mammalian auditory sensory epithelium has one of the most stereotyped cellular patterns known in vertebrates. Mechano-sensory hair cells are arranged in precise rows, with one row of inner and three rows of outer hair cells spanning the length of the spiral-shaped sensory epithelium. Aiding such precise cellular p...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47613
更新日期:2019-06-12 00:00:00
abstract::We present the development and in vivo application of a feedback-based tracking microscope to follow individual mitochondria in sensory neurons of zebrafish larvae with nanometer precision and millisecond temporal resolution. By combining various technical improvements, we tracked individual mitochondria with unpreced...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46059
更新日期:2019-06-10 00:00:00
abstract::GPIHBP1, a GPI-anchored protein of capillary endothelial cells, binds lipoprotein lipase (LPL) within the subendothelial spaces and shuttles it to the capillary lumen. GPIHBP1-bound LPL is essential for the margination of triglyceride-rich lipoproteins (TRLs) along capillaries, allowing the lipolytic processing of TRL...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47178
更新日期:2019-06-06 00:00:00
abstract::Transposable elements (TEs), the movement of which can damage the genome, are epigenetically silenced in eukaryotes. Intriguingly, TEs are activated in the sperm companion cell - vegetative cell (VC) - of the flowering plant Arabidopsis thaliana. However, the extent and mechanism of this activation are unknown. Here w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.42530
更新日期:2019-05-28 00:00:00
abstract::Allosteric HIV-1 integrase (IN) inhibitors (ALLINIs) are a promising new class of antiretroviral agents that disrupt proper viral maturation by inducing hyper-multimerization of IN. Here we show that lead pyridine-based ALLINI KF116 exhibits striking selectivity for IN tetramers versus lower order protein oligomers. I...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46344
更新日期:2019-05-23 00:00:00
abstract::Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.43079
更新日期:2019-05-21 00:00:00
abstract::Oncogenic human papillomaviruses (HPV) are small DNA viruses that infect keratinocytes. After HPV binding to cell surface receptors, a cascade of molecular interactions mediates the infectious cellular internalization of virus particles. Aside from the virus itself, important molecular players involved in virus entry ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44345
更新日期:2019-05-20 00:00:00
abstract::Understanding the molecular mechanism of glaucoma and development of neuroprotectants is significantly hindered by the lack of a reliable animal model that accurately recapitulates human glaucoma. Here, we sought to develop a mouse model for the secondary glaucoma that is often observed in humans after silicone oil (S...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45881
更新日期:2019-05-15 00:00:00
abstract::Two-dimensional (2D) human skeletal muscle fiber cultures are ill-equipped to support the contractile properties of maturing muscle fibers. This limits their application to the study of adult human neuromuscular junction (NMJ) development, a process requiring maturation of muscle fibers in the presence of motor neuron...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44530
更新日期:2019-05-14 00:00:00
abstract::To effectively interact with their environment, humans must often select actions from multiple incompatible options. Existing theories propose that during motoric response-conflict, inappropriate motor activity is actively (and perhaps non-selectively) suppressed by an inhibitory fronto-basal ganglia mechanism. We her...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.42959
更新日期:2019-05-07 00:00:00
abstract::Protein concentration gradients pattern developing organisms and single cells. In Schizosaccharomyces pombe rod-shaped cells, Pom1 kinase forms gradients with maxima at cell poles. Pom1 controls the timing of mitotic entry by inhibiting Cdr2, which forms stable membrane-associated nodes at mid-cell. Pom1 gradients rel...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45983
更新日期:2019-05-03 00:00:00
abstract::We are writing to comment on the recent study by Olesen et al., 2018 on antibiotic use and antibiotic resistance. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.46561
更新日期:2019-05-03 00:00:00
abstract::Ten countries have reported pfhrp2/pfhrp3 gene deletions since the first observation of pfhrp2-deleted parasites in 2012. In a previous study (Watson et al., 2017), we characterised the drivers selecting for pfhrp2/3 deletions and mapped the regions in Africa with the greatest selection pressure. In February 2018, the...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40339
更新日期:2019-05-02 00:00:00
