Abstract:
:Calcium is a critical second messenger in neurons that contributes to learning and memory, but how the coordination of action potentials of neuronal ensembles with the hippocampal local field potential (LFP) is reflected in dynamic calcium activity remains unclear. Here, we recorded hippocampal calcium activity with endoscopic imaging of the genetically encoded fluorophore GCaMP6 with concomitant LFP in freely behaving mice. Dynamic calcium activity was greater in exploratory behavior and REM sleep than in quiet wakefulness and slow wave sleep, behavioral states that differ with respect to theta and septal cholinergic activity, and modulated at sharp wave ripples (SWRs). Chemogenetic activation of septal cholinergic neurons expressing the excitatory hM3Dq DREADD increased calcium activity and reduced SWRs. Furthermore, inhibition of muscarinic acetylcholine receptors (mAChRs) reduced calcium activity while increasing SWRs. These results demonstrate that hippocampal dynamic calcium activity depends on behavioral and theta state as well as endogenous mAChR activation.
journal_name
Elifejournal_title
eLifeauthors
Zhou H,Neville KR,Goldstein N,Kabu S,Kausar N,Ye R,Nguyen TT,Gelwan N,Hyman BT,Gomperts SNdoi
10.7554/eLife.39777subject
Has Abstractpub_date
2019-03-07 00:00:00issn
2050-084Xpii
39777journal_volume
8pub_type
杂志文章相关文献
eLife文献大全abstract::Using knowledge of the structure of the world to infer value is at the heart of model-based reasoning and relies on a circuit that includes the orbitofrontal cortex (OFC). Some accounts link this to the representation of biological significance or value by neurons in OFC, while other models focus on the representation...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30373
更新日期:2018-03-07 00:00:00
abstract::The fitness effects of synonymous mutations, nucleotide changes that do not alter the encoded amino acid, have often been assumed to be neutral, but a growing body of evidence suggests otherwise. We used site-directed mutagenesis coupled with direct measures of competitive fitness to estimate the distribution of fitne...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45952
更新日期:2019-07-19 00:00:00
abstract::As part of the Reproducibility Project: Cancer Biology, we published a Registered Report (Blum et al., 2015), that described how we intended to replicate selected experiments from the paper 'Transcriptional amplification in tumor cells with elevated c-Myc' (Lin et al., 2012). Here we report the results. We found overe...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30274
更新日期:2018-01-09 00:00:00
abstract::SynGAP is a postsynaptic density (PSD) protein that binds to PDZ domains of the scaffold protein PSD-95. We previously reported that heterozygous deletion of Syngap1 in mice is correlated with increased steady-state levels of other key PSD proteins that bind PSD-95, although the level of PSD-95 remains constant (Walku...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52656
更新日期:2020-01-15 00:00:00
abstract::Mycobacterium tuberculosis (Mtb) expresses a broad-spectrum β-lactamase (BlaC) that mediates resistance to one of the highly effective antibacterials, β-lactams. Nonetheless, β-lactams showed mycobactericidal activity in combination with β-lactamase inhibitor, clavulanate (Clav). However, the mechanistic aspects of ho...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25624
更新日期:2017-05-26 00:00:00
abstract::The >800 human G protein-coupled receptors (GPCRs) are responsible for transducing diverse chemical stimuli to alter cell state- and are the largest class of drug targets. Their myriad structural conformations and various modes of signaling make it challenging to understand their structure and function. Here, we devel...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54895
更新日期:2020-10-21 00:00:00
abstract::Microtubules control different aspects of cell polarization. In cells with a radial microtubule system, a pivotal role in setting up asymmetry is attributed to the relative positioning of the centrosome and the nucleus. Here, we show that centrosome loss had no effect on the ability of endothelial cells to polarize an...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.33864
更新日期:2018-03-16 00:00:00
abstract::Gap junctions are widely distributed in the brains across species and play essential roles in neural information processing. However, the role of gap junctions in insect cognition remains poorly understood. Using a flight simulator paradigm and genetic tools, we found that gap junctions are present in Drosophila Kenyo...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.13238
更新日期:2016-05-24 00:00:00
abstract::Genetic analyses of 15 species of yeast have shed new light on the divergence of gene regulation during evolution, with significant changes occurring after an event in which a whole genome was duplicated. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.00933
更新日期:2013-06-18 00:00:00
abstract::Secretory (S) Immunoglobulin (Ig) A is the predominant mucosal antibody, which binds pathogens and commensal microbes. SIgA is a polymeric antibody, typically containing two copies of IgA that assemble with one joining-chain (JC) to form dimeric (d) IgA that is bound by the polymeric Ig-receptor ectodomain, called sec...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56098
更新日期:2020-10-27 00:00:00
abstract::Homologous recombination is widespread and catalyzes evolution. Nonetheless, its existence in animal mitochondrial DNA is questioned. We designed selections for recombination between co-resident mitochondrial genomes in various heteroplasmic Drosophila lines. In four experimental settings, recombinant genomes became t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07247
更新日期:2015-08-03 00:00:00
abstract::In response to cell death signals, an active apoptosome is assembled from Apaf-1 and procaspase-9 (pc-9). Here we report a near atomic structure of the active human apoptosome determined by cryo-electron microscopy. The resulting model gives insights into cytochrome c binding, nucleotide exchange and conformational ch...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17755
更新日期:2016-10-04 00:00:00
abstract::During translation elongation, the ribosome ratchets along its mRNA template, incorporating each new amino acid and translocating from one codon to the next. The elongation cycle requires dramatic structural rearrangements of the ribosome. We show here that deep sequencing of ribosome-protected mRNA fragments reveals ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01257
