Abstract:
:Activation of systemic acquired resistance in plants is associated with transcriptome reprogramming induced by the unstable coactivator NPR1. Immune-induced ubiquitination and proteasomal degradation of NPR1 are thought to facilitate continuous delivery of active NPR1 to target promoters, thereby maximising gene expression. Because of this potentially costly sacrificial process, we investigated if ubiquitination of NPR1 plays transcriptional roles prior to its proteasomal turnover. Here we show ubiquitination of NPR1 is a progressive event in which initial modification by a Cullin-RING E3 ligase promotes its chromatin association and expression of target genes. Only when polyubiquitination of NPR1 is enhanced by the E4 ligase, UBE4, it is targeted for proteasomal degradation. Conversely, ubiquitin ligase activities are opposed by UBP6/7, two proteasome-associated deubiquitinases that enhance NPR1 longevity. Thus, immune-induced transcriptome reprogramming requires sequential actions of E3 and E4 ligases balanced by opposing deubiquitinases that fine-tune activity of NPR1 without strict requirement for its sacrificial turnover.
journal_name
Elifejournal_title
eLifeauthors
Skelly MJ,Furniss JJ,Grey H,Wong KW,Spoel SHdoi
10.7554/eLife.47005subject
Has Abstractpub_date
2019-10-07 00:00:00issn
2050-084Xpii
47005journal_volume
8pub_type
杂志文章相关文献
eLife文献大全abstract::Aging is characterized by extensive metabolic reprogramming. To identify metabolic pathways associated with aging, we analyzed age-dependent changes in the metabolomes of long-lived Drosophila melanogaster. Among the metabolites that changed, levels of tyrosine were increased with age in long-lived flies. We demonstra...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58053
更新日期:2020-12-15 00:00:00
abstract::Seasonal influenza virus A/H3N2 is a major cause of death globally. Vaccination remains the most effective preventative. Rapid mutation of hemagglutinin allows viruses to escape adaptive immunity. This antigenic drift necessitates regular vaccine updates. Effective vaccine strains need to represent H3N2 populations ci...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60067
更新日期:2020-09-02 00:00:00
abstract::The ubiquitous presence of inhibitory interneurons in the thalamus of primates contrasts with the sparsity of interneurons reported in mice. Here, we identify a larger than expected complexity and distribution of interneurons across the mouse thalamus, where all thalamic interneurons can be traced back to two developm...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59272
更新日期:2021-02-01 00:00:00
abstract::Optical imaging has become a powerful tool for studying brains in vivo. The opacity of adult brains makes microendoscopy, with an optical probe such as a gradient index (GRIN) lens embedded into brain tissue to provide optical relay, the method of choice for imaging neurons and neural activity in deeply buried brain s...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40805
更新日期:2019-01-04 00:00:00
abstract::How living systems break symmetry in an organized manner is a fundamental question in biology. In wild-type Caenorhabditis elegans zygotes, symmetry breaking during anterior-posterior axis specification is guided by centrosomes, resulting in anterior-directed cortical flows and a single posterior PAR-2 domain. We unco...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44552
更新日期:2019-02-26 00:00:00
abstract::Photosynthetic starch reserves that accumulate in Arabidopsis leaves during the day decrease approximately linearly with time at night to support metabolism and growth. We find that the rate of decrease is adjusted to accommodate variation in the time of onset of darkness and starch content, such that reserves last al...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00669
更新日期:2013-06-25 00:00:00
abstract::Cognitive functions such as working memory (WM) are emergent properties of large-scale network interactions. Synchronisation of oscillatory activity might contribute to WM by enabling the coordination of long-range processes. However, causal evidence for the way oscillatory activity shapes network dynamics and behavio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22001
更新日期:2017-03-14 00:00:00
abstract::Events in early life contribute to subsequent risk of asthma; however, the causes and trajectories of childhood wheeze are heterogeneous and do not always result in asthma. Similarly, not all atopic individuals develop wheeze, and vice versa. The reasons for these differences are unclear. Using unsupervised model-base...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.35856
更新日期:2018-10-15 00:00:00
abstract::How does the skeleton detect and adapt to changes in the mechanical load it has to carry? ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.50210
更新日期:2019-10-07 00:00:00
abstract::During inflammation, serum amyloid A proteins transport retinol to infected tissues. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.04246
更新日期:2014-09-02 00:00:00
abstract::The speed at which a cell fate decision in nematode worms evolves is due to the number of genes that control the decision, rather than to a high mutation rate. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.62689
更新日期:2020-10-14 00:00:00
abstract::Variation in the presentation of hereditary immunodeficiencies may be explained by genetic or environmental factors. Patients with mutations in HOIL1 (RBCK1) present with amylopectinosis-associated myopathy with or without hyper-inflammation and immunodeficiency. We report that barrier-raised HOIL-1-deficient mice exh...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04494
更新日期:2015-01-20 00:00:00
abstract::The NuRD complex subunit CHD4 is essential for fusion-positive rhabdomyosarcoma (FP-RMS) survival, but the mechanisms underlying this dependency are not understood. Here, a NuRD-specific CRISPR screen demonstrates that FP-RMS is particularly sensitive to CHD4 amongst the NuRD members. Mechanistically, NuRD complex con...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54993
更新日期:2020-08-03 00:00:00
