Stop that nonsense!

abstract：:AMPARs control fast synaptic communication between neurons and their function relies on auxiliary subunits, which importantly modulate channel properties. Although it has been suggested that AMPARs can bind to TARPs with variable stoichiometry, little is known about the effect that this stoichiometry exerts on certain...

journal_title：eLife

authors： Miguez-Cabello F,Sánchez-Fernández N,Yefimenko N,Gasull X,Gratacòs-Batlle E,Soto D

更新日期：2020-05-26 00:00:00

abstract：:Mood and anxiety disorders are the most prevalent psychiatric conditions and are exacerbated by stress. Recent studies have suggested cyclooxygenase-2 (COX-2) inhibition could represent a novel treatment approach or augmentation strategy for affective disorders including anxiety disorders and major depression. We show...

journal_title：eLife

authors： Gamble-George JC,Baldi R,Halladay L,Kocharian A,Hartley N,Silva CG,Roberts H,Haymer A,Marnett LJ,Holmes A,Patel S

更新日期：2016-05-10 00:00:00

abstract：:In most animals, the brain controls the body via a set of descending neurons (DNs) that traverse the neck. DN activity activates, maintains or modulates locomotion and other behaviors. Individual DNs have been well-studied in species from insects to primates, but little is known about overall connectivity patterns acr...

journal_title：eLife

authors： Namiki S,Dickinson MH,Wong AM,Korff W,Card GM

更新日期：2018-06-26 00:00:00

abstract：:Early life adversity (ELA) is associated with increased risk for stress-related disorders later in life. The link between ELA and risk for psychopathology is well established but the developmental mechanisms remain unclear. Using a mouse model of resource insecurity, limited bedding (LB), we tested the effects of LB o...

journal_title：eLife

authors： Manzano Nieves G,Bravo M,Baskoylu S,Bath KG

更新日期：2020-07-21 00:00:00

abstract：:Actin dynamics generate forces to deform the membrane and overcome the cell's high turgor pressure during clathrin-mediated endocytosis (CME) in yeast, but precise molecular details are still unresolved. Our previous models predicted that actin filaments of the endocytic meshwork continually polymerize and disassemble...

journal_title：eLife

authors： Lacy MM,Baddeley D,Berro J

更新日期：2019-12-19 00:00:00

abstract：:The palmitoyl acyltransferase (PAT) ZDHHC14 is highly expressed in the hippocampus and is the only PAT predicted to bind Type-I PDZ domain-containing proteins. However, ZDHHC14's neuronal roles are unknown. Here, we identify the PDZ domain-containing Membrane-associated Guanylate Kinase (MaGUK) PSD93 as a direct ZDHHC...

journal_title：eLife

authors： Sanders SS,Hernandez LM,Soh H,Karnam S,Walikonis RS,Tzingounis AV,Thomas GM

更新日期：2020-11-13 00:00:00

abstract：:Eliminating malaria from a defined region involves draining the endemic parasite reservoir and minimizing local malaria transmission around imported malaria infections . In the last phases of malaria elimination, as universal interventions reap diminishing marginal returns, national resources must become increasingly ...

journal_title：eLife

authors： Reiner RC,Le Menach A,Kunene S,Ntshalintshali N,Hsiang MS,Perkins TA,Greenhouse B,Tatem AJ,Cohen JM,Smith DL

更新日期：2015-12-29 00:00:00

abstract：:Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remai...

journal_title：eLife

authors： Teves SS,An L,Bhargava-Shah A,Xie L,Darzacq X,Tjian R

更新日期：2018-06-25 00:00:00

abstract：:To initiate DNA replication, cells first load an MCM helicase double hexamer at origins in a reaction requiring ORC, Cdc6, and Cdt1, also called pre-replicative complex (pre-RC) assembly. The essential mechanistic role of Cdc6 ATP hydrolysis in this reaction is still incompletely understood. Here, we show that althoug...

journal_title：eLife

authors： Chang F,Riera A,Evrin C,Sun J,Li H,Speck C,Weinreich M

更新日期：2015-08-25 00:00:00

abstract：:Nuclear speckles (NS) are among the most prominent biomolecular condensates. Despite their prevalence, research on the function of NS is virtually restricted to colocalization analyses, since an organizing core, without which NS cannot form, remains unidentified. The monoclonal antibody SC35, raised against a spliceos...

journal_title：eLife

authors： Ilik İA,Malszycki M,Lübke AK,Schade C,Meierhofer D,Aktaş T

更新日期：2020-10-23 00:00:00

abstract：:The vertebral column or spine assembles around the notochord rod which contains a core made of large vacuolated cells. Each vacuolated cell possesses a single fluid-filled vacuole, and loss or fragmentation of these vacuoles in zebrafish leads to spine kinking. Here, we identified a mutation in the kinase gene dstyk tha...

