The distribution of fitness effects among synonymous mutations in a gene under directional selection.

abstract：:During development, neurons form synapses with their fate-determined targets. While we begin to elucidate the mechanisms by which extracellular ligand-receptor interactions enhance synapse specificity by inhibiting synaptogenesis, our knowledge about their intracellular mechanisms remains limited. Here we show that Ra...

journal_title：eLife

authors： Chen X,Shibata AC,Hendi A,Kurashina M,Fortes E,Weilinger NL,MacVicar BA,Murakoshi H,Mizumoto K

更新日期：2018-07-31 00:00:00

abstract：:Major depressive disorder (MDD) is a prevalent illness that can be precipitated by acute or chronic stress. Studies of patients with Wolfram syndrome and carriers have identified Wfs1 mutations as causative for MDD. The medial prefrontal cortex (mPFC) is known to be involved in depression and behavioral resilience, al...

journal_title：eLife

authors： Shrestha P,Mousa A,Heintz N

更新日期：2015-09-15 00:00:00

abstract：:A hallmark of CRISPR-Cas immunity systems is the CRISPR array, a genomic locus consisting of short, repeated sequences ('repeats') interspersed with short, variable sequences ('spacers'). CRISPR arrays are transcribed and processed into individual CRISPR RNAs that each include a single spacer, and direct Cas proteins ...

journal_title：eLife

authors： Stringer AM,Baniulyte G,Lasek-Nesselquist E,Seed KD,Wade JT

更新日期：2020-10-30 00:00:00

abstract：:A fluorescent imaging technique called fastFISH has been used to track the various steps involved in the transcription of a single DNA molecule. ...

journal_title：eLife

authors： Monnet J,Strick TR

更新日期：2014-01-01 00:00:00

abstract：:Dedifferentiation of acini to duct-like cells occurs during the physiologic damage response in the pancreas, but this process can be co-opted by oncogenic Kras to drive carcinogenesis. Myeloid cells infiltrate the pancreas during the onset of pancreatic cancer, and promote carcinogenesis. Here, we show that the functi...

journal_title：eLife

authors： Zhang Y,Yan W,Mathew E,Kane KT,Brannon A 3rd,Adoumie M,Vinta A,Crawford HC,Pasca di Magliano M

更新日期：2017-10-05 00:00:00

abstract：:Extracellular vesicles (EVs) encompass a variety of vesicles secreted into the extracellular space. EVs have been implicated in promoting tumor metastasis, but the molecular composition of tumor-derived EV sub-types and the mechanisms by which molecules are sorted into EVs remain mostly unknown. We report the separati...

journal_title：eLife

authors： Temoche-Diaz MM,Shurtleff MJ,Nottingham RM,Yao J,Fadadu RP,Lambowitz AM,Schekman R

更新日期：2019-08-22 00:00:00

abstract：:Cells can avoid the effects of so-called 'nonsense' mutations by several methods, including a newly discovered mechanism driven by microRNA molecules. ...

journal_title：eLife

authors： Jopling CL

更新日期：2014-09-09 00:00:00

abstract：:Post-transcriptional control by small regulatory RNA (sRNA) is critical for rapid adaptive processes. sRNAs can directly modulate mRNA degradation in Proteobacteria without interfering with translation. However, Firmicutes have a fundamentally different set of ribonucleases for mRNA degradation and whether sRNAs can r...

journal_title：eLife

authors： Durand S,Braun F,Helfer AC,Romby P,Condon C

更新日期：2017-04-24 00:00:00

abstract：:Linking interindividual differences in psychological phenotype to variations in brain structure is an old dream for psychology and a crucial question for cognitive neurosciences. Yet, replicability of the previously-reported 'structural brain behavior' (SBB)-associations has been questioned, recently. Here, we conduct...

journal_title：eLife

authors： Kharabian Masouleh S,Eickhoff SB,Hoffstaedter F,Genon S,Alzheimer's Disease Neuroimaging Initiative.