abstract::Toxoplasma gondii contains a limited subset of actin binding proteins. Here we show that the putative actin regulator cyclase-associated protein (CAP) is present in two different isoforms and its deletion leads to significant defects in some but not all actin dependent processes. We observe defects in cell-cell commun...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50598
更新日期:2019-10-02 00:00:00
abstract::Actin has well established functions in cellular morphogenesis. However, it is not well understood how the various actin assemblies in a cell are kept in a dynamic equilibrium, in particular when cells have to respond to acute signals. Here, we characterize a rapid and transient actin reset in response to increased in...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19850
更新日期:2016-12-06 00:00:00
abstract::Hippo signalling integrates diverse stimuli related to epithelial architecture to regulate tissue growth and cell fate decisions. The Hippo kinase cascade represses the growth-promoting transcription co-activator Yorkie. The FERM protein Expanded is one of the main upstream Hippo signalling regulators in Drosophila as...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46592
更新日期:2019-09-30 00:00:00
abstract::A small transparent crustacean called Parhyale hawaiensis has become a powerful model system for the study of limb and appendage regeneration. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.21583
更新日期:2016-10-25 00:00:00
abstract::Repression of genes by Polycomb requires that PRC2 modifies their chromatin by trimethylating lysine 27 on histone H3 (H3K27me3). At transcriptionally active genes, di- and tri-methylated H3K36 inhibit PRC2. Here, the cryo-EM structure of PRC2 on dinucleosomes reveals how binding of its catalytic subunit EZH2 to nucle...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.61964
更新日期:2020-11-19 00:00:00
abstract::Macrophages derive from multiple sources of hematopoietic progenitors. Most macrophages require colony-stimulating factor 1 receptor (CSF1R), but some macrophages persist in the absence of CSF1R. Here, we analyzed mpeg1:GFP-expressing macrophages in csf1r-deficient zebrafish and report that embryonic macrophages emerg...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53403
更新日期:2020-05-05 00:00:00
abstract::Lipoprotein lipase (LPL) undergoes spontaneous inactivation via global unfolding and this unfolding is prevented by GPIHBP1 (Mysling et al., 2016). We now show: (1) that ANGPTL4 inactivates LPL by catalyzing the unfolding of its hydrolase domain; (2) that binding to GPIHBP1 renders LPL largely refractory to this inhib...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20958
更新日期:2016-12-08 00:00:00
abstract::B-cell receptors form ordered clusters to recruit kinases and exclude phosphatases. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.25375
更新日期:2017-03-30 00:00:00
abstract::Two gene variants provide different levels of protection against sleeping sickness, but this comes with an increased risk of developing chronic kidney disease. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.29056
更新日期:2017-07-03 00:00:00
abstract::Adhesion molecules hold cells together but also couple cell membranes to a contractile actomyosin network, which limits the expansion of cell contacts. Despite their fundamental role in tissue morphogenesis and tissue homeostasis, how adhesion molecules control cell shapes and cell patterns in tissues remains unclear....
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22796
更新日期:2017-05-24 00:00:00
abstract::Newly born cells either continue to proliferate or exit the cell division cycle. This decision involves delaying expression of Cyclin E that promotes DNA replication. ORC1, the Origin Recognition Complex (ORC) large subunit, is inherited into newly born cells after it binds to condensing chromosomes during the precedi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12785
更新日期:2016-07-26 00:00:00
abstract::Disruption of the sumoylation/desumoylation equilibrium is associated with several disease states such as cancer and infections, however the mechanisms regulating the global SUMO balance remain poorly defined. Here, we show that infection by Shigella flexneri, the causative agent of human bacillary dysentery, switches...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27444
更新日期:2017-12-12 00:00:00
abstract::The COVID-19 pandemic has resulted in school closures and distancing requirements that have disrupted both work and family life for many. Concerns exist that these disruptions caused by the pandemic may not have influenced men and women researchers equally. Many medical journals have published papers on the pandemic, ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58807
更新日期:2020-06-15 00:00:00
abstract::The TAM receptor tyrosine kinases Tyro3, Axl, and Mer regulate key features of cellular physiology, yet the differential activities of the TAM ligands Gas6 and Protein S are poorly understood. We have used biochemical and genetic analyses to delineate the rules for TAM receptor-ligand engagement and find that the TAMs...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03385
更新日期:2014-09-29 00:00:00
abstract::The piriform cortex (PCx) receives direct input from the olfactory bulb (OB) and is the brain's main station for odor recognition and memory. The transformation of the odor code from OB to PCx is profound: mitral and tufted cells in olfactory glomeruli respond to individual odorant molecules, whereas pyramidal neurons...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.38446
更新日期:2018-12-21 00:00:00
abstract:Background:Malaria elimination strategies require a thorough understanding of parasite transmission from human to mosquito. A clinical model to induce gametocytes to understand their dynamics and evaluate transmission-blocking interventions (TBI) is currently unavailable. Here, we explore the use of the well-establishe...
journal_title:eLife
pub_type: 杂志文章,随机对照试验
doi:10.7554/eLife.31549
更新日期:2018-02-27 00:00:00
abstract::Recurrent somatic mutations of H3F3A in aggressive pediatric high-grade gliomas generate K27M or G34R/V mutant histone H3.3. H3.3-G34R/V mutants are common in tumors with mutations in p53 and ATRX, an H3.3-specific chromatin remodeler. To gain insight into the role of H3-G34R, we generated fission yeast that express o...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27406
更新日期:2017-07-18 00:00:00