abstract::The expansion of bacterial swarms and the spreading of biofilms can be described by a unified biophysical theory that involves both active and passive processes. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47019
更新日期:2019-04-30 00:00:00
abstract::When an individual makes a judgement about the actions of another individual, taking the latter's viewpoint into consideration enhances cooperation in society at large. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47036
更新日期:2019-04-29 00:00:00
abstract::Iron and heme play central roles in the production of red blood cells, but the underlying mechanisms remain incompletely understood. Heme-regulated eIF2α kinase (HRI) controls translation by phosphorylating eIF2α. Here, we investigate the global impact of iron, heme, and HRI on protein translation in vivo in murine pr...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46976
更新日期:2019-04-29 00:00:00
abstract::Protein aggregation appears to originate from partially unfolded conformations that are sampled through stochastic fluctuations of the native protein. It has been a challenge to characterize these fluctuations, under native like conditions. Here, the conformational dynamics of the full-length (23-231) mouse prion prot...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44766
更新日期:2019-04-26 00:00:00
abstract::In complex biological systems, simple individual-level behavioral rules can give rise to emergent group-level behavior. While collective behavior has been well studied in cells and larger organisms, the mesoscopic scale is less understood, as it is unclear which sensory inputs and physical processes matter a priori. H...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.43318
更新日期:2019-04-25 00:00:00
abstract::The growth of preprints in the life sciences has been reported widely and is driving policy changes for journals and funders, but little quantitative information has been published about preprint usage. Here, we report how we collected and analyzed data on all 37,648 preprints uploaded to bioRxiv.org, the largest biol...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45133
更新日期:2019-04-24 00:00:00
abstract::Unfolded protein responses (UPRs) safeguard cellular function during proteotoxic stress and aging. In a previous paper (Lehrbach and Ruvkun, 2016) we showed that the ER-associated SKN-1A/Nrf1 transcription factor activates proteasome subunit expression in response to proteasome dysfunction, but it was not established ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44425
更新日期:2019-04-11 00:00:00
abstract::We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown. Here we show E. faecium has unique peptidoglycan comp...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45343
更新日期:2019-04-10 00:00:00
abstract::Two recently discovered transcription factors stop cells from dividing when plants face extreme heat and DNA damage. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.46781
更新日期:2019-04-09 00:00:00
abstract::We recently implemented a bioinformatics pipeline that can uncover novel, but rare, riboswitch candidates as well as other noncoding RNA structures in bacteria. A prominent candidate revealed by our initial search efforts was called the 'thiS motif' because of its frequent association with a gene coding for the ThiS p...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45210
更新日期:2019-04-05 00:00:00
abstract::An individual may need to take different actions to the same stimulus in different situations to achieve a given goal. The selection of the appropriate action hinges on the previously learned associations between stimuli, actions, and outcomes in the situations. Here, using a go/no-go paradigm and a symmetrical reward...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.43281
更新日期:2019-04-04 00:00:00
abstract::The integrity of the nuclear membranes coupled to the selective barrier of nuclear pore complexes (NPCs) are essential for the segregation of nucleoplasm and cytoplasm. Mechanical membrane disruption or perturbation to NPC assembly triggers an ESCRT-dependent surveillance system that seals nuclear pores: how these por...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45284
更新日期:2019-04-03 00:00:00
abstract::CRISPR-Cas systems provide sequence-specific immunity against phages and mobile genetic elements using CRISPR-associated nucleases guided by short CRISPR RNAs (crRNAs). Type III systems exhibit a robust immune response that can lead to the extinction of a phage population, a feat coordinated by a multi-subunit effecto...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45393
更新日期:2019-04-03 00:00:00
abstract::Mitogen-activated protein kinases (MAPK) such as p38 and the c-Jun N-terminal kinases (JNKs) are activated during the cellular response to stress signals. Their activity is regulated by the MAPK-phosphatase 1 (DUSP1), a key component of the anti-inflammatory response. Stress kinases are well-described elements of the ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.39159