更新日期：2019-03-13 00:00:00

abstract：:The faithful segregation of eukaryotic chromosomes in mitosis requires that the genome be duplicated completely prior to anaphase. However, cells with large genomes sometimes fail to complete replication during interphase and instead enter mitosis with regions of incompletely replicated DNA. These regions are processe...

journal_title：eLife

authors： Sonneville R,Bhowmick R,Hoffmann S,Mailand N,Hickson ID,Labib K

更新日期：2019-09-23 00:00:00

abstract：:Two recently discovered transcription factors stop cells from dividing when plants face extreme heat and DNA damage. ...

journal_title：eLife

authors： Eekhout T,De Veylder L

更新日期：2019-04-09 00:00:00

abstract：:The >800 human G protein-coupled receptors (GPCRs) are responsible for transducing diverse chemical stimuli to alter cell state- and are the largest class of drug targets. Their myriad structural conformations and various modes of signaling make it challenging to understand their structure and function. Here, we devel...

journal_title：eLife

authors： Jones EM,Lubock NB,Venkatakrishnan AJ,Wang J,Tseng AM,Paggi JM,Latorraca NR,Cancilla D,Satyadi M,Davis JE,Babu MM,Dror RO,Kosuri S

更新日期：2020-10-21 00:00:00

abstract：:Adult neurogenesis in the hippocampus may represent a form of plasticity in brain functions including mood, learning and memory. However, mechanisms underlying neural stem/progenitor cells (NSPCs) proliferation are not well understood. We found that Agrin, a factor critical for neuromuscular junction formation, is ele...

journal_title：eLife

authors： Zhang H,Sathyamurthy A,Liu F,Li L,Zhang L,Dong Z,Cui W,Sun X,Zhao K,Wang H,Ho HH,Xiong WC,Mei L

更新日期：2019-07-03 00:00:00

abstract：: ...

journal_title：eLife

authors： DeAngelis BD,Zavatone-Veth JA,Clark DA

更新日期：2020-12-04 00:00:00

abstract：:Telomerase extends telomere sequences at chromosomal ends to protect genomic DNA. During this process it must select the correct nucleotide from a pool of nucleotides with various sugars and base pairing properties, which is critically important for the proper capping of telomeric sequences by shelterin. Unfortunately...

journal_title：eLife

authors： Schaich MA,Sanford SL,Welfer GA,Johnson SA,Khoang TH,Opresko PL,Freudenthal BD

更新日期：2020-06-05 00:00:00

abstract：:Journals are exploring new approaches to peer review in order to reduce bias, increase transparency and respond to author preferences. Funders are also getting involved. ...

journal_title：eLife

authors： Rodgers P

更新日期：2017-09-29 00:00:00

abstract：:IRE1α is an endoplasmic reticulum (ER) localized endonuclease activated by misfolded proteins in the ER. Previously, we demonstrated that IRE1α forms a complex with the Sec61 translocon, to which its substrate XBP1u mRNA is recruited for cleavage during ER stress (Plumb et al., 2015). Here, we probe IRE1α complexes in...

journal_title：eLife

authors： Sundaram A,Plumb R,Appathurai S,Mariappan M

更新日期：2017-05-15 00:00:00

abstract：:In addition to their role in desensitization and internalization of G protein-coupled receptors (GPCRs), β-arrestins are essential scaffolds linking GPCRs to Erk1/2 signaling. However, their role in GPCR-operated Erk1/2 activation differs between GPCRs and the underlying mechanism remains poorly characterized. Here, w...

journal_title：eLife

authors： Cassier E,Gallay N,Bourquard T,Claeysen S,Bockaert J,Crépieux P,Poupon A,Reiter E,Marin P,Vandermoere F

更新日期：2017-02-07 00:00:00

abstract：:The basolateral amygdala (BLA) is critical for associating initially neutral cues with appetitive and aversive stimuli and receives dense neuromodulatory acetylcholine (ACh) projections. We measured BLA ACh signaling and activity of neurons expressing CaMKIIα (a marker for glutamatergic principal cells) in mice during...

journal_title：eLife

authors： Crouse RB,Kim K,Batchelor HM,Girardi EM,Kamaletdinova R,Chan J,Rajebhosale P,Pittenger ST,Role LW,Talmage DA,Jing M,Li Y,Gao XB,Mineur YS,Picciotto MR

更新日期：2020-09-18 00:00:00

abstract：:The production of action sequences is a fundamental aspect of motor skills. To examine whether primary motor cortex (M1) is involved in maintenance of sequential movements, we trained two monkeys (Cebus apella) to perform two sequential reaching tasks. In one task, sequential movements were instructed by visual cues, ...

journal_title：eLife

authors： Ohbayashi M

更新日期：2020-02-10 00:00:00

abstract：:Determining the etiologic basis of the mutations that are responsible for cancer is one of the fundamental challenges in modern cancer research. Different mutational processes induce different types of DNA mutations, providing 'mutational signatures' that have led to key insights into cancer etiology. The most widely ...