journal_title：eLife

authors： Bagwell J,Norman J,Ellis K,Peskin B,Hwang J,Ge X,Nguyen SV,McMenamin SK,Stainier DY,Bagnat M

更新日期：2020-01-29 00:00:00

abstract：:Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In Caenorhabditis elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional equivalents across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role...

journal_title：eLife

authors： Rogala KB,Dynes NJ,Hatzopoulos GN,Yan J,Pong SK,Robinson CV,Deane CM,Gönczy P,Vakonakis I

更新日期：2015-05-29 00:00:00

abstract：:Malaria infection starts with injection of Plasmodium sporozoites by an Anopheles mosquito into the skin of the mammalian host. How sporozoites locate and enter a blood vessel is a critical, but poorly understood process. In this study, we examine sporozoite motility and their interaction with dermal blood vessels, us...

journal_title：eLife

authors： Hopp CS,Chiou K,Ragheb DR,Salman AM,Khan SM,Liu AJ,Sinnis P

更新日期：2015-08-13 00:00:00

abstract：:The structure of fitness landscapes is critical for understanding adaptive protein evolution. Previous empirical studies on fitness landscapes were confined to either the neighborhood around the wild type sequence, involving mostly single and double mutants, or a combinatorially complete subgraph involving only two am...

journal_title：eLife

authors： Wu NC,Dai L,Olson CA,Lloyd-Smith JO,Sun R

更新日期：2016-07-08 00:00:00

abstract：:Feedback inhibitory motifs are thought to be important for pattern separation across species. How feedback circuits may implement pattern separation of biologically plausible, temporally structured input in mammals is, however, poorly understood. We have quantitatively determined key properties of netfeedback inhibiti...

journal_title：eLife

authors： Braganza O,Mueller-Komorowska D,Kelly T,Beck H

更新日期：2020-02-20 00:00:00

abstract：:Hybrid sterility is one of the reproductive isolation mechanisms leading to speciation. Prdm9, the only known vertebrate hybrid-sterility gene, causes failure of meiotic chromosome synapsis and infertility in male hybrids that are the offspring of two mouse subspecies. Within species, Prdm9 determines the sites of pro...

journal_title：eLife

authors： Gregorova S,Gergelits V,Chvatalova I,Bhattacharyya T,Valiskova B,Fotopulosova V,Jansa P,Wiatrowska D,Forejt J

更新日期：2018-03-14 00:00:00

abstract：:The piriform cortex (PCx) receives direct input from the olfactory bulb (OB) and is the brain's main station for odor recognition and memory. The transformation of the odor code from OB to PCx is profound: mitral and tufted cells in olfactory glomeruli respond to individual odorant molecules, whereas pyramidal neurons...

journal_title：eLife

authors： Kumar A,Schiff O,Barkai E,Mel BW,Poleg-Polsky A,Schiller J

更新日期：2018-12-21 00:00:00

abstract：:In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well understood. To understand the time-dependent scaling dynamics we treated cultured rat hippocampal cells wi...

journal_title：eLife

authors： Schanzenbächer CT,Langer JD,Schuman EM

更新日期：2018-02-15 00:00:00

abstract：:D2 autoreceptors regulate dopamine release throughout the brain. Two isoforms of the D2 receptor, D2S and D2L, are expressed in midbrain dopamine neurons. Differential roles of these isoforms as autoreceptors are poorly understood. By virally expressing the isoforms in dopamine neurons of D2 receptor knockout mice, th...

journal_title：eLife

authors： Gantz SC,Robinson BG,Buck DC,Bunzow JR,Neve RL,Williams JT,Neve KA

更新日期：2015-08-26 00:00:00

abstract：:Two-dimensional (2D) human skeletal muscle fiber cultures are ill-equipped to support the contractile properties of maturing muscle fibers. This limits their application to the study of adult human neuromuscular junction (NMJ) development, a process requiring maturation of muscle fibers in the presence of motor neuron...

journal_title：eLife

authors： Afshar Bakooshli M,Lippmann ES,Mulcahy B,Iyer N,Nguyen CT,Tung K,Stewart BA,van den Dorpel H,Fuehrmann T,Shoichet M,Bigot A,Pegoraro E,Ahn H,Ginsberg H,Zhen M,Ashton RS,Gilbert PM

更新日期：2019-05-14 00:00:00

abstract：:Group B Streptococcus (GBS) is the leading cause of invasive bacterial neonatal infections. Late-onset diseases (LOD) occur between 7 and 89 days of life and are largely due to the CC17 GBS hypervirulent clone. We studied the impact of estradiol (E2) and progesterone (P4), which impregnate the fetus during pregnancy, ...