更新日期:2014-05-09 00:00:00
abstract::PKMζ is a persistently active PKC isoform proposed to maintain late-LTP and long-term memory. But late-LTP and memory are maintained without PKMζ in PKMζ-null mice. Two hypotheses can account for these findings. First, PKMζ is unimportant for LTP or memory. Second, PKMζ is essential for late-LTP and long-term memory i...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14846
更新日期:2016-05-17 00:00:00
abstract::The organization and biophysical properties of the cytosol implicitly govern molecular interactions within cells. However, little is known about mechanisms by which cells regulate cytosolic properties and intracellular diffusion rates. Here, we demonstrate that the intracellular environment of budding yeast undertakes...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.09376
更新日期:2016-03-22 00:00:00
abstract::Manipulating gene function cell type-specifically is a common experimental goal in Drosophila research and has been central to studies of neural development, circuit computation, and behavior. However, current cell type-specific gene disruption techniques in flies often reduce gene activity incompletely or rely on cel...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22279
更新日期:2017-02-17 00:00:00
abstract::Cell fate decisions are critical for life, yet little is known about how their reliability is achieved when signals are noisy and fluctuating with time. In this study, we show that in budding yeast, the decision of cell cycle commitment (Start) is determined by the time integration of its triggering signal Cln3. We fu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03977
更新日期:2015-01-14 00:00:00
abstract::The restoration of homeostasis after tissue damage relies on proper spatial-temporal control of damage-induced apoptosis and compensatory proliferation. In Drosophila imaginal discs these processes are coordinated by the stress response pathway JNK. We demonstrate that JNK signaling induces a dose-dependent extension ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.41036
更新日期:2019-02-08 00:00:00
abstract::The human insular cortex is a heterogeneous brain structure which plays an integrative role in guiding behavior. The cytoarchitectonic organization of the human insula has been investigated over the last century using postmortem brains but there has been little progress in noninvasive in vivo mapping of its microstruc...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53470
更新日期:2020-06-04 00:00:00
abstract::To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm genera...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03427
更新日期:2014-07-15 00:00:00
abstract::Meibum lipids form a lipid layer on the outermost side of the tear film and function to prevent water evaporation and reduce surface tension. (O-Acyl)-ω-hydroxy fatty acids (OAHFAs), a subclass of these lipids, are thought to be involved in connecting the lipid and aqueous layers in tears, although their actual functi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53582
更新日期:2020-04-07 00:00:00
abstract::Neural circuits that control copulation in male flies have been identified. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.22215
更新日期:2016-11-15 00:00:00
abstract::The yeast Dsc E3 ligase complex has long been recognized as a Golgi-specific protein ubquitination system. It shares a striking sequence similarity to the Hrd1 complex that plays critical roles in the ER-associated degradation pathway. Using biochemical purification and mass spectrometry, we identified two novel Dsc s...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.33116
更新日期:2018-01-22 00:00:00
abstract::Multiple lines of evidence suggest that functionally intact cerebello-hippocampal interactions are required for appropriate spatial processing. However, how the cerebellum anatomically and physiologically engages with the hippocampus to sustain such communication remains unknown. Using rabies virus as a retrograde tra...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.41896
更新日期:2019-06-17 00:00:00
abstract::The Cdc45/Mcm2-7/GINS (CMG) helicase separates DNA strands during replication in eukaryotes. How the CMG is assembled and engages DNA substrates remains unclear. Using electron microscopy, we have determined the structure of the CMG in the presence of ATPγS and a DNA duplex bearing a 3' single-stranded tail. The struc...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03273
更新日期:2014-08-12 00:00:00
abstract::An essential step for understanding the transcriptional circuits that control development and physiology is the global identification and characterization of regulatory elements. Here, we present the first map of regulatory elements across the development and ageing of an animal, identifying 42,245 elements accessible...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.37344
更新日期:2018-10-26 00:00:00
abstract::Mitochondria are dynamic organelles that must precisely control their protein composition according to cellular energy demand. Although nuclear-encoded mRNAs can be localized to the mitochondrial surface, the importance of this localization is unclear. As yeast switch to respiratory metabolism, there is an increase in...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57814
更新日期:2020-08-07 00:00:00
abstract::TMEM16A is a ligand-gated anion channel that is activated by intracellular Ca2+. This channel comprises two independent pores and closely apposed Ca2+ binding sites that are contained within each subunit of a homodimeric protein. Previously we characterized the influence of positively charged pore-lining residues on a...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.39122
更新日期:2018-10-12 00:00:00
abstract::When a human catches a ball, they estimate future target location based on the current trajectory. How animals, small and large, encode such predictive processes at the single neuron level is unknown. Here we describe small target-selective neurons in predatory dragonflies that exhibit localized enhanced sensitivity f...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26478
更新日期:2017-07-25 00:00:00
abstract::The voltage-gated K+ channel Kv2.1 serves a major structural role in the soma and proximal dendrites of mammalian brain neurons, tethering the plasma membrane (PM) to endoplasmic reticulum (ER). Although Kv2.1 clustering at neuronal ER-PM junctions (EPJs) is tightly regulated and highly conserved, its function remains...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49953
更新日期:2019-10-30 00:00:00