journal_title：eLife

authors： Afsari B,Kuo A,Zhang Y,Li L,Lahouel K,Danilova L,Favorov A,Rosenquist TA,Grollman AP,Kinzler KW,Cope L,Vogelstein B,Tomasetti C

更新日期：2021-01-25 00:00:00

abstract：:The genome-scale transcriptional programs that specify the mammalian trachea and esophagus are unknown. Though NKX2-1 and SOX2 are hypothesized to be co-repressive master regulators of tracheoesophageal fates, this is untested at a whole transcriptomic scale and their downstream networks remain unidentified. By combin...

journal_title：eLife

authors： Kuwahara A,Lewis AE,Coombes C,Leung FS,Percharde M,Bush JO

更新日期：2020-06-09 00:00:00

abstract：:During cell locomotion and endocytosis, membrane-tethered WASP proteins stimulate actin filament nucleation by the Arp2/3 complex. This process generates highly branched arrays of filaments that grow toward the membrane to which they are tethered, a conflict that seemingly would restrict filament growth. Using three-c...

journal_title：eLife

authors： Smith BA,Padrick SB,Doolittle LK,Daugherty-Clarke K,Corrêa IR Jr,Xu MQ,Goode BL,Rosen MK,Gelles J

更新日期：2013-09-03 00:00:00

abstract：:Current models successfully describe the auditory cortical response to natural sounds with a set of spectro-temporal features. However, these models have hardly been linked to the ill-understood neurobiological changes that occur in the aging auditory cortex. Modelling the hemodynamic response to a rich natural sound ...

journal_title：eLife

authors： Erb J,Schmitt LM,Obleser J

更新日期：2020-07-03 00:00:00

abstract：:Cerebellar climbing fiber activity encodes performance errors during many motor learning tasks, but the role of these error signals in learning has been controversial. We compared two motor learning paradigms that elicited equally robust putative error signals in the same climbing fibers: learned increases and decreas...

journal_title：eLife

authors： Kimpo RR,Rinaldi JM,Kim CK,Payne HL,Raymond JL

更新日期：2014-04-22 00:00:00

abstract：:How different helicase families with a conserved catalytic 'helicase core' evolved to function on varied RNA and DNA substrates by diverse mechanisms remains unclear. In this study, we used Mss116, a yeast DEAD-box protein that utilizes ATP to locally unwind dsRNA, to investigate helicase specificity and mechanism. Ou...

journal_title：eLife

authors： Mallam AL,Sidote DJ,Lambowitz AM

更新日期：2014-12-12 00:00:00

abstract：:Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set of resources to streamline reporter gene knock-ins in zebrafish and demonstrate the broader utility of the method in mammalian cells. Our approach uses short homology of 24-...

journal_title：eLife

authors： Wierson WA,Welker JM,Almeida MP,Mann CM,Webster DA,Torrie ME,Weiss TJ,Kambakam S,Vollbrecht MK,Lan M,McKeighan KC,Levey J,Ming Z,Wehmeier A,Mikelson CS,Haltom JA,Kwan KM,Chien CB,Balciunas D,Ekker SC,Clark KJ,We

更新日期：2020-05-15 00:00:00

abstract：:Temporal patterning of neural progenitors leads to the sequential production of diverse neurons. To understand how extrinsic cues influence intrinsic temporal programs, we studied Drosophila mushroom body progenitors (neuroblasts) that sequentially produce only three neuronal types: γ, then α'β', followed by αβ. Oppos...

journal_title：eLife

authors： Rossi AM,Desplan C

更新日期：2020-07-06 00:00:00

abstract：:Intermittent hypoxia (IH) is the predominant pathophysiological disturbance in obstructive sleep apnea (OSA), known to be independently associated with cardiovascular diseases. However, the effect of IH on cardiac fibrosis and molecular events involved in this process are unclear. Here, we tested IH in angiotensin II ...

journal_title：eLife

authors： Bao Q,Zhang B,Suo Y,Liu C,Yang Q,Zhang K,Yuan M,Yuan M,Zhang Y,Li G

更新日期：2020-01-14 00:00:00

abstract：:Rod and cone photoreceptors are highly similar in many respects but they have important functional and molecular differences. Here, we investigate genome-wide patterns of DNA methylation and chromatin accessibility in mouse rods and cones and correlate differences in these features with gene expression, histone marks,...

journal_title：eLife

authors： Mo A,Luo C,Davis FP,Mukamel EA,Henry GL,Nery JR,Urich MA,Picard S,Lister R,Eddy SR,Beer MA,Ecker JR,Nathans J

更新日期：2016-03-07 00:00:00