journal_title：eLife

authors： Hays C,Touak G,Bouaboud A,Fouet A,Guignot J,Poyart C,Tazi A

更新日期：2019-11-11 00:00:00

abstract：:Direct activation of the human phospholipase C-γ isozymes (PLC-γ1, -γ2) by tyrosine phosphorylation is fundamental to the control of diverse biological processes, including chemotaxis, platelet aggregation, and adaptive immunity. In turn, aberrant activation of PLC-γ1 and PLC-γ2 is implicated in inflammation, autoimmu...

journal_title：eLife

authors： Hajicek N,Keith NC,Siraliev-Perez E,Temple BR,Huang W,Zhang Q,Harden TK,Sondek J

更新日期：2019-12-31 00:00:00

abstract：:Although pain is a common symptom of various diseases and disorders, its contribution to disease pathogenesis is not well understood. Here we show using murine experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis (MS), that pain induces EAE relapse. Mechanistic analysis showed that pain indu...

journal_title：eLife

authors： Arima Y,Kamimura D,Atsumi T,Harada M,Kawamoto T,Nishikawa N,Stofkova A,Ohki T,Higuchi K,Morimoto Y,Wieghofer P,Okada Y,Mori Y,Sakoda S,Saika S,Yoshioka Y,Komuro I,Yamashita T,Hirano T,Prinz M,Murakami M

更新日期：2015-08-11 00:00:00

abstract：:Nucleolar protein interacting with the FHA domain of pKi-67 (NIFK) is a Ki-67-interacting protein. However, its precise function in cancer remains largely uninvestigated. Here we show the clinical significance and metastatic mechanism of NIFK in lung cancer. NIFK expression is clinically associated with poor prognosis...

journal_title：eLife

authors： Lin TC,Su CY,Wu PY,Lai TC,Pan WA,Jan YH,Chang YC,Yeh CT,Chen CL,Ger LP,Chang HT,Yang CJ,Huang MS,Liu YP,Lin YF,Shyy JY,Tsai MD,Hsiao M

更新日期：2016-03-17 00:00:00

abstract：:RNA-protein (RNP) granules have been proposed to assemble by forming solid RNA/protein aggregates or through phase separation into a liquid RNA/protein phase. Which model describes RNP granules in living cells is still unclear. In this study, we analyze P bodies in budding yeast and find that they have liquid-like pro...

journal_title：eLife

authors： Kroschwald S,Maharana S,Mateju D,Malinovska L,Nüske E,Poser I,Richter D,Alberti S

更新日期：2015-08-04 00:00:00

abstract：:While host immune receptors detect pathogen-associated molecular patterns to activate immunity, pathogens attempt to deregulate host immunity through secreted effectors. Fungi employ LysM effectors to prevent recognition of cell wall-derived chitin by host immune receptors, although the mechanism to compete for chitin...

journal_title：eLife

authors： Sánchez-Vallet A,Saleem-Batcha R,Kombrink A,Hansen G,Valkenburg DJ,Thomma BP,Mesters JR

更新日期：2013-07-02 00:00:00

abstract：:Neuropathic pain remains notoriously difficult to treat despite numerous drug targets. Here, we offer a novel explanation for this intractability. Computer simulations predicted that qualitative changes in primary afferent excitability linked to neuropathic pain arise through a switch in spike initiation dynamics when...

journal_title：eLife

authors： Ratté S,Zhu Y,Lee KY,Prescott SA

更新日期：2014-04-01 00:00:00

abstract：:During outbreaks of high-consequence pathogens, airport screening programs have been deployed to curtail geographic spread of infection. The effectiveness of screening depends on several factors, including pathogen natural history and epidemiology, human behavior, and characteristics of the source epidemic. We develop...

journal_title：eLife

authors： Gostic KM,Kucharski AJ,Lloyd-Smith JO

更新日期：2015-02-19 00:00:00

abstract：:Activation of systemic acquired resistance in plants is associated with transcriptome reprogramming induced by the unstable coactivator NPR1. Immune-induced ubiquitination and proteasomal degradation of NPR1 are thought to facilitate continuous delivery of active NPR1 to target promoters, thereby maximising gene expre...

journal_title：eLife

authors： Skelly MJ,Furniss JJ,Grey H,Wong KW,Spoel SH

更新日期：2019-10-07 00:00:00

abstract：:This study examined records of 2566 consecutive COVID-19 patients at five Massachusetts hospitals and sought to predict level-of-care requirements based on clinical and laboratory data. Several classification methods were applied and compared against standard pneumonia severity scores. The need for hospitalization, IC...

journal_title：eLife

authors： Hao B,Sotudian S,Wang T,Xu T,Hu Y,Gaitanidis A,Breen K,Velmahos GC,Paschalidis IC

更新日期：2020-10-12 00:00:00