更新日期:2019-04-02 00:00:00
abstract::Cell-cell communication in multicellular organisms depends on the dynamic and reversible phosphorylation of protein tyrosine residues. The receptor-linked protein tyrosine phosphatases (RPTPs) receive cues from the extracellular environment and are well placed to influence cell signaling. However, the direct events do...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44597
更新日期:2019-03-29 00:00:00
abstract::Class IV ddaC neurons specifically prune larval dendrites without affecting axons during Drosophila metamorphosis. ddaCs distribute the minus ends of microtubules (MTs) to dendrites but the plus ends to axons. However, a requirement of MT minus-end-binding proteins in dendrite-specific pruning remains completely unkno...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.39964
更新日期:2019-03-28 00:00:00
abstract::Combining clonal analysis with a computational agent based model, we investigate how tissue-specific stem cells for neural retina (NR) and retinal pigmented epithelium (RPE) of the teleost medaka (Oryzias latipes) coordinate their growth rates. NR cell division timing is less variable, consistent with an upstream role...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.42646
更新日期:2019-03-26 00:00:00
abstract::Episodic memories reflect a bound representation of multimodal features that can be reinstated with varying precision. Yet little is known about how brain networks involved in memory, including the hippocampus and posterior-medial (PM) and anterior-temporal (AT) systems, interact to support the quality and content of ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45591
更新日期:2019-03-22 00:00:00
abstract::Humans can run without falling down, usually despite uneven terrain or occasional pushes. Even without such external perturbations, intrinsic sources like sensorimotor noise perturb the running motion incessantly, making each step variable. Here, using simple and generalizable models, we show that even such small step...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.38371
更新日期:2019-03-19 00:00:00
abstract::Appropriate generalization of learned responses to new situations is vital for adaptive behavior. We provide a circuit-level account of generalization in the electrosensory lobe (ELL) of weakly electric mormyrid fish. Much is already known in this system about a form of learning in which motor corollary discharge sign...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44032
更新日期:2019-03-14 00:00:00
abstract::Linking interindividual differences in psychological phenotype to variations in brain structure is an old dream for psychology and a crucial question for cognitive neurosciences. Yet, replicability of the previously-reported 'structural brain behavior' (SBB)-associations has been questioned, recently. Here, we conduct...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.43464
更新日期:2019-03-13 00:00:00
abstract::A founding paradigm in virology is that the spatial unit of the viral replication cycle is an individual cell. Multipartite viruses have a segmented genome where each segment is encapsidated separately. In this situation the viral genome is not recapitulated in a single virus particle but in the viral population. How ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.43599
更新日期:2019-03-12 00:00:00
abstract::We report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface. The nature of this interface coupled with quantitative analysis of deletion mutants of H2A-H2B suggests that the N...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.43630
更新日期:2019-03-11 00:00:00
abstract::Calcium is a critical second messenger in neurons that contributes to learning and memory, but how the coordination of action potentials of neuronal ensembles with the hippocampal local field potential (LFP) is reflected in dynamic calcium activity remains unclear. Here, we recorded hippocampal calcium activity with e...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.39777
更新日期:2019-03-07 00:00:00
abstract::India comprises much of the persisting global childhood measles mortality. India implemented a mass second-dose measles immunization campaign in 2010. We used interrupted time series and multilevel regression to quantify the campaign's impact on measles mortality using the nationally representative Million Death Study...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.43290
更新日期:2019-03-05 00:00:00
abstract::The biological players involved in angiogenesis are only partially defined. Here, we report that endothelial cells (ECs) express a novel isoform of the cell-surface adhesion molecule L1CAM, termed L1-ΔTM. The splicing factor NOVA2, which binds directly to L1CAM pre-mRNA, is necessary and sufficient for the skipping of...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44305
更新日期:2019-03-04 00